EMAGE mouse gene expression spatial database

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EMAGE:10591

Smtn smoothelin ( MGI:1354727)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000906. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000906_01	euxassay_000906_02	euxassay_000906_03	euxassay_000906_04

euxassay_000906_05	euxassay_000906_06	euxassay_000906_07	euxassay_000906_08	euxassay_000906_09

euxassay_000906_10	euxassay_000906_11	euxassay_000906_12	euxassay_000906_13	euxassay_000906_14

euxassay_000906_15	euxassay_000906_16	euxassay_000906_17	euxassay_000906_18	euxassay_000906_19

euxassay_000906_20	euxassay_000906_21	euxassay_000906_22	euxassay_000906_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10591_wholemount_strong.wlz

10591_wholemount_moderate.wlz

10591_wholemount_weak.wlz

10591_wholemount_possible.wlz

10591_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10591_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 20 21 22 23

vertebral axis musculature

moderate

homogeneous

moderate expression: see section 01 02 03 11 12 13 14 15 16 17 18 19 20 21

alimentary system

strong

spotted

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2175
Entity Detected:	Smtn, smoothelin ( MGI:1354727)
Sequence:	sense strand is shown >T2175 TGGCCTCGAGCAGATTCGGATCCACCCANCCCTTCTCAGCACCAGCAGTGGGGGCAAGAACACCATCACC CACATCAGCAACCCTGGGACTGTGACCCGACTGGGCAGTGTCACTCACGTCACTACCTTCAGCCATGCCT CCCCTGGTAACCGAGGAGGCTGCAACTTTAAGATGGAGCCAGATCCTGCAGAGCCCCCCTCCACCACAGT GGAAGCAGCTAATGGCGCAGAGCAGGCTCGAGTGGACAAAGGCCCAGAGGGGCGGAGTCCCCTGAGTGCA GAGGAGCTGACGGCCATTGAGGACGAAGGAGTCCTGGACAAGATGCTGGACCAGACTACGAACTTTGAGG AGAGGAAGCTCATCCGGGCTGCACTGCGTGAGCTCCGACAAAGAAAGAGAGACCAGAGGGACAAGGAACG AGAACGGAGGCTACGGGAGGCACGGGCCCGGCCGGGCGAGAGCCGAAGCAATGTGGCCACGGAGACCACC ACCAGGCACAGCCAGCGGGCGGCTGATGGCTCTACTGTCGGCACAGTT
Notes:	The probe template was PCR amplified from IMAGE:889310 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:889310 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10592 same embryo
	EMAGE:10588 same embryo
	EMAGE:10590 same embryo
	EMAGE:10587 same embryo
	EMAGE:10589 same embryo
	EurExpress:euxassay_000906 same experiment
	MGI:4828337 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source