EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10638

Cabp4 calcium binding protein 4 ( MGI:1920910)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003245. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003245_01	euxassay_003245_02	euxassay_003245_03	euxassay_003245_04

euxassay_003245_05	euxassay_003245_06	euxassay_003245_07	euxassay_003245_08	euxassay_003245_09

euxassay_003245_10	euxassay_003245_11	euxassay_003245_12	euxassay_003245_13	euxassay_003245_14

euxassay_003245_15	euxassay_003245_16	euxassay_003245_17	euxassay_003245_18	euxassay_003245_19

euxassay_003245_20	euxassay_003245_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10638_wholemount_strong.wlz

10638_wholemount_moderate.wlz

10638_wholemount_weak.wlz

10638_wholemount_possible.wlz

10638_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10638_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19 20 weak expression: see section 08

not examined

regional

not examined expression: see section 01 02 20 21

neural retina

weak

regional

weak expression: see section 01 02 20 21

lung

moderate

regional

moderate expression: see section 05 07 08 09 10 13 14 15 16 17 18 19 20 weak expression: see section 02 03 04 06

tail dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 12 14 15 16 weak expression: see section 11 13 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5409
Entity Detected:	Cabp4, calcium binding protein 4 ( MGI:1920910)
Sequence:	sense strand is shown >T5409 GAGAAGCAAGGGCACTCCAGGGAGCTACATCACTGAGTCCTGTTATCTTCTCCATGGCAACAGAGCACAA TGTACAGCTGGTTCCTGGCTCCCAGAAGATCCCTAAAGGAGTTGTATCTCCCAGGAGTGCTGCTGAGGGC CCTGCCTTGACCAGGAGAAGGAGCAAGAAGGAGAGTTGGCACCCAGGGTCCCAGAAAGCCAGTTCTGGGG ACCAGTCCTCTAGCCAGGGCTCTGAGGCCTCAGGAAGCAGCAAGCACCCCCCAAGGACCAAAGTGGGGCA GGAGGAACCATCCTCAGCCCCCGCCAGGCCAGCGTCTCACCGACACTCCCATCGACACCGTTCTGACCCC CAGCAGGATGCTGCCCAAAGGACTTATGGGCCCTTGCTCAACCGCATGTTTGGAAAGGATCGTGAACTGG GCCCTGAGGAGTTGGAGGAGCTCCAGGCTGCCTTTGAGGAGTTTGACACTGACCAGGACGGCTACATCGG CTACCGCGAGCTAGGTGACTGCATGCGGACGCTGGGCTACATGCCCACGGAGATGGAGCTTCTAGAAGTG TCGCAGCACGTGAAGATGCGCATGGGAGGCTTTGTGGATTTTGAAGAGTTTGTGGAGCTGATAAGCCCAA AGCTGAGGGAGGAGACAGCTCACATGCTGGGCGTTCGGGAGCTACGCATCGCCTTCCGAGAGTTTGACAA GGACAGGGATGGACGGATCACGGTGGCGGAGCTGCGGCAGGCAGCACCAGCTCTGCTGGGGGAACCACTA GAGGGCACTGAACTGGATGAGATGTTGCGAGAAATGGACCTCAATGGGGATGGCACCATAGACTTTGACG AGTTTGTAATGATGCTATCTACAGGCTGAGGCACCTTCAGGGACAACCTACTGGCCCTGGAGGCCAAGAC ACCAGCAGACACCAGACTACCACACCCTTCCCCAGCATGGAGAAATCCCCCTTCCCCCAGGCTGGTTCAC CCCCTCACCACACCCAACTCCAGAGTCTGCCTGTGCTGTCTTCTCCCATCCCCAAGCTGTGGAGAGAAAA AAAAAATTGAAGCTGGTTACAGTTTTCACTGGAGAACATGATTTGAATCTCTTTAGATCCTGGAGAGGTA GGGAGTTCCCCACAACTGGCTGCATTCAAATGAATGAGGCATAGTGCATTGGGGGAATTCCTAGGACATG ATGAATTGAGAGAGTAGCTAGATCACTTCCCACTACTTGTTTGTATAAAGGGTTGTTTTTGAAAAATGAA CTTGGGCCTGGTAATGACTTTGTAGGTAAAAGTTTATGCCACTAAGCCTGAATTTGATCCCTGAAGTTTG TGTGGTGGAATGAGAGAACCAACTTCACAAAGTTGCTCTTTGGTAGGCTAATGCCCTGTTCACACCCCCT TGAATAAATAAATAAATGAAAAGTGAACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:5366999 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5366999 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10641 same embryo
	EMAGE:10637 same embryo
	EMAGE:10642 same embryo
	EMAGE:10640 same embryo
	EMAGE:10639 same embryo
	EurExpress:euxassay_003245 same experiment
	MGI:4823570 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source