Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10684

Kctd11 potassium channel tetramerisation domain containing 11 ( MGI:2448712)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10684 EMAGE:10684 EMAGE:10684 EMAGE:10684 EMAGE:10684
"Pseudo-wholemount" of euxassay_000883. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_000883_01 euxassay_000883_02 euxassay_000883_03 euxassay_000883_04
EMAGE:10684 EMAGE:10684 EMAGE:10684 EMAGE:10684 EMAGE:10684
euxassay_000883_05 euxassay_000883_06 euxassay_000883_07 euxassay_000883_08 euxassay_000883_09
EMAGE:10684 EMAGE:10684 EMAGE:10684 EMAGE:10684 EMAGE:10684
euxassay_000883_10 euxassay_000883_11 euxassay_000883_12 euxassay_000883_13 euxassay_000883_14
EMAGE:10684 EMAGE:10684 EMAGE:10684 EMAGE:10684 EMAGE:10684
euxassay_000883_15 euxassay_000883_16 euxassay_000883_17 euxassay_000883_18 euxassay_000883_19
EMAGE:10684 EMAGE:10684 EMAGE:10684 EMAGE:10684
euxassay_000883_20 euxassay_000883_21 euxassay_000883_22 euxassay_000883_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10684Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10684_wholemount_strong.wlz
10684_wholemount_moderate.wlz
10684_wholemount_weak.wlz
10684_wholemount_possible.wlz
10684_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10684_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
liver lobe
moderate moderate
single cellmoderate expression: see section 01 02 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T201
Entity Detected:Kctd11, potassium channel tetramerisation domain containing 11 ( MGI:2448712)
Sequence:sense strand is shown

>T201
GAAGGCGGTAACGCCCGAGCCCTGAGACACTGTTCCCAACAGAGTCCCGGGACACTCTCAGCTCTGACTG
CAAGAGCATCGCCTCTCCCAAGCCATACAGAACTGCTGGCTACCGGCTCCTATCAGTTGCGCCTCCATCT
TTTGGGACACACGCCCCTAACCCTGGCTGGGCCTCTGAACAGGTCCTCGGAACCCGACGTGGACTCTACA
ACTACAAAAGCTCAGAAACTCAAACTGATCCTTTCAGCGTCCGACCGGTGCTCACCACCGTCACCTGCTG
GTTGAATTTCGGAAACCCACTGTCTGAAGAGTACAGGAGGGAATCGCTGACCACGCACAATCAGAAGCGT
CTGGACCAAAAGCTCCCTGATCCTGTCTGGCTGCCGTCTGTTCCTTTCATCGGTTCTTTCATCCTCTCGG
GGATTTTTGTGTCCTGAAGCCCCCAACCACCACCAAAGTAGCTTGGGTGAGCTACAAACTTTACT
Notes:The probe template was PCR amplified from IMAGE:2648499 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2648499 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10687 same embryo
 EMAGE:10686 same embryo
 EMAGE:10685 same embryo
 EMAGE:10683 same embryo
 EMAGE:10682 same embryo
 EurExpress:euxassay_000883 same experiment
 MGI:4825735 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS