EMAGE mouse gene expression spatial database

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EMAGE:10710

Glp2r glucagon-like peptide 2 receptor ( MGI:2136733)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003193. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003193_01	euxassay_003193_02	euxassay_003193_03	euxassay_003193_04

euxassay_003193_05	euxassay_003193_06	euxassay_003193_07	euxassay_003193_08	euxassay_003193_09

euxassay_003193_10	euxassay_003193_11	euxassay_003193_12	euxassay_003193_13	euxassay_003193_14

euxassay_003193_15	euxassay_003193_16	euxassay_003193_17	euxassay_003193_18	euxassay_003193_19

euxassay_003193_20	euxassay_003193_21	euxassay_003193_22	euxassay_003193_23	euxassay_003193_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10710_wholemount_strong.wlz

10710_wholemount_moderate.wlz

10710_wholemount_weak.wlz

10710_wholemount_possible.wlz

10710_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10710_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

moderate

regional

moderate expression: see section 13 14 15 16 17 18

hindgut

moderate

regional

moderate expression: see section 13 14 15 16 17 18

rectum

moderate

regional

moderate expression: see section 15

midgut

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 weak expression: see section 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5363
Entity Detected:	Glp2r, glucagon-like peptide 2 receptor ( MGI:2136733)
Sequence:	sense strand is shown >T5363 GATTCGGTAGATCGCTGTAGAGCAACTCAGACAGTCGGCGGCCTGAAGAGGACTTGTGCAAACACTTCCT CTCTGGACAAGGAGGAATGCAGGAGGCCACCGCCTGCAGTACATCTTGGAGTGTTGGAGGGATGTGCCTG CACTTGTGAAAGGGCGCCAGAAGGACGAGGCCCCAACCAAGCCCGGCAGTGCCCAGTAGATGCAGAGAGG CGTCCCTGCCCCGGGCGCAGCCAGTGGGCATCCCTGCGGCCCAGGGGCCTGAGTCTCTCCACTCCCACGG GATGCGTCGGCTCTGGGGCCCTGGGACGCCCTTCCTCGCCCTGCTTCTGCTGGTTTCCATCAAGCAAGTT ACTACAGGATCTCTCCTTGAGGAGACAGTTCAGAAGTGGGCTCAGTATAAGGAGACGTGTCTGAAAGACT TGCTCGAGAAACCTTCTGGTGTATTTTGTAATGGAACATTTGATAAGTACGTGTGCTGGCCTCATTCTTT TCCTGGAAATGTCTCTGTCCCCTGTCCTTCATACTTACCTTGGTGGAATAAAGAGAGCCCAGGAAGGGCC TACAGACACTGCTTGGCTCAGGGGACTTGGCAGAAGCAAGAGAACTCCACAGATACTTGGCAGGATGAGT CAGAATGCTCAGAGAACCACAGCTTCAAACAAAACGTGGATCACTATCACCATACCTTGTTGTCCACCTT GCAGCTGATGTACACTGTGGGCTACTCCCTGTCTCTCATCTCCCTCTTCTTGGCTCTTACACTCTTCTTG TTCCTTCGAAAACTGCACTGCACACGCAATTACATCCACATGAACCTGTTCGCTTCGTTCATCCTGAGAG CTCTGGTTGTCCTTGTGAAGGACATGGTCTTCTACAACTCTTACTCCAGGAGGCCTGACAGTGAGAGTGG ATGGATGTCATATCTGTCAGAGATTTCTGCCTCCTGCCGCTCTGTCCAGGTCCTCCTGCACTACTTTGTG GGCACCAATCACTTGTGGTTGCTGGTTGAAGGACTTTACCTCCATGCTCTGCTGGAGCCAACAGTGCTTC CTGAAAGGCGGCTGTGGCCCAAGTACCTGGTGGTGGGTTGGGCCTTCCCCATGCTGTTTGTTATTCCCTG GATTTTTGTCCGAGCAAGCCTGGAGAATACAGGGTGCTGGGCAGTAAATGAGAATAAGAAAATCTGGTGG ATCATCAGAGGACCCATTCTGCTTTGTGTAACAGTTAATTTCTTCATCTTCCTCAAGATTCTCAAGCTTC TCATTTCTAAGTTCAGAGCTCATCAGATGTGCTTCAGAGACTACAAATATAGACTGGCAAAATCAACCCT GCTCCTCATTCTTTTGATGGGGGTTCATGAGTTCCTCTTCACTTTCTTCACCGACGACCAAGTTCAAGGA TTTTCAAGACTTATTCGTCTCTTCATCCAGTTGACCCTGAGCTCCTTCCATGGATTTCTGGTGGCCTTGC AGTACGGCTTTGCCAGTAGAGAGGTGAAGGCAGAGCTGAGAAAGACCTGGGGCCGCTTCTTATTAGCTCG CCACTGGGGCTGCAGAGCCTGTGTCCTAGGGAAGAACTTCCGGTTCCTGGGGAAATGTTCCAAGAAGCTG TCGGAGGGAGATGGTGCCGAGACACTCCAGAAGCTGCAATCTTCAGGTGTCAGCTCACACCTGACCGCTG GGAACCTGCGGGATCATGGGGCACAGCCTCACAGGGGCCGTGGAGCTTGGCCCCGGGCAAGCAGCCTGTC TGAGAGCAGCGAGGGAGACTTCACCCTGGCCAATACCATGGAGGAGATTCTGGAAGAGAGTGAGATCTAA GACAGGGTCCATCACCACAGCTTGGCAACTGAGGATCCCTAGGAAAGAGGTTGCTGAGCCCAGGGTCCTC CTCTTTCCATTCACTATCCCCATTTTGATGTGAAGTCTCTTCTGGGTTATCAAGCTCCATATCAGTGAGT CTATATAATGCCCACAGTCCCTCCTGCCTTTGTCACCCTACCCCCCCCCACCCCCACCATGCATATTTTC CAGAATGTCCGTTGGTTTTGGGGGCCTTGCCCTAAATTCAAGTGAGTGGAACCCACCATGAAGAAAAATC ATTTATTAAATAGGGTTCAGTTTGTATCTCCTTCCCGTTCATGGTGTATGGCTTCCTTCTAGAGATGGAA GTAGGGTGCACTGGAACCCCACAGGAAGCTTTAAAGTATCAAGTTCTGGGGAATCACAGCAAATATTCTA AGAGAGAAAGTCTGAGATGAGGACCAAGAATTGAAAGAGTTGGACAAGCATTTGCGGAAACTCCTCACCC TTGGTAAAACCCAGGCAGGATGGAGGGAGCAGCTACTCTGCATTTGCTGCTCATCTGGGGCACACCACTC TCCTTGGACAGGGTGTGACCCCATGTTGTCCCCCAGACCCCTCTCCTCCTCACTTCTCTTCCTGTTCTGT CAAGTCTCATCTCCCTTTCTCCATCTCAGCTCTGTTTGGGGTTGACAGAAGTTTGAATGTCATGATACTC TATGTGTTATTTTCTGTCCTCATTGCTGTGTCCAAATGCTGAACAAGTAGCAATTTAAGGGAGCAACTGC TATGTGGCTCATAGCTTGAGAACATAGTCCATCAAGGTCGGGAAGGCATGGTGGTAGGACTATGAGGTGG CTCATGAGGCCAATGTTAGTGTTGTGGGCATGTGGGCAAGCTTTTGTCAACTTGACACAAACCTAAACAT CTCTGGGAGGAAGGATCTAAACTGAGGACTGTCCTCCATCAGGTTGTCCTGTAGGCTGGTCTGTGGGAGC ATTTTCTTGATTCATGGCTGATGTGGAAGAGTCCATCCCACTGTGGGTGGTGCCATCCACTAGCAGGTGG TCCTGGGTTATGTAAGAAAGCAGATAGAGTGAGCCATGATGAACAAGCCAGTAAAAAGCATTCCTCCATA GTTCTTGCCTCTGCTCCTCCTGGAGTTCACACTCTGTCTTTACTCAGTGATTGGAATGTGATATGGAAGT GTAAGCCAAATTAACCCTTTCCTCCCCAACTTGTTTTTCAGTCGTGATGTTTATCATGGCAAAAGAAAGG AGACCAGGACAGCAATTGGTAGTAGAGAGTGGGCTACTGTTGGGACAAGCCTGGCCATGCTGTTTTGGGG AGGATTGTAGAAGTGTTTGGAACCTCAGGCTGGAAAAGCCACCGAGTGCTCAAAGCTTAGTGGGCTTTAC TGCAAGAGCTGGACAGTTAGTGTTGAGGGAAACACAGTTGATGGAGGGCTGGCTTGTGGATTTTCAGAGG GAAGCAAAGACTCTCTGGGTCGTTTGAGTTTTTCCCCCCCAGTCTATAGTTTCTGGTCAGCTGGGACTGA CAATTCCACTTGATAAGAGAGCATTGTCATTGCAACAGAATCTTCTGGGAGGAGGTATTCCTTGGAATCA AAAGCTGTGGTCTGGTGGGGAGGCAGCCAGGCTGTCCATTAGGCTGGTGGCAAACCTGGGCAAACCAGGA AGTCAGGCAGCAGATAGATGCTGTTGCTTAGGTAAGCCTCTCTGCAAATGTCCTAAGGCACAATGAAAGG CGTGTCTCCTAGGTGATTCTAAAAGCAGGCAAGCTGACAGTGAAGATCCACCACGATACTGCTACTTCTT TGGCCTCTGATGCTGGAAGCCTTTGGAACGTTGTCATTAGTCCTGTTCTCCTCACCCTGGTTTCACCTGT TCTCATGAACTCAGCTTCTACCAGTTCACTTGTTCTACCATTCTAGGGTTAGGACTCCCCTTCCATACTT AGTTCACCACTGACCCTTTGATGGTCAGATTTCCTGCTCCAGGAGGCTAATCCATTCTTCATCCTACATC AGTAGTAAAGCCCAGGTGAGGGAATAAGGTGAGGGAAGCCCTGTCTGAGTGTGGACTTGCTGAAGGTGGC ATAAGAAGCCCCAGGCTCTATAGCTGCCAGGCTCATTGGGCCTTTGTGACAATGGGAAATAAGCTGGATT TGACCTGACCTACAGAGGCTGAAGAGGGACAACCAGTCAAGGCCAGGCTCAGTTACACAAGTCACAAGAC AAGAGGAAGCAAGGTGACTGAGGGAGGAACTGTTTGTGGAAACATTGTGCAGTTGGCAAGGAGTGATGCT TTTTTAGTGGGCAAGGTGTATGTGTGTCCATGGCTGGGTATACTGCCTGGTTGAGATATGTGTCCATGGT TGGGTATACTGCCTGGTTGAGATATGTGTCCATGGTTGGGTATGTTGTCTGGGTTGGGTATGTGACCATG GTGGGGTATGCTGTCTGGGTTGGGTATGTGTCCATGGTGAAGTATGCTGCAGTAGACAGAAATACTGCTT TGAGACTTCTCAAACAGAGCTGCCAAGCTCAGCAAAGGAAACTTAAGACATTCCATTATATGTGAACTTA GACAGTATCCATGGATAATCTCCTTATACAAGTTTTTCCCATAATAGACTTCATCTGGAATTTGATTCTG ATTATACATACTTTGTGTCTTACCTGGCTGTTTTCTTTATAAGGAGACTTGAAAGCAAGGCCAGGACTAA GGTGAGCCACTGATGGTGGTCAGTCACCAGGACAAGGGTGAGCCACTGACGGTGGTCAGTCACCTCTGAT AATCTCAATGATAAAATAAGTTGTAACTTAATACAATATTTAAAAAGCAACTAGAGTCCACAGGCTACTT CTAAGGATAGGATGTTGGCACCAGGGCAGGGAAGAGTGTGCAGAAGATGAAGCCCCAGAATGCCGGCATA GATTTCAACCATGCCCTTGTCTGCAATGCTGATAATGTGTTCCTTTTATCAATGATTTTGGTGTTGAACG ACTGCATTAAAACATTATTCATTAAAGTATTTCGTATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5363416 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5363416 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10709 same embryo
	EMAGE:10712 same embryo
	EMAGE:10714 same embryo
	EMAGE:10711 same embryo
	EMAGE:10713 same embryo
	EurExpress:euxassay_003193 same experiment
	MGI:4825078 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source