EMAGE mouse gene expression spatial database

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EMAGE:10713

Spata2 spermatogenesis associated 2 ( MGI:2146885)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003229. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003229_01	euxassay_003229_02	euxassay_003229_03	euxassay_003229_04

euxassay_003229_05	euxassay_003229_06	euxassay_003229_07	euxassay_003229_08	euxassay_003229_09

euxassay_003229_10	euxassay_003229_11	euxassay_003229_12	euxassay_003229_13	euxassay_003229_14

euxassay_003229_15	euxassay_003229_16	euxassay_003229_17	euxassay_003229_18	euxassay_003229_19

euxassay_003229_20	euxassay_003229_21	euxassay_003229_22	euxassay_003229_23	euxassay_003229_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10713_wholemount_strong.wlz

10713_wholemount_moderate.wlz

10713_wholemount_weak.wlz

10713_wholemount_possible.wlz

10713_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10713_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5366
Entity Detected:	Spata2, spermatogenesis associated 2 ( MGI:2146885)
Sequence:	sense strand is shown >T5366 GCTTTTGCCCCCGGTACTGTTCTCGGTTCCCGGAGCCGGCGGGAGCGGCCGGGCCGTGTGGGCCTGTGCT TTGGAGGCGCTCGCTTTCCTGGCAGGCAGCGGTGGAGCATGCGCTGGTCCATGGGCACCAGGCCAGTTCT TCATGTGATGTGCCAGCCTCCAGTTCCAGAGCCATGGGGGAGATGAGGTAGCCCTTGGGTTTGATTTGGG GAAGCCCAGTTCAATGGATACGAAGTACAAGGATGACTTATTCCGGAAGTACGTGCAGTTCCATGAGGGC AAAGTGGACACCACCCCCGGCAACCAGCAGCCTGGCAGCGATGAGTACCTGCGCGTGGCAGCTGCCACCC TGCTCAGCCTGCACAAGGTGGACCCTTTATATCGATTTCGGCTGATCCAGTTTTATGAGGTGGTGGAGAG CTCCCTCCGCTCGCTGAGCAGCTCCAGCCTGAGTGCTCTGCACTGCGCCTTCAGCATGCTGGAGACAATG GCCATCAACCTCTTCCTGTTCCCCTGGAAGAAGGAGTTCCGCAGCATCAAGACCTACACTGGCCCTTTTG TTTACTATGTCAAGTCCACGTTGCTGGAGAAGGACATCCGAGCCATTCTAAGGTTCATGGGCTACGAGCC TGAGTTGGGGACTGTGTACAAACTCAAAGAGCTTGTGGAGTCCCTCCAGGTGAAGATGGTCTCCTTTGAG CTCTTCCTGGCCAAGGTCGAGTGTGAGCAGATGCTGGGCATCCACTCGCAGGTGAAGGACAAGGGCTACT CAGAGCTGGACGTGGTGGCTGAGCGCAAGGGCAGCACGGAGGATGCGCGCGGGTGCTCAGATGCTCTGCG GAGGCGGGCTGAGAGTCGGGAGCACCTGACCACTTCCATGGCTCGTGTGGCACTCCAGAAATCAGCCAGT GAGCGAGCAGCCAAGGACTACTACAAGCCCCGGGTGACCAAACCCTCCAGGTCTGTGGATGCCTACGACA GCTACTGGGAGAGCAGGAAGCCCCCTTCAAAGGCCTCACTGAGTCTGCGCAAGGAGCCCCTGGCCATGGA TGTAGGGGAAGACTTGAAGGACGAGATCATCCGCCCATCCCCCTCATTGCTGGCCATGTCCAGCTCCCCC CATGGTAGCCCTGATGACCTTTCCTCCATCTCCTCCATCAATGGCCTTGGCCTTCTTCGTAGTACATACT TTTCCACTCAGGATGACGTGGACCTGTATACGGATTCAGAACCCAGGGCCACCTACCGGAGGCAGGATGC TCTGCGGCCCGATGTGTGGCTGGTCAAAAATGATACCCACCCCATCTACCACAAGCGTTCACCCCCCACC AAAGAGTCTGCCCTCTCCAAGTGCCAAAACTGCGGCCTGTCCTGCAGCTCCTCCCTCTGCCAGCGCTGTG ACAGTGTGCTGGTCTGTCCTTCGGCCTCCAAGCCCAGCGCTTTCCCCAGCAAGGCTTCTGTACACGACAG CTTGGCCCACGGGGCACCTATGCGGGAGAAGTATGTGGGCCACCAGACTCAGGGCCTTGACCGGCTGGCA CCTGTCCACTCAAAGCCCAAGCCCTCTACCACAGCCACCTCCCGCTGTGGCTTCTGTAACCGTGCAGGTG CCACCAACACGTGCACCCAGTGTTCAAAAGTTTCCTGTGACGCCTGCCTCGGCGCCTACCACTACGACCC GTGCTGCAGAAAGAGTGAGCTGCACAAGTTCCTGCCCAACAGTCAGCTGAACTACAAGTCCGCCCCGTTC TCCCAGCTCGTGTACAGATAGGCCTCACCCCCCGTCTTCCGCTACAAGGGCTACACACCACTGACCTGGC TTGGTCCCATCAAGAGGGAACAGAGCTGCACCGGAAGGAAGCAGAGACTTGAGCTTGACCGTGGAAGCAC CAGGGTTACTGCTTTGTCACATGGCTCCCGTAGCAGGTCACATTCCAGCCAGTGGCTGCTGTGTGGTCCG AAGGACAGGATGGCACTTCCATTCAGTCACTTCGCTGCCCTCTCCACTTCCTGCTCAGCTGGTATGGGTT TGGGTTCTGGTTCTGGTTCCCTTGAAATAAGTCTCTGTCTCTGGGACTCTTGCCACACCTGCCCCACCCA CTGCAAAGCCAGCTTGGACTGGGAAGGAGCCTTTGGGGTCACCTTCAAATCCCCACCAGGAGTGGTGAGC TAGAGGCCTGCTGGCTTGTGAAATGAGCCCTCCTGCCACACCGGGCCTCTCTGGTGGCTCGCCTCTTTTC CTGCTCCCTTGATACAAGGATCCCTAGGCAGATGTCCCCACCCCTTGTCTGCCCCCTGACTGGTATTAAT GGGTAGCCAAGTCCAGTATTAGTCGAATGCCATCTTGCCAGCTCCACTCAACCAGCCCACAATGTTGGCA AGCTCTGCTTGGCAGTTGGAAAGTGGACTCCATAGCAGGACTGACGTCCCCTCCTGTTTGCATAACCCCC TTACTGTATTGTACATACAGAATTTGAGGTTTATTTTTTAATGAGTGTTCAACTTGCTGTGTGTTTCAAC ATGGTGAAAGAAATTAATGATACGGTATTTATAAAGGTGCCCTGGGTTCTTTAGTCTTCCAAGGGGGGCC GCATGCCCTTTCCCCGTGTTTCTTCCACAGCTAGGGTGGAGCTGGGAGCCTGGCGTAAGGGACTGATAAA GCCCATGTTCCTTCCCATGGTGGTAGAGAGCTACTGTCTTCCCTACGGTAAATATTATACACCTACCTCC AGTGTGCACACAGGGGTTAGCATGGTGCCGGGGCAATGACCTCAGTGACAAGATGGGGACCTCAGCCCCC ACGTCTATAGCTCTGTCTGGTCTTGGTGCCTGCTGAGCATGAATCTTGTACCCTGAACCTTGAATCTCAG AAGGAAGTTCAGGCGGTGGGTGCCTCCACCCCCATCTAGCCTTCCTCACTGGTTCCCGGGGCTGTCAGCG CGGCTCTCAGTCTTTTTGCACTAGGGCGGGCAGAGGTGGGATGCGCTCCTTAGATGCCTCTCCAGTATTC AGTGTCCACACTTCCTGGGATAAATCCCACCTCTGGGTTTTCCTTTTTGGGGAGAGGGGCAGGGCAGGAA TTTGAAGCTGTCACTTCCTTGTGAAGTGTGAACACAGTTTAGCCATAGGCAGTTGTGATGTCCAGTGTTT GGGATCTATGGGCAAGTAGTTTTCCCAAAGCAGGCATTCAGTTCTGGAGCAGGCTTGACTCTGGCCAAAC CCTGAGCCACTGTGAATTTGCTGGTGGTCTTCCGACTTACTGGAGTGATTTTTTAGGTTTTCCAAATCTT ATATGACTCTTTTTTTATCTCTAGCTGATAGACCGCATGTAATTAGGTGTACATACAGCAGACTCTGGGC AGTTTTATTCCTGAGACTGGCCTTCCTGGGCAGCTCTTTGATGTCAGCACCTCAGACCTGGCAAGGCCTG GCCCTTTCCTGAGAGCTCCAGCTCTGCATTCCAGTCACTCCAGCATTGCAGGGACTCTCTTAAGATGGAG GACAGGCCACCTGAGGAAGTCCAGTGTGTTCCACCAGGAAATACAGTGTACAGACTGGCAAGGCCTCTCC CCTCCTAGGTGTGTCTCACTATAGGAGATCGCTGTGATCCGGGTAGTTGAGTATATGTTAATTTAAAATG CACATATTTTGCATTTGATTTCTACGGAGTTGAAATGTTGAGAGATTTAAAAAAAAAAAATAAAGTGTCT TCCATTGTTAAATGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5363513 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5363513 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10710 same embryo
	EMAGE:10709 same embryo
	EMAGE:10712 same embryo
	EMAGE:10714 same embryo
	EMAGE:10711 same embryo
	EurExpress:euxassay_003229 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source