EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10750

Pnrc2 proline-rich nuclear receptor coactivator 2 ( MGI:106512)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002876. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002876_01	euxassay_002876_02	euxassay_002876_03	euxassay_002876_04

euxassay_002876_05	euxassay_002876_06	euxassay_002876_07	euxassay_002876_08	euxassay_002876_09

euxassay_002876_10	euxassay_002876_11	euxassay_002876_12	euxassay_002876_13	euxassay_002876_14

euxassay_002876_15	euxassay_002876_16	euxassay_002876_17	euxassay_002876_18	euxassay_002876_19

euxassay_002876_20	euxassay_002876_21	euxassay_002876_22	euxassay_002876_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10750_wholemount_strong.wlz

10750_wholemount_moderate.wlz

10750_wholemount_weak.wlz

10750_wholemount_possible.wlz

10750_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10750_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 12 13 14 15

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 11 12 16 17 18

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 15 16 17 18 19 20 21 22

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T190
Entity Detected:	Pnrc2, proline-rich nuclear receptor coactivator 2 ( MGI:106512)
Sequence:	sense strand is shown >T190 GCAGCAGCATGGCAGGCCATGCAAAATGGGGGAAAGACCAAGAGCCTTTCTAACAACTCCAACTGGAATG CTGGTTTATCAAGTCCTAGCTTGCTTTTTAAGTCTCAAGCTAGTCAGAACTATGCTGGAGCCAAATTTAG TGAACCACCATCACCAAGTGTTCTCCCCAAGCCACCAAGCCACTGGGTTCATGTTTCCTTGAACCCTTCA GATAAGGAAACGATGACATTTCAACTTAAAACCTTACTTAAGGTACAGGTATAAAGTTAGTAGGTGTTAA CTTTAAATTGTGACGCTTACACATGACATCTGATTTATGTGCTGTACACACTATACAGTGTCGATGCAAT TTCAACAATTTAAGTGACTAATTGATTCTACTTGGTAAATTCAAGTTTAGATCTGGGAAATAGTAACCTT AACTTTTTTATTGTTTGATGGGAAAAATTAAGATTTATGGGTTAACACACAACTTCTGAAGAAG
Notes:	The probe template was PCR amplified from IMAGE:2647517 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2647517 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10748 same embryo
	EMAGE:10747 same embryo
	EMAGE:10745 same embryo
	EMAGE:10749 same embryo
	EMAGE:10746 same embryo
	EurExpress:euxassay_002876 same experiment
	MGI:4827307 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source