EMAGE mouse gene expression spatial database

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EMAGE:10761

Rab13 RAB13, member RAS oncogene family ( MGI:1927232)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003494. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003494_01	euxassay_003494_02	euxassay_003494_03	euxassay_003494_04

euxassay_003494_05	euxassay_003494_06	euxassay_003494_07	euxassay_003494_08	euxassay_003494_09

euxassay_003494_10	euxassay_003494_11	euxassay_003494_12	euxassay_003494_13	euxassay_003494_14

euxassay_003494_15	euxassay_003494_16	euxassay_003494_17	euxassay_003494_18	euxassay_003494_19

euxassay_003494_20	euxassay_003494_21	euxassay_003494_22	euxassay_003494_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10761_wholemount_strong.wlz

10761_wholemount_moderate.wlz

10761_wholemount_weak.wlz

10761_wholemount_possible.wlz

10761_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10761_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon meninges

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 not examined expression: see section 20

telencephalon meninges

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 19 20 21 22 23

olfactory lobe

moderate

regional

moderate expression: see section 10

hindbrain meninges

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 20 21 22

metencephalon

moderate

regional

moderate expression: see section 19

midbrain meninges

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 not examined expression: see section 20

spinal cord meninges

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6797
Entity Detected:	Rab13, RAB13, member RAS oncogene family ( MGI:1927232)
Sequence:	sense strand is shown >T6797 GAAAACTTCCTGGCTGGGACTCCGGCGGTTTCTGCCTGCCAACCCTCGGATCCCGCCTACCAGTGTTGGC TCTTCCCGACCCCTCCCCCGGCGCCCCCAGTGTCCGCCATGGCCAAAGCCTACGACCACCTCTTCAAGTT GCTGCTCATCGGGGACTCGGGGGTGGGCAAGACTTGTCTGATCATTCGCTTTGCAGAGGACAACTTCAAC AGCACTTACATCTCTACCATCGGAATTGATTTCAAGATCCGAACCGTGGACATAGAGGGGAAGAGGATCA AACTGCAAGTGTGGGACACGGCTGGCCAAGAACGATTCAAGACAATAACTACCGCCTATTACCGTGGAGC CATGGGCATTATCCTCGTATATGACATCACAGATGAGAAATCCTTCGAGAATATTCAGAACTGGATGAAA AGCATCAAAGAGAATGCCTCTGCGGGAGTGGAGCGCCTCCTGCTGGGAAACAAGTGTGACATGGAGGCCA AGCGGCAGGTGCAGAGAGAGCAGGCGGAGAAGTTGGCTCGAGAGCACAGAATCCGATTTTTTGAGACGAG TGCCAAATCCAGTGTGAATGTGGATGAGGCTTTCAGTTCCCTGGCCCGTGACATCTTGCTCAAGACAGGA GGCCGGAGATCGGGAACCAACAGTAAGCCCTCAAGCACTGGCCTGAAAACATCTGACAAGAAGAAGAACA AGTGCTTGTTAGGCTGAGAGCATTTCTTGCCTCCTATTCACCCTGAACCTGGAGGCTAGACCTGAGGGAG TCGGACTGAGGGATTGCAGATGGGAGAACTGTGGTGGCACCTCAAGGGGAGATGAGGGGAATAAGGAGAC CGGCGAGGACGAGACGGAAGAAAGGGGCAGGGAAAGGAGGGGGAGGAACCAAGGATGTGAAAGGTGAACA GAAGGGATTTGAGAAGAGGAAAGGAAGAAGAAATGAATGGCTCAGGCCTTGGACAGTCCAACATTAAAGT CAACATGCTGATCTCTCCATTCCTGGTTCAGGGTTAGGGTCCTGAGAGGCTGGCTCGGCACTACTCCGAG GGTCCCTCACTCTACAAGGTCTTTGTTAGTATTAAAGGCCACTGTTTTGCATGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3709140 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3709140 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10762 same embryo
	EMAGE:10764 same embryo
	EMAGE:10763 same embryo
	EurExpress:euxassay_003494 same experiment
	MGI:4827565 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source