EMAGE mouse gene expression spatial database

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EMAGE:10762

Acsm1 acyl-CoA synthetase medium-chain family member 1 ( MGI:2152200)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001267. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001267_01	euxassay_001267_02	euxassay_001267_03	euxassay_001267_04

euxassay_001267_05	euxassay_001267_06	euxassay_001267_07	euxassay_001267_08	euxassay_001267_09

euxassay_001267_10	euxassay_001267_11	euxassay_001267_12	euxassay_001267_13	euxassay_001267_14

euxassay_001267_15	euxassay_001267_16	euxassay_001267_17	euxassay_001267_18	euxassay_001267_19

euxassay_001267_20	euxassay_001267_21	euxassay_001267_22	euxassay_001267_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10762_wholemount_strong.wlz

10762_wholemount_moderate.wlz

10762_wholemount_weak.wlz

10762_wholemount_possible.wlz

10762_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10762_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

left lung lobar bronchus

strong

regional

strong expression: see section 17 weak expression: see section 06 07 08 09 10 14 15 16

right lung middle lobe lobar bronchus

strong

regional

strong expression: see section 17 weak expression: see section 06 07 08 09 10 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4913
Entity Detected:	Acsm1, acyl-CoA synthetase medium-chain family member 1 ( MGI:2152200)
Sequence:	sense strand is shown >T4913 CTTACAAGCCGTCTCTCCTGGCTATAGTCTACCTGTGTCCTTCACAGTACCAGGACTGCCCTAATATGCA GTGGCTGAAGAGTTTCCAGATCTGCAAAGTCTTACAGGGCTTCTCCCTAAGCCCCACCCAGCTGCACAGA CGGTTGTTTTCAAGAGTTGGAGCTCCAAGATGGAATGACCATGATTCACCTGAGGAGTTTAACTTTGCAA GTGATGTCCTGGACTACTGGGCTCAAATGGAGGAGGAGGGCAAGAGAGGACCAAGTCCAGCCTTTTGGTG GGTGAATGGCCAAGGAGATGAAATAAAGTGGAGCTTCAGGAAGCTGAGGGACCTCACCTGTCGCACTGCC AACGTCTTTGAGCAGATTTGTGGCCTGCAGCAAGGAGATCACCTGGCCTTGATTCTGCCCCGAGTGCCCG AGTGGTGGTTGGTGACAGTGGGCTGCATGCGAACAGGGATCATCTTCATGCCTGGGACTACCCAACTGAA AGCCAAGGACATTCTCTACCGAATACAAATATCTCGAGCCAAAGCCATTGTGACCACAGCTAGCCTTGTC CCAGAGGTGGAATCTGTGGCTTCTGAGTGTCCTGATCTGAAAACCAAGCTGGTGGTGTCTGATCACAGCC ATGAAGGGTGGCTTGATTTCTGTTCACTGATTAAATCAGCATCCCCAGACCATACTTGTATTAAGTCAAA GATGAAGGATCCCATGGCCATCTTCTTCACCAGTGGGACCACAGGCTACCCCAAGATGGCAAAGCACAAC CAGGGACTTGCCTTCCGGTCATATATCCCTTCATGCAGAAAATTATTGAAGCTGAAGACATCTGACATCT TGTGGTGCATGTCAGACCCAGGATGGATTCTGGCTACCGTGGGGTGCCTGATCGAGCCATGGACATCAGG ATGTACAGTCTTCATCCACCACCTCCCTCAATTCGACCCCAAAGTCATTGTAGAGGTACTGTTCAAATAC CCCATCACTCAGTGCCTTGCTGCCCCAGGCGTGTATCGAATGGTTCTTCAGCAGAAAACCTCCAACCTCA GGTTCCCCACCCTTGAGCATTGCACTACTGGTGGGGAGAGCCTGCTGCCTGAGGAGTATGAGCAGTGGAA GCAAAGGACAGGTCTTTCCATCCACGAGGTCTATGGACAGTCAGAAACGGGGATCAGCAGTGCCACCCTC CGGGAAATGAAGATCAAGCGAGGCTCCATAGGGAAGGCCATCTTACCCTTTGACTTGCAGATCATCGATG AAAAGGGCAACATCCTCCCACCCAACACTGAAGGATACATTGGCATCAGGATCAAGCCCACCAGGCCTCT AGGCCTCTTCATGGAATATGAGAATAGCCCAGAGAGCACATCTGAAGTGGAGTGTGGGGACTTTTACAAT AGTGGGGATAGAGCGACCATTGATGAAGAGGGCTACATCTGGTTCTTGGGAAGGGGCGATGATGTCATCA ATGCTTCCGGGTATCGCATCGGGCCTGTAGAGGTGGAGAACGCCTTGGCGGAGCACCCAGCAGTGGCAGA GTCTGCGGTGGTGAGCAGCCCGGACAAGGATCGAGGAGAGGTGGTGAAGGCGTTTATTGTCCTCAACCCA GAGTTCCTGTCACACGATCAGGAACAGCTTATCAAAGAGCTACAGCATCATGTGAAGTCAGTGACGGCAC CATACAAGTACCCCAGGAAGGTGGAGTTTGTTTCAGAATTGCCCAAAACTGTCACAGGCAAAATCAAAAG GAAGGAACTTCGAAACAAGGAGTTTGGTCAGCTATGAACAGCAATCAACCCAGAGCATCCTGTGACCTGA ATCCTTGGCTGTTGGGGTCAGGGTGGTGTGGGATGACGATGGAATGCAGGAAGGGTAAATGTGGAAGAGA AAACCAAGACCCCAACATTTTTGCTTTCTTCAAACTCAGTTGGTCCCCTTCCTGGATACCTTCTTCTTCC CCTTCCACAGTGCACTCTCAGACTGTTTGTGGACAATAAAAATACTGACTTGAATAGCAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:5102652 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5102652 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10764 same embryo
	EMAGE:10763 same embryo
	EMAGE:10761 same embryo
	EurExpress:euxassay_001267 same experiment
	MGI:4822948 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source