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EMAGE:10774

Serinc1 serine incorporator 1 ( MGI:1926228)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10774 EMAGE:10774 EMAGE:10774 EMAGE:10774 EMAGE:10774
"Pseudo-wholemount" of euxassay_001094. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001094_01 euxassay_001094_02 euxassay_001094_03 euxassay_001094_04
EMAGE:10774 EMAGE:10774 EMAGE:10774 EMAGE:10774 EMAGE:10774
euxassay_001094_05 euxassay_001094_06 euxassay_001094_07 euxassay_001094_08 euxassay_001094_09
EMAGE:10774 EMAGE:10774 EMAGE:10774 EMAGE:10774 EMAGE:10774
euxassay_001094_10 euxassay_001094_11 euxassay_001094_12 euxassay_001094_13 euxassay_001094_14
EMAGE:10774 EMAGE:10774 EMAGE:10774 EMAGE:10774 EMAGE:10774
euxassay_001094_15 euxassay_001094_16 euxassay_001094_17 euxassay_001094_18 euxassay_001094_19
EMAGE:10774 EMAGE:10774 EMAGE:10774 EMAGE:10774 EMAGE:10774
euxassay_001094_20 euxassay_001094_21 euxassay_001094_22 euxassay_001094_23 euxassay_001094_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10774Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10774_wholemount_strong.wlz
10774_wholemount_moderate.wlz
10774_wholemount_weak.wlz
10774_wholemount_possible.wlz
10774_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10774_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
facial vii ganglion
moderate moderate
homogeneousmoderate expression: see section 04 05 20 21
glossopharyngeal ix ganglion
moderate moderate
homogeneousmoderate expression: see section 06 07 18 19
trigeminal v ganglion
moderate moderate
homogeneousmoderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22 23
vagus x ganglion
moderate moderate
homogeneousmoderate expression: see section 08 09 16 17
vestibulocochlear viii ganglion
moderate moderate
homogeneousmoderate expression: see section 05 06 07 08 17 18 19
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4301
Entity Detected:Serinc1, serine incorporator 1 ( MGI:1926228)
Sequence:sense strand is shown

>T4301
CGCCGAGCTGGTGACGCGGCGGCGATGGGGAGCGTTCTGGGGCTGTGCTCCGTGGCGAGCTGGATTCCGT
GTTTGTGTGGCAGTGCCCCGTGTTTGCTGTGCCGATGCTGCCCCAGTGGGAACAACTCCACTGTGACTAG
ATTGATCTACGCACTTTTCTTGCTCGTCGGAGTATGTGTAGCTTGTGTAATGTTAATACCAGGAATGGAA
GAACAACTGAATAAGATTCCTGGGTTCTGTGAGAATGAGAAAGGTGTTGTTCCCTGTAATATCCTGGTTG
GCTACAAAGCTGTGTATCGCCTGTGCTTTGGTTTGGCCATGTTCTATCTTCTCCTCTCTTTACTAATGAT
CAAGGTAAAAAGCAGCAGTGACCCTAGAGCGGCAGTCCACAATGGATTCTGGTTCTTTAAATTTGCTACA
GCAGTTGCAATTATCATTGGCGCATTCTTCATCCCAGAAGGCACTTTTACAACTGTGTGGTTTTATGTAG
GCATGGCGGGCGCCTTTTGTTTCATCCTTATACAACTAGTCTTACTTATTGATTTTGCCCATTCTTGGAA
TGAATCGTGGGTTGAAAAAATGGAAGAAGGGAACTCAAGATGTTGGTATGCAGCCCTACTGTCAGCTACA
GCTCTGAATTACTTGCTGTCTTTAGTTGCCGTCGTGCTATTCTTTGTCTACTACACTCACCCAGCCAGCT
GTGCAGAGAACAAGGCGTTCATCAGTGTCAACATGCTCCTCTGCATTGGCGCCTCAGTAATGTCCATTCT
GCCCAAGATCCAGGAATCACAACCAAGGTCTGGCTTGTTACAGTCGTCAGTAATTACAGTCTACACAATG
TATTTGACATGGTCTGCTATGACCAATGAACCAGAAACAAATTGCAATCCAAGTCTACTGAGCATCATTG
GCTTCAATACCACAAGGCCTATTCCAAAGGACGGGCAGTCTGTTCAGTGGTGGCATCCTCAAGGAATTAT
AGGACTTGTTCTTTTCCTGCTGTGTGTGTTTTATTCGAGCATCCGTACCTCCAACAACAGTCAGGTTAAT
AAGCTGACTCTAACAAGTGATGAGTCAACATTGATAGAGGATGGGAATGGCAGAAGTGACGGATCACTAG
ATGATGGTGATGGCATCCACCGAGCTGTAGATAACGAGAGGGATGGAGTCACGTACAGTTACTCCTTCTT
TCACTTCATGCTCTTCTTGGCTTCACTTTACATCATGATGACCCTCACCAATTGGTACAGGTATGAGCCT
TCTCGTGAGATGAAAAGTCAGTGGACAGCAGTCTGGGTGAAAATCTCTTCCAGTTGGATTGGCCTTGTGC
TGTACGTGTGGACACTAGTGGCACCACTTGTTCTTACAAATCGGGATTTTGACTGAATGGGATGTCTGGC
ATGAGTCCCACTTTGGCCATTGTCTATTTGACAGTAACAGTATTCCCAATTTTTGTAAAGTTGTATGTGC
TGTTGTGTAAGTGTAACACTGTTTTTCTCTTGCATGAAATAGATTTACTCTGCCAATTTTATCCATGTTC
TTGCTAAGTCTATTGATGATATGAATTGGAAAGGGAGAATTTTGAATATGGTGAGAAATTGGGAAAAATG
GACATTGATAGGCATATCATCTGCTTTGTGTACATATGGTATTAAAACTGAAAACAAATATTCTTGACAT
TTGGGTTTCAAAATTGTGTTAGCACTTATTTTAGAAACATTAATTAAGTGTATGGCTGCCTTTGAAATAC
TTGATGTGTTGTTGTTGCCTTACAGAGGACTACAAGTATAAAGCAAATAGCTGTTTTAAAATTATGTAAA
TAAGTCACAGGCTAGTTATTTGAAAACTCTAAGTCACTTTAATTTTGTCAGAATCTTCTCTTACTAGGTA
GGCCCAGGATGTTCCATAGTTAGTGGAAAACAGTACAGGAGGTGGAGGGAAGCTAAGCAATAGACAGGAG
CCGTCACAGGAAGTCACTGGCAATACGGGTTTAAAGCAGGTACTTAGATCACGGTTTTCTCTGTAAGAAA
ATATGGCCGTCACCCCCGCCCACCAAAAAAAAATGATTTGCTCTTTGTGAACACACAATTATGAATTTCA
TGGCGATGTTAACAATTCTGCATTTAGACAAGGTTTTGCAAAAGATATTGTGTTAGAAAAATTTGAAACT
AAAAGCTTCCCAAACAAAATGGGAAACCTTACAGGACATAAAACATTAGGCTCGGGTTAGGCACTAAGAT
AAAGTCTAGTCCAGTTCTCATTTTAAAAGTCAACTAGCTCTAAAGCTAGTTAGTGACAAAGTTGGATACC
AAAAATAGCCAACACTATGACATAAAAACTTTGTGTTCAAAATGTACAATTCACTTTATACATTATTAAT
AGTAGATAAATTTGTGTAAAATGTATCGCTGCTGTCCAGCATGCTTTTCTTCTTTTTATCCAAACATGAG
GCCAGGAGGATAATTAACTTGAGGAATAGACAACTTACATTCACTACATATTTAATCATGCTTAAAATGT
TTGTATTTACTGCCATGCAATTGATATAGTAAATCGACTAGAAAATTAGTATGCCACTTAATTAAGATAA
AAATGCTTAGTTATTTATGTTAGTATCTAGCTTTTAAAGAACTACATGTGTCTTTAAAAGTATTTATTGT
GCAAATGTACTATAGCAGTTCATCTTCATGTTCCAGCCTTACTCTGTGTTAATACTGCTTCACAATTCTG
AATGGCTGTGTTCTCTTAAATAAATGCATTCAAAGAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4506673 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4506673 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10772 same embryo
 EMAGE:10771 same embryo
 EMAGE:10773 same embryo
 EurExpress:euxassay_001094 same experiment
 MGI:4827968 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS