EMAGE mouse gene expression spatial database

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EMAGE:10795

BC030476 cDNA sequence BC030476 ( MGI:2447772)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003211. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003211_01	euxassay_003211_02	euxassay_003211_03	euxassay_003211_04

euxassay_003211_05	euxassay_003211_06	euxassay_003211_07	euxassay_003211_08	euxassay_003211_09

euxassay_003211_10	euxassay_003211_11	euxassay_003211_12	euxassay_003211_13	euxassay_003211_14

euxassay_003211_15	euxassay_003211_16	euxassay_003211_17	euxassay_003211_18	euxassay_003211_19

euxassay_003211_20	euxassay_003211_21	euxassay_003211_22	euxassay_003211_23	euxassay_003211_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10795_wholemount_strong.wlz

10795_wholemount_moderate.wlz

10795_wholemount_weak.wlz

10795_wholemount_possible.wlz

10795_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10795_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

moderate

regional

moderate expression: see section 11 12 13 16 17 18 19 weak expression: see section 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5424
Entity Detected:	BC030476, cDNA sequence BC030476 ( MGI:2447772)
Sequence:	sense strand is shown >T5424 CCACGCGTCCGATCTCATCTCCGGAGCTTTTCCCCATTTCCCTTCCCAGCGCCCTACGCCTCCTGTACAG CAGGTTCCTTGGTTTTAATTGCCCCTGCTCTCTCTTGCCTGCTGCTCCTCCAGCTGTCCGGCACTCTGTT TTCTCCGGCTTTATCCACCTGTGCGTCTCTCGATTGCCCCTCCAGGTGCGCACCCCGTAGCCAGAACTCC GCTAGGTGTGCCTGTGCCTTGAGCTCTTGGGAAAGCAAGATCAAATAGTTGGAGAAACTTCAGCCGCTAG GTTTGCGGTGAGCATCGCATCCACACCCGTGAGGGGCTGGGCCAGGTGACTGCTCTTTGAGCGATGGGCT GCTCCAGCAGCGCCCTCAACAAAGCGGGCGACAGCAGCAGGTTCGGCAGCGGAGTAACTTCGAATGAGAA TTCTTCCACTGTAGAACATAACAAGTTCTGTGTAGACCAACCTAAACCATGTACACCAGGAGGAGAAGCT GCCTTTCATGGCAACACACAAAGGGAAAGCCATCCTTCACTCGAGAGGCCCAAGGCTTCAGTGGTGCCTA CAGCAAATGGTGTTAAATCCTACCATCAACCATCTCTGGCAAATGATGAGACCCCTGGAAAGGAAGCCAC AGATCACTCAAGACCCACAAAGAAGATTGAGCCTCTAGTGCAAGGCGGGGAGTGTGAGCAACCTCAGCCA GGAGGCAAAGATGATATGTTGGGAACTGAGGAAGTGAAGAAAGATGTGGAAGCAAGGACAGAGGTCCCGT CTTTAAAAGGAGATGCTGAGATTAAGCCTCTGAGGCTGTCATCAGAGAGGGACTCCCCTGGAGCTCCACA GGCTGGGACGATGAAGTTTCTACAAACAGCCGAGAACATTCTGCCTCTGGAAACAACTCAGGAGCTACCA CCAAAAGAAGCAACGGGAAAGGGTGCACAGCCCCAGATTCTAGAAGCAATTCCCAAAGAGAACAGCTCTC CAGAAATAGAAGGGATCCAATCTGCAGAAAGCAGCGGACAGCAGCAACTTGTAGAAGCCCCAGGTGAAGC TGAGCAGCCCCAAGCTCTAGAAACAGTACTCAAAGAGAACGAAACATCACAGATGCCAGGCAGAAGCCAG CCCGTGCCAACACCTGTAATGAACAAGTCACCCTGTGAAGCTCCTGACGGTCTCAGAAATGCCCATGAAC CTCAAGTAACAGGTGGAAACAGGGTACAGCCAGCCGAAACTGGAGAGACAGCAGCAAAGGTGGAAATGGC CAGAGAAATCCACCCTGATAAAGAGGAACAACACATTGAAGGTGAGACAGGAGAAAAGGTGGAAGCAGAG ATGAAAAATGAGAAAGAAAGTGAAGAAGCTGAAACTAAAGAGAAAGAAACAGGAGAAGCTGTGGATCTTG GAGCAGCAGGGGCTGGTGACAGAAGGGCTTAGTGTGCAGTGTTACACCAGAGGGCAGGGATATTTGAGGC TGGGAGGGCGGGCACCACCTTTCTGCGGCTTTGTGGTTTATACAAGGAGTATAGTTTTAAAATGACTGTA GGAACCAAGAGAACAATGCTGTTTTTGTTTGTTTGTTTGTTTGTTTTCTTTTATTGTTTACACCATAAAA CTGCTAAGCCAGACACGGTTTTCTTAGATACTTAGGATAATTATGAATTCAAAGTGACAATAGCCATAGC CAATCAAAACTGTAAGATCATCTAAGAGAAAGAAGGCAATCTTCACCCTTGTAGCCATGAGGTCTCAGGA TAAAATGAGACCCTAAAAGGAAAGTACAAACCTTTAAATGTAGGTGTCTAGAGAGAGGAACTAGCAAACT TTAAGGAACTCATGATAAGAGAGTTTTGCAGTTATAATAGTGATGGAACAAAATTAAATAACCCCAGAAC ACAGGACCAACTCTACCTAATATTTCTGTCCTGTAATAGAAATTTGACTGAGCCGGGCGTGGTGGCGCAC GCCTTTAGTCCCAGCACTCGGGAGGCAGAGGCAGGAGGATTTCTGAGTTCGAGGACAGCCAGATCTGCAA AGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTTGAAAAACAAAACAGAAAGAAAGGAA CCTCAGGGAGCCTGTATTCTAACTACTTTCTGTCCTGAAATCTATCTACTACTACAAATCACTGTGACCC ATGGCATACTAAATGTTAACAAAAATTTCTGGAGGGATGCAATTGGGGTGCTGTTGCAAGCTTTGGGATT TTTTACAGTTAAAGAGTCCACATTTTCCATGTGCCATTTTGAATTGGCTGGTGATTTTATACTTAAGGGG GGGGAGTGTTGGTGATCTCTCTCTCTTTCTTTCTCTTTCATGTGATTTACTAGCAGTAAAATAATTTTAA ATCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5368170 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5368170 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10798 same embryo
	EMAGE:10796 same embryo
	EMAGE:10797 same embryo
	EMAGE:10799 same embryo
	EurExpress:euxassay_003211 same experiment
	MGI:4823422 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source