EMAGE mouse gene expression spatial database

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EMAGE:10799

Zfp740 zinc finger protein 740 ( MGI:1915994)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008242. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008242_01	euxassay_008242_02	euxassay_008242_03	euxassay_008242_04

euxassay_008242_05	euxassay_008242_06	euxassay_008242_07	euxassay_008242_08	euxassay_008242_09

euxassay_008242_10	euxassay_008242_11	euxassay_008242_12	euxassay_008242_13	euxassay_008242_14

euxassay_008242_15	euxassay_008242_16	euxassay_008242_17	euxassay_008242_18	euxassay_008242_19

euxassay_008242_20	euxassay_008242_21	euxassay_008242_22	euxassay_008242_23	euxassay_008242_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10799_wholemount_strong.wlz

10799_wholemount_moderate.wlz

10799_wholemount_weak.wlz

10799_wholemount_possible.wlz

10799_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10799_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5414
Entity Detected:	Zfp740, zinc finger protein 740 ( MGI:1915994)
Sequence:	sense strand is shown >T5414 CCACGCGTCCGCAAAGGGCCACCGAAAAGACAGTGATAAATCCCGCAACCGCAAAGAGGATGACAGCTTG GCTGAGGCCTCTCATTCAAAAAAGACTGTTAAAAAGGCAGGTAATGGGGAAGCCCCAGACTCGTGCAGTT TGGTAATGGGGATCTTCCCTGGTGAATCTGAGGCCACTCTGACAGTTGCCGTCCACTCTTGCTGAACATT CTTGGTGCAGGTGGGAAGCGTGGCTTATGTTCTTGGAGCGGGGCAGGTGAACCCCAGCTCACTCACGCTG TTTTGTTGGCTGTGGCAAGACAGCTGGTTCATCGACCCCATCTTCCTCCCTTCACGGCTCTTAGCTCAAG GGGTGTGACAGGTGACAGCACCGCCTTCCACAGTATCAGAAAGGTTCTCAGGTTCTCGGTCCGGCAGCTG TAATTGGTCCCAGAGTAGAATCGTCTCTGGGAAGAGGACAGAGACACCCACATGACATGCCTGATTGTTG CCCCCAGGTGGTGGTAGTGGAACAAAATGGCTCTTTTCAAGTAAAGATTCCCAAAAATTTTATTTGTGAA CACTGCTTTGGAGCCTTTCGGAGCAGTTACCACCTCAAGAGGCACGTCCTAATCCACACTGGTGAGAAAC CATTTGAGTGTGATGTCTGTGATATGCGTTTCATCCAGAAGTACCATCTCGAACGCCACAAGCGGGTACA CAGTGGCGAAAAGCCTTACCAGTGTGAACGATGTCATCAGTGTTTTTCTCGGACAGACCGATTACTCAGA CACAAACGGATGTGCCAAGGATGCCAGTCCAAGACTTCTGAAGGGCAGTTTTCTCTATAGGCACAAGGGG CCCTAGGTGGTGGGAGTGCTGAGGAGAGTCTCTGGAAGAGCATAGCCCTGTGTTCTGACGGTCCCACCAC CACTCCCTCTGAACCTGTCCCCCTTCCTAGAGGACCAGCCCCAGGTTAGCAGAAGAGCGTCTGAGGACAT CGGCTCCAAAGCCCCAACTCTGTAACCAGTCTGAGGCTATGAGTATCCCAGGGACACCTGCAGCATTCTT GGGCAAGGGAGCTGGTCCTTGAACCTCAGCTGAGATGAGGTGGGGACTCAAAGGTGCAGGACCAAGACTG AAATGTTCCCACAACCTTGGACTGGAACTGGCAGCTAAAATGGAAAGGACCGGGGAGGGGTTTTTGTTGG TTCTGCTTTTGTTTTTGTTTATCCAAAAGCAATTTAAACAGGTAAACCGGGGATACAGGTAATGTGGAGG GGACAGTCCTGAAGCACATCAAGGACTGTGGCTGGCCTGGCCATGTCTGTCACCACAGTCCCTCGGTGTT CCACAGCCCGCCTATTTGAAAGCAAATAGGAAGGGGGCTGGCAGCTGGGCTTCCTTGGACTTCTTATACA CTGTGAAGACAAAAGGAGAAACTGGGAAGGACAGACGTGGGCTGGACAAGAAGCGTAGGCATACCTTTTC TTATCTCCTCAAGAAGAGCGGTGTTGTGTCACGGGTGTTCCCATGGTTTGTGTGGCACTGGGGCCTGATG GGATCAGCTCAAGTGCCTTGTGGAGGACTTGGGTTCCGTAGCCCATTTGGCTCTTTTTTCTGGGAAGTTG GTCTTGGTTGTGGTTTGTGGCTACCTCGCTTGTCCTTGACTCTAGAACCCAGGCACAGTTGAGCAGGGGG TTGTGGGTATCAGAGTATGCTGTCCTTTTGTGTCCTCTTAGAACAAGGATAGTCCAAAGTTTTGTTAACT ATGAGAAAAACAGAAATTGGGACAGTCCTTATGGGACTTGTGTGATGGAGCCCTCTCCCTGTCAGAATCC TCTGCCTGTTCAAGTAGAGGAGATGGAGGTCTGGTCTGGTCTGGTCTTCAGTCTCAGAGAACCGACCTTG GCTCCCTCCTGATAGCATCCCTGCAGAGTGGGGGGGGGGGGGTGCTTCAGAGGAAGGATTGCAGGAAGAC ACCTAATGTCTGCTTTATTCTTGACCTTACTCTGTTATAACACCCTTCTATCTTGTCTACCCCTGGAAAG GTGATTGGATTAGTGTGTGTGTCTGATTTGTGAATGCTTGTGTTTCTTTGCTTGGAAGGGGTGAAAATTG TTGACTGAAATTCATTCGGCCACCAACTTGGAGGACAGAAAAACGTAGGGCAGCCTTCCCACCCAGACCT GAAGGACGTCTGCCTCCCTTGTTCTTGCTGAGAACAGGGGAACAGAGGGGCTTGTTTCAGAAGAATGTTG AGGGTTTTTTCTCATTGTTGTTAGGTTGATTTAACCGTGTACTGATCAGAGTGTTTCTAAGCCAGGGCTT CTGAACATGTACTTTCTAAAGTGAGATAACTTCTGCTAAAGAAAGGATTCTTAAGGGAGACCTTGGGGTT TCATTGAGCACTCCAGGACCCCTGAGGCCTACTTCAGGTGTAATCTTTGGGTTACACCTGAGCACACAAC TCCAGCTGTGGGTAGGAATCTGAGCTGGCCGCCTCCAGGAAGAAGTTTTGACTTGCAAATGAGAGCCAGG CAGCAACCTACCTTAAGACCTCTTCCAGTGTATTGGGAGATGGTCCACCACAACTTAAGGGGAGAAGGGA GAATGTCTCAGCCTTCAGTTTTCCTCTGATAGCACCTGAGAGGAGGTCTGAAGAGGACAGCCTGCCGTGT GTTCTGGCTCCCTGCTGTCCCTCTAACGTGCTGTAAAGCCTGCGCCTCTGACCTTCGTCCGCCGTGTGGC CCCTGGGCATCCATGTTCGTGATGCACAGACCCTTTGCTGTTAGAGGGTTCTGCTGGGCTGGGATTTTTC TGTCACTGGGAAAGAGGATACGCTGAGAAAGATATCCTCCCAGAAATGCTTTTGAAAGTGGAATCCCTCC CTGCCACTCGTAGATGATCTGTTCGTGCTGAAAGTTGGCAGAGTGGCCTGTGCCTCTGCAAGTATGAATG TGTTTCCTGGGTCAGAGCAGGCCCTGCAGACAGTGGCGTTCTGCCTAGGTGGGAGCTGAGAGAAGTGAGA GAGAACTGGATGTGTACAGACAGCCCACCCAGAACCACACTGCCAAGGCCAGGTGTGAGAGGCTCAGAAG CTGGCAGTGGGTAAGGACAGACAGGCTCTGGGGTGGAGAGTAATACAACAGCCAAGAGCCTGGGCAGAAG CCACATCTCTACCCTGGGGGAATGCACAGACAGCTGCAAAGGCTCCTGAGCCTGATGCCCCCCTCTGCTG GGGTGGGTTGTTAGCACTGGACAGATGTCTCAGGTGTGTGTAGAAGGGGAAATGAGAAGGTGGGTGCTCA GACATCAGAATGTGGATGTGGGTTTGGTAGGGAGAGTAATACCGGTCCTTGGATGGACTCCTGCACCAGG CCGTCCCATCCCACCCTTGCTGGTGCCTGCCCTTCCCATCCGTCCCTGACAGCCTCTTGTCATTTAGCTA CTGATTGTACTTCATGGCTTGACATAGAGGGTTAGAGAAGTGGGGTGACCACACTTTGAGCCCTCTCCCC AGGTTCCTTCTGCCCCTGCTGTGGCCCTGGGCTTTTATCTTCAATTCCCCTCCTACCGTGCTCCATTTTT TTTTAAATATACTTATTTTGCTATGGGGGAGGGGAACTATCAAATGGACAAGATTACTTTTAATGTAGTT TTTATAAGATGTTATAAACTTGTATCTTTTTACCATTAAAGTGCAGTGTATGTTCCTTCGTGACATATTT AGGATATTTAAATAAAAAGGATGGCGCTTTTCTACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5367159 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5367159 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10798 same embryo
	EMAGE:10795 same embryo
	EMAGE:10796 same embryo
	EMAGE:10797 same embryo
	EurExpress:euxassay_008242 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source