EMAGE mouse gene expression spatial database

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EMAGE:10832

Setmar SET domain and mariner transposase fusion gene ( MGI:1921979)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003065. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003065_01	euxassay_003065_02	euxassay_003065_03	euxassay_003065_04

euxassay_003065_05	euxassay_003065_06	euxassay_003065_07	euxassay_003065_08	euxassay_003065_09

euxassay_003065_10	euxassay_003065_11	euxassay_003065_12	euxassay_003065_13	euxassay_003065_14

euxassay_003065_15	euxassay_003065_16	euxassay_003065_17	euxassay_003065_18	euxassay_003065_19

euxassay_003065_20	euxassay_003065_21	euxassay_003065_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10832_wholemount_strong.wlz

10832_wholemount_moderate.wlz

10832_wholemount_weak.wlz

10832_wholemount_possible.wlz

10832_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10832_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4857
Entity Detected:	Setmar, SET domain and mariner transposase fusion gene ( MGI:1921979)
Sequence:	sense strand is shown >T4857 GGCGAGTAGTCGAAGCGAGACCATAAATGTCTGCGGAAGGTGTGGAGAAGCTTTCTCTCGAGATAGCAGC ATCTGAGGAGGAATCTGTAGCCCCGACAGAACAACAAGATGTAGCGTGCGGCCTGGAGAACTTGCCTGTG AGCTTGTGGCCTTTGGGGGCGGAGCCCAGGCCTAAACCTTTCCAGTATACTCCTGATCATGTCGCTGGAC CTGGAGCAGACATTGACCCCACACAAATAACCTTCCCTGGATGTGCTTGCATCGAAACTCCATGTGTCCC TGGCACTTGCTCTTGTCTTCGCCATGAGAATAACTACGATGACAATTTATGCTTTAGGGATGTGGGATCC GAAGGAAAGTACGCCAAGCCAGTTTTTGAATGCAATGTTCTGTGCCAGTGTGGCATGCGCTGCAGAAATA GGGTGGTCCAGAATGGCCTACACTTCCTTCTCCAGGTGTTCCAGACAGAGAAAAAAGGCTGGGGACTTCG GACTTTGGAATTTATACCCAAGGGAAGATTTGTCTGTGAGTATGCTGGGGAGGTGTTAGGATTCTCTGAA GTGCAAAGAAGAATTCACCTACAAACATCACATGACTCAAATTACATCATCGCCGTCAGAGAACACATTT ACAGTGGACAGATCATGGAAACATTTGTCGACCCTACCTACATAGGAAATATTGGGAGATTCCTCAACCA TTCTTGTGAACCAAATCTGTTGATGATTCCTGTCCGAATTGACTCAATGGTACCCAAGCTGGCACTTTTC GCAGCCAAAGATATTTTGCCAGGAGAAGAACTCTCTTACGACTATTCAGGAAGATTCCTTAACCAAGTAA GTAGGAAAGACAAAGAAAAGATAGACTGTAGCCCACCACGAAAGCCTTGTTACTGTGGGGCCCAGTCATG CACCACTTTCCTGCCCTATGACAGTTCACTATATATGGCCCCTTAGAAAATCCAGGCACCAGTTAGGAAA GGAGATCCTACCTGCCGGCCCCTTTGCAGACAGAAATCAGAGGGAGCCAAGCATTAAGTGATAAAATACC ATCTGGGTTCGTCTCCTACAAGCAAACTACTCTCAAGGGAAGGCTACCTCATAGTCCGCATCTTGTTCAT CCCTGTAAGACAAGGGGTTGCCCAGCCCTGCCAGGTTCCCACATTCAACTCAGCAATTTGGCCCAGATAG AATGTCCATCAATACACTCTCTCCCAGAATACTTGTCTTTGTAGCTAAGTGTGGGTAAGTGGATATGTTG TAACTAACAGGATACAAGCAAACAGGATGAATCCTTGGGCATCGTCCTTGTATTTCTTCTTGGAAACCAG AAGTCAGACCTGATGGCTGGAGTTGGAACAGCCATCCTAAAGCCAGGACCACCCTGGCAAAGGGTCATTC AGAGCAAATCAACAAGGTATCTGGCACTGGAGAAAAATACTGCTAGTCTTGACTGGATGGATGCCTCTGC ACTTGTAGGTGAGGAGCCTACAATGTTAAGCTACTGCTGCCTCACATTTGAGCAAACCTCATGTATGATT CAGCAAGACCTCAGCCTTGCTTTTTGGCTAGTCTTGCCGGCAATAAAGCTGTCTTCTATCTAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5067523 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5067523 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10830 same embryo
	EMAGE:10831 same embryo
	EMAGE:10833 same embryo
	EMAGE:10828 same embryo
	EMAGE:10829 same embryo
	EurExpress:euxassay_003065 same experiment
	EMAGE:31371 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source