EMAGE mouse gene expression spatial database

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EMAGE:10854

Psmd12 proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 ( MGI:1914247)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003452. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003452_01	euxassay_003452_02	euxassay_003452_03	euxassay_003452_04

euxassay_003452_05	euxassay_003452_06	euxassay_003452_07	euxassay_003452_08	euxassay_003452_09

euxassay_003452_10	euxassay_003452_11	euxassay_003452_12	euxassay_003452_13	euxassay_003452_14

euxassay_003452_15	euxassay_003452_16	euxassay_003452_17	euxassay_003452_18	euxassay_003452_19

euxassay_003452_20	euxassay_003452_21	euxassay_003452_22	euxassay_003452_23	euxassay_003452_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10854_wholemount_strong.wlz

10854_wholemount_moderate.wlz

10854_wholemount_weak.wlz

10854_wholemount_possible.wlz

10854_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10854_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 20 21 22 23 24

limb

moderate

regional

moderate expression: see section 01 02 03 04 21 22 23 24

hindlimb

moderate

regional

moderate expression: see section 05 06 19 20

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

adrenal gland

moderate

regional

moderate expression: see section 09 10 11 18 19 20

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 16

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 16 17 18 19 20

pancreas

strong

regional

strong expression: see section 08 09 10 11 12 16 moderate expression: see section 18

central nervous system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

adenohypophysis

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

physiological umbilical hernia epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 24

vibrissa

strong

regional

strong expression: see section 05 06 07 08 19 20 21 22 24 moderate expression: see section 04 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 09

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23 24

vagus x ganglion

moderate

regional

moderate expression: see section 10 19

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 13

cervical ganglion

moderate

spotted

moderate expression: see section 11 17 18

thoracic ganglion

moderate

spotted

moderate expression: see section 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 12 13 17 18 19

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 05 07 08 09 10 18 19 20 21 22 23

cornea epithelium

weak

regional

weak expression: see section 02 03 04 24

cornea stroma

moderate

regional

moderate expression: see section 01 weak expression: see section 02 03 04 23 24

naris

moderate

regional

moderate expression: see section 11 12 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 12 15 16 17 18 19 20

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 11 12 13 15 16 17

heart

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

tongue

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08

midgut

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 12 14 15 16 17

lower jaw molar

moderate

regional

moderate expression: see section 06 07 08 09 18 19 20

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16 17

upper jaw molar

moderate

regional

moderate expression: see section 06 07 08 09 18 19 20

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

bladder

moderate

regional

moderate expression: see section 13 14 15

metanephros

moderate

regional

moderate expression: see section 08 09 10 11 18 19 20 21 22

genital tubercle of male

moderate

regional

moderate expression: see section 11 12 13 14 15 16

larynx

moderate

spotted

moderate expression: see section 12 13 14 15

lung

moderate

spotted

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 22 23 24

not examined

spotted

not examined expression: see section 10 11 12 13 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6224
Entity Detected:	Psmd12, proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 ( MGI:1914247)
Sequence:	sense strand is shown >T6224 ACTTCCGGTGCGAGTGACGAGTGGTGGCCGAACCTGGGGTACAGCAGGGGACTCTTAGGCAGGGACCATG GCGGACGGTGGCTCGGAGCGGGCCGATGGGCGCATTGTGAAGATGGAAGTGGACTACAGCGCCACGGTGG ACCAGCGCCTGCCTGAGTGCGAGAAGCTGGCCAAGGAAGGGCGGCTTCAAGAAGTCATCGAAACCCTTCT CTCTCTAGAAAAGCAGACCCGGACTGCCTCTGACATGGTGTCCACGTCCCGCATCTTGGTTGCAGTAGTG AAAATGTGCTATGAGGCTAAAGAATGGGACTTACTTAATGAAAATATTATGCTTCTGTCAAAACGACGGA GTCAGTTAAAACAAGCAGTTGCAAAAATGGTTCAGCAGTGCTGTACTTACGTGGAGGAGATCACAGACCT GCCAGTCAAGCTGCGATTGATTGATACTCTGCGGATGGTTACAGAAGGAAAGATTTATGTTGAAATTGAG CGTGCTCGGCTGACTAAAACCTTAGCAACTATTAAAGAGCAAAATGGCGATGTGAAAGAGGCTGCCTCCA TCCTTCAAGAGTTACAGGTGGAAACCTATGGGTCCATGGAGAAGAAGGAGCGAGTGGAATTCATTCTGGA GCAGATGAGACTCTGCCTAGCTGTGAAGGATTACATTCGCACACAGATCATTAGCAAAAAAATTAATACC AAATTCTTCCAAGAAGAAAACACAGAGAACTTGAAGTTGAAGTACTATAACTTAATGATTCAGCTGGATC AGCACGAGGGGTCCTATCTGTCCATCTGCAAACACTACAGAGCCATCTATGACACTCCCTGCATTCAGGC AGAAAGTGACAAGTGGCAGCAGGCTCTGAAAAGTGTTGTTCTCTATGTTATCCTGGCTCCTTTTGACAAT GAGCAATCAGATTTGGTTCACCGGATAAGCAGTGACAAGAAGCTGGAAGAAATTCCCAAATACAAGGATC TGCTGAAGCTTTTTACCACCATGGAGCTGATGCGCTGGTCCACTCTTGTTGAGGACTATGGGGTGGAGCT GCGAAAAGGCTCCTCAGAGACCCCGGCGACTGACGTTTTCAGCTCTACAGAAGAAGGGGAAAAGAGGTGG AAAGACTTGAAGAGCAGAGTGGTGGAGCACAACATCAGAATTATGGCCAAGTACTACACTCGGATAACAA TGAAAAGGATGGCACAACTTCTGGATCTCTCTGTGGATGAGTCAGAGGCTTTCCTCTCGAATCTAGTCGT TAACAAGACCATCTTTGCTAAAGTAGACAGGTTGGCTGGAGTCATCAACTTCCAGAGACCCAAGGATCCA AATAATTTATTAAATGACTGGTCTCAGAAACTGAACTCGCTGATGTCTCTGGTTAACAAAACGACACACC TCATAGCCAAAGAGGAGATGATACATAATCTACAATGAGGCTCTGGATGCTTTTAGAAGACACAAAATTG GAGGTCATTAAAAAAGAAAGACTGTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498952 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498952 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10858 same embryo
	EMAGE:10856 same embryo
	EMAGE:10855 same embryo
	EMAGE:10857 same embryo
	EMAGE:10853 same embryo
	EurExpress:euxassay_003452 same experiment
	MGI:4827487 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source