EMAGE mouse gene expression spatial database

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EMAGE:10859

Samd11 sterile alpha motif domain containing 11 ( MGI:2446220)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003349. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003349_01	euxassay_003349_02	euxassay_003349_03	euxassay_003349_04

euxassay_003349_05	euxassay_003349_06	euxassay_003349_07	euxassay_003349_08	euxassay_003349_09

euxassay_003349_10	euxassay_003349_11	euxassay_003349_12	euxassay_003349_13	euxassay_003349_14

euxassay_003349_15	euxassay_003349_16	euxassay_003349_17	euxassay_003349_18	euxassay_003349_19

euxassay_003349_20	euxassay_003349_21	euxassay_003349_22	euxassay_003349_23	euxassay_003349_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10859_wholemount_strong.wlz

10859_wholemount_moderate.wlz

10859_wholemount_weak.wlz

10859_wholemount_possible.wlz

10859_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10859_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5368
Entity Detected:	Samd11, sterile alpha motif domain containing 11 ( MGI:2446220)
Sequence:	sense strand is shown >T5368 CAGGGCTGCTGTCACAGGTGGGCCACAGGCTGCTGTCACAGGGCGCGTGCTGAGTGCCGGGCACCGCTGT CCCTGCCCTGGCTCTGGCTTCCTGTTTTTCCCTTCGCTACTGATGTGGGTGTTCTTACAGACCGTCAGGT GCACGTCGAAGCCGCTGCCTCGTTATGCCTGAGCATCCAAGACACTGTGACTTCCAGAGAGGCAACGTGG AGATCGGCCTTGGACCTGGAGGCGACCTGTTGGGCAAGAGGCTGAGCTGCCCCTGCATCACCAGTCACTG CTCTTCAGAGAAGAAGGCCAGAAGCAAGGACCCCCAAGGTGAAAGCCCGACTCTGGAAAAGGATGCAGCG ACTTCGCTGCTTCCCGAGCTAGAGTCTACAATGGCCCCCGAGGACCACTACCATCAGCTTATGTCGGCCC TGAGTGAGGCCAGCTCCTTTGAGGAAACTCAACGTCTCTACCACTTGGGTATCCCCAGCCACGATCTGCT TAGGGTACGCCAGGAAGTAGCAATTGCAACACTGAGGGGTCCCAGTGGCTTGGAAGTTCACCTGCCCTCG TCTACAGCAGACCATCGACGGAAGCAAGGCCTAGTTCAGCGCCGGGAAGGGGCGGTTCCAGCTGCTGCCA CATCTTTCTCTGAAAGGGAAATGTCACAGCCACCCCCTTTGCTGTCACCCCAGAATGCCGCCCACATCAC AATGAGCTCCCATCTCCGGCCTCCTTTTCTGGGGATGCCCACAGCTGTGTGTCAGACCCCAGGTTTCAGC TTCCTACCCTCCGCCCAGGCTGAGATGTTAGCTCGGCAGCAGGAACTTCTACGGAAGCAGAGCCTGGCTC GGCTGGAAATGTCAGAGTTGCTTAGGCAGAAAGAACTGGGCAGTGTTCACCGGCCACTGCTACCGGCACC AGAGGTGGCCTTGCATATCCCCGAGGGTCCCGACGAACTGCAGCGACGTGGCTCCATGCTGGTTTTGAAA CACAGCTCAGCACCACTACTGGCCCTGCCACCCCAGGGACCCCCAGGCCCAGGACCCCCTATCCCACCCA AGGAGTCTGCTCGAAGCCGGTCCGAAAAGGGGAGCCTTGGTGTCCAGCCCAGCCAACCCAAGGAGACGAC AGGGGCTGGCCTCTGGGCTCAAGAAGTCTCTGAGGAGCCATCCAAGGACTCAGATGGAGAGGACCCCGAG ACAGCAGCTGCCAGGGAAGGGACCTCCACTCCCAGCCAAGTCCCAGCAGGAGGGACCAGAGCAGAAGGAA GGGGGCTTCTATCAGGGTCCACACTGCCCCCTCCACTGCCCCTGGGATTCCCTTGCGGGGCAGTGAGCCC CTACTTCCACACAGGTGGGCAGCCTTAGCCACCCACTGATAGGGGATCCCGTGGGGAGAAAAATCATTTG TCATTAAGCCAAAGACTTTACCAATTCAGAGAAAGGTCAGCCAGTGGGGAAGGAGGCAAAGAAATGGGAA AGCCCAAGCCCTAGTGACATCCTTTCTATCTCCAGGTACCATGGGGGGGCTCTTCACTGATGAGGAGACC ACAACCCTTGAGGATGTCAACAAGTGGACTGTAGATGATGTCTGCAACTTCGTGGGGGGCCTTTCTGGCT GTGGCGAGTATTCGGAGAACAAGGAATAGACGGAGAGACTTTGCCTCTACTGACAGAAGAGCACCTTCTG AACACCATGGGACTGAAGCTAGGGCCTGCCCTCAAGATCAGGGCACAAGTGGCCAAGCGCCTGGGCCGTG TCTTCTACATGGCCAGCTTCCCTGTGGCCCTGCCGCTGCAGCCACCAAGCCTGCAAGCACCTGAGCTCAG CCCAGGACATCAACCTCTGTCGCCAGCAACCACCACCTCCCCGTATGAAGGGACCCACCTGCCCACAGGA CAAGCCTCACCCAAGCAGGAGAATGGGTCAGGGACCATAGCTCTGCTTTCAGGTGCCCCAGACCCTTCCC AGCTTCTCCAATGATCCGGCAGGCAACCGTCATACTCCGTGATTCCCAGTCAAATTGCTAACCTCACTCC GCCCCCAGGGTATTTCCCCTTTTTAGATGCAAAAATGCCGAGAGTATTTTTAAAAGGAAAACATAAGGCT TAAGAAGAAAAGCAATGAGCTGGGCAGAGGTGGCGCACGCCTTTAATCCCAGCACTTGGGAGGCAGAGGC AGGCGGATTTCAGAGTTCGCGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGATAGCCAGGGCTACACAGA GACACCCTGTCTCGAAAAAACAAACAAACAAACAAACAAACAAAAGTAATGGGTTTATCTTCTAGAGGTA ACTGCAGTGCTCTGCAGACAAACCAAAGACAAAGTGGAGAGGAGAGGCCATGGAGCCGCCCTCCCCACTC TGAACTGGGACAACGTCAGGGTCAGCCTTCGGCTTCCGGCAGCAGAAGGCTCTGCAGAAACAGTTCCCTC CCTTTCAGGGTCAAAATGGCTCCTAGTACACTTGTTCTGCTGTGCTGTAACAATAAAAGCCTGTTTACGC TCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5363470 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5363470 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10863 same embryo
	EMAGE:10861 same embryo
	EMAGE:10862 same embryo
	EMAGE:10860 same embryo
	EurExpress:euxassay_003349 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source