EMAGE mouse gene expression spatial database

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EMAGE:10861

Eif4a3 eukaryotic translation initiation factor 4A3 ( MGI:1923731)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003305. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003305_01	euxassay_003305_02	euxassay_003305_03	euxassay_003305_04

euxassay_003305_05	euxassay_003305_06	euxassay_003305_07	euxassay_003305_08	euxassay_003305_09

euxassay_003305_10	euxassay_003305_11	euxassay_003305_12	euxassay_003305_13	euxassay_003305_14

euxassay_003305_15	euxassay_003305_16	euxassay_003305_17	euxassay_003305_18	euxassay_003305_19

euxassay_003305_20	euxassay_003305_21	euxassay_003305_22	euxassay_003305_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10861_wholemount_strong.wlz

10861_wholemount_moderate.wlz

10861_wholemount_weak.wlz

10861_wholemount_possible.wlz

10861_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10861_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 09 17 18 19

vibrissa

strong

regional

strong expression: see section 06 07 09 22 23 moderate expression: see section 08

testis

strong

regional

strong expression: see section 05 06 07 08 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6223
Entity Detected:	Eif4a3, eukaryotic translation initiation factor 4A3 ( MGI:1923731)
Sequence:	sense strand is shown >T6223 CCGGTTCCCGCCGCGGCTTGCAGTTGTCTTTCTGCGGAATCCGGATCATGGCGGCTAACGCCACGATGGC GACGTCTGGCTCGGCGCGGAAGCGGCTGCTCAAAGAGGAGGACATGACCAAAGTGGAGTTCGAGACGAGC GAGGAGGTGGACGTGACCCCCACGTTCGACACCATGGGCCTGCGAGAGGACCTGCTGCGCGGCATCTACG CCTACGGTTTTGAAAAACCTTCAGCGATTCAGCAGCGTGCTATCAAGCAGATAATTAAAGGGAGAGATGT CATTGCACAGTCTCAGTCTGGCACAGGCAAGACGGCCACCTTCAGTGTCTCAGTGCTGCAGTGCTTGGAT ATCCAGGTTCGAGAAACCCAAGCTTTGATCCTGGCTCCAACCAGGGAGTTAGCGGTGCAGATTCAGAAGG GTCTGCTCGCGCTGGGGGATTACATGAACGTGCAGTGCCATGCCTGCATTGGGGGCACCAACGTCGGCGA GGACATCCGGAAGCTGGACTACGGACAGCACGTGGTGGCAGGCACGCCGGGACGCGTCTTTGATATGATC CGCCGGAGAAGTTTACGGACACGGGCTATCAAGATGCTGGTTTTGGATGAGGCTGATGAAATGTTGAACA AAGGTTTCAAGGAGCAGATCTATGATGTGTACAGGTACTTGCCACCAGCCACACAGGTCGTCCTCATCAG CGCCACACTGCCTCATGAGATCCTGGAGATGACCAACAAGTTCATGACCGACCCCATCCGCATCTTGGTG AAGCGTGATGAGTTGACTCTGGAAGGCATCAAACAGTTCTTTGTGGCTGTGGAAAGAGAGGAATGGAAAT TTGATACTCTATGTGATCTCTATGACACGCTGACCATCACCCAGGCCGTCATCTTCTGCAACACCAAGCG GAAGGTTGACTGGCTGACAGAGAAAATGAGAGAAGCCAATTTCACTGTGTCGTCCATGCATGGAGACATG CCCCAGAAAGAACGAGAGTCTATCATGAAGGAGTTCCGGTCAGGTGCCAGCCGGGTGCTCATCTCCACAG ACGTCTGGGCCCGGGGCCTGGATGTCCCTCAGGTGTCCCTCATCATTAACTACGACCTGCCCAACAACAG AGAACTGTACATTCACAGAATTGGGAGATCGGGTCGGTATGGACGAAAAGGTGTGGCCATCAATTTTGTG AAGAATGATGACATCCGGATTCTCAGGGACATTGAGCAGTACTACTCCACCCAGATAGACGAGATGCCCA TGAATGTGGCTGACCTCATCTGAAGCTGGTGCTGGTGCACCGAGGCCCTGTTTGGAAACGGAAGCTTCTA TTTAATGGGGTTGATATGGACTGTCTTCTCGGAGCCCCTCGGGAAGACCCTCAGCCTCCGCCATCTTTTC TAATGCACATGTACATAATCCGATGCTTAACCTTTTACATTAAACGTGGACATTTTCCTGTGAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498954 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498954 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10863 same embryo
	EMAGE:10862 same embryo
	EMAGE:10860 same embryo
	EMAGE:10859 same embryo
	EurExpress:euxassay_003305 same experiment
	MGI:4824512 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source