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EMAGE:10871

Erdr1 erythroid differentiation regulator 1 ( MGI:2384747)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10871 EMAGE:10871 EMAGE:10871 EMAGE:10871 EMAGE:10871
"Pseudo-wholemount" of euxassay_003307. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003307_01 euxassay_003307_02 euxassay_003307_03 euxassay_003307_04
EMAGE:10871 EMAGE:10871 EMAGE:10871 EMAGE:10871 EMAGE:10871
euxassay_003307_05 euxassay_003307_06 euxassay_003307_07 euxassay_003307_08 euxassay_003307_09
EMAGE:10871 EMAGE:10871 EMAGE:10871 EMAGE:10871 EMAGE:10871
euxassay_003307_10 euxassay_003307_11 euxassay_003307_12 euxassay_003307_13 euxassay_003307_14
EMAGE:10871 EMAGE:10871 EMAGE:10871 EMAGE:10871 EMAGE:10871
euxassay_003307_15 euxassay_003307_16 euxassay_003307_17 euxassay_003307_18 euxassay_003307_19
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euxassay_003307_20 euxassay_003307_21 euxassay_003307_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10871Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10871_wholemount_strong.wlz
10871_wholemount_moderate.wlz
10871_wholemount_weak.wlz
10871_wholemount_possible.wlz
10871_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10871_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6235
Entity Detected:Erdr1, erythroid differentiation regulator 1 ( MGI:2384747)
Sequence:sense strand is shown

>T6235
GTCCGTTCTCTTTTAGCCGCAGCTATGGTTTCTGCCCTAATTATTCTTGTCCTTATTTGTAATTTAATTC
TTAATTTAATTTAATTTATAATTTTGTTGTAAGTTTCTCTGTGGGCGTGAATGGAAAGTCTAACCCGTGT
TTCTCTGTTCAGCGTCCGCCGGTCACGGCCGCCGCCCCCAGCGACGTCACCCACACGCGCAGAAGCGGAC
GCCGCGGTCAAGATGTCTCTGCCATGCCCACGGGACGCACGGACGGACGGACGCACGGACGGACTCCACA
AGGTAGGAAGCCTGCGCCGACCGCACCGCTGCACCCACCACAGCACACAGGACACACGCGGGCCCCGCGC
CCGCCCAGGCACACGCGGCACACACGGCACACACGGCAGGCAGGCCAGGCACACGCATCCGCAGGACCCG
CCGCACCCGCCACGCAGACACGGACGAGCCGCCGCGGTCAAGATGTTCACCCGCCGCGGTCAAGATGTAT
GTGCCACCGACCCTCGCCCCGCTGGACGGACGGACGGACGCACGCACGCCGTCAGCGTCCACCGGTCACT
GCCGCCGCCCACAGTGATGTCACCCACGAAAGCACACACGTAGAAGCGGACGCCGTGGTCAAGATGTCTC
TGCCATCCCCACAGGACGGACGGACGGACTCCACAAGGTGCGCGTGTCGCCGAGGCCGCCAGGACGGAGC
GATTCTCACGGAGGAAGGAGCACGCCAACAGGGCCTGACTGCGTACAGAAATGCCCCCCCTCAATAAAAT
TGCAGTTGAAATGGAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3499536 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3499536 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10869 same embryo
 EMAGE:10873 same embryo
 EMAGE:10874 same embryo
 EMAGE:10870 same embryo
 EMAGE:10872 same embryo
 EurExpress:euxassay_003307 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS