EMAGE mouse gene expression spatial database

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EMAGE:10872

2610027L16Rik RIKEN cDNA 2610027L16 gene ( MGI:1915092)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003329. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003329_01	euxassay_003329_02	euxassay_003329_03	euxassay_003329_04

euxassay_003329_05	euxassay_003329_06	euxassay_003329_07	euxassay_003329_08	euxassay_003329_09

euxassay_003329_10	euxassay_003329_11	euxassay_003329_12	euxassay_003329_13	euxassay_003329_14

euxassay_003329_15	euxassay_003329_16	euxassay_003329_17	euxassay_003329_18	euxassay_003329_19

euxassay_003329_20	euxassay_003329_21	euxassay_003329_22	euxassay_003329_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10872_wholemount_strong.wlz

10872_wholemount_moderate.wlz

10872_wholemount_weak.wlz

10872_wholemount_possible.wlz

10872_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10872_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6241
Entity Detected:	2610027L16Rik, RIKEN cDNA 2610027L16 gene ( MGI:1915092)
Sequence:	sense strand is shown >T6241 GCGCGCCGGCGGTTGTGGGAGCTGCAACCGGGAAGCGTGAACGGCTGGTCCCAAAGCACGCATGGGGTTG GGGCCGCGTTCCGCTCACAAAGCGCGACGCCAGTTCTCGGGTGCCGGCCGGCGGGGACGCGCGGCCCGGG GTTCGGGACGTCCCCCTCCCGGCCGCGATGGTTATCCCCGGCTGCCGGCAGCCGCGCGTGCGGAGCAGGC CCCCGAGGCTCTCCCGCATCTGAGCCCGGAGGCTCTTGGATATTTCCGACGGGCGCTGTCAGCGCTAAAA GTGGCTCCCGACGCCGCCGAAGAACGAGAGTTGATGGCGCGCAATATTTTGAAGGAAGTGGAGGCTCAGG CTCTAGCCTTGGCCACGAACAGGACTGGTAGCGAAATGCTGCAGGAGCTTTTGGGGTTCAGTCCTTTGAA ACCCCTGTGTCGAGTGTGGGCCGCTCTGCGCCCCAACTTGCGCTTCGTGGCTTGTCATCGGTGTGGGGTC CACGTATTACAAAGTGCTTTGCTGCAGCTACCTCGATTGCTGAGGCGTCCTGCGGAGGCAGAGGAGGAGG AGGAGGAAGAGGAGGAGGGTGGTCCCTCGCAAACTCTGGAGGAGCTAGTCCTGGGACTAGCCGCTGAAGT GTGTGATGATTTTCTGTTCTTCTGTGGAGACACGCATGGCAGCTTCGTGGTCAGAACTCTGCTGCAGGTC TTGGGAGGTACTCTTTTGGAGTCCGAGAGAGGCAAGCCCCGTGGCTCCCAGTCGTCTGAAACACAGAGGA CTTCAGCTCGGGAATGTAAACCAACTGATTTTGAGGTTCCTAAAACCTTCTTGAATCGCCTTCAGGACCT GAGCGCCTGCTTTCTGAAGGACATTGCTGTATTTATCACCGACAAGATCTCCAGCTTTTGTCTTCAGGTG GCCTTACAAGTCCTCCACCAAAAATTACCTCAGCACTGTGCCCACCTCTGCGATGCAGTCATTGATTACC TGAGTTCTCGGAATTCTTCAGCAGATGGCAGCCCTCTACTCCTGTTTCTTCGGGATCAGACGAGCTCTAG ACTCTTGGAGCAGGTGCTGCTGGTATTAGAAGCAGAGAGACTCCAGCGCCTCTATAAGGACCACTTCCAG GGTCAGCTATGCTCCTTGGCTGAGCATCCCATTGCGAACTTCCCTTTGCAGCGCTTGCTGGATGCAATCA CTTCCCCTGAGCTGCTGTCCCTTGTGTTTGAGGAGCTGAGCCCTGCCTTGGAAATGGTGCTGGCCCAGGG CCACCCAGGGGTCGTCGTCGCCCTGGTGGAAGCCTGCCGCAGGGTTGGGACTCACCAAGCCCAGGTCTTG CAGCTGTTATTTGAGGCATTTCATTGTGCAGAGCCCCCGTCCCGGCAAGCAGCCTGCGTGCCTCTCTTTG CTGCTTTGTTGGCTTATGAGGTATACTATGAACTAATGGAGGAGGAGGGGGCTGTGCCTGCAGAGCATCA GGTTGAAATGGCCACAGCCAGAGCGCTGAGGGAGGTGACTGTACTTGGGTCTCTACTGCTCCAGCATTTG CTGTATTTTTCTAATCCTGGACTTGTAGTTCGAAGTCTGAGTGCCTTGACCGGACCACAGCTTCTGACTC TGGCCCAAAGCCCCGCTGGCTCTCATGTATTTGATGCCATCCTGAGTAGCCCCTCAGTGACACACAAGCA GCGTCGTCGTGTGCTGAAGACCCTAAAGGGACAGTATGTGGCTCTTGCCTGTAGCCGCCATGGCAGCCGT GTATTAGATGCCATCTGGAGTGGAGCAGCTTTGGGGGCTCGGAAGGAAATTGCTGCTGAATTGGGAGAAA AGAACCAGGAGTTGATTCAAGATCCTTTTGGCCACCATGTGGCTCGAAATGTGGCCCTTACTACCTTCTT GAAGAGGCGAGAGGCTTGGGAACAACAGCAAAGTACTGTGGCCAAGCGGAGGCGGGCATTGAGCTCTATA CTTGAAGACTGAGACTTGCAACGGGACTCGGTCTTGATGGGGAGGGGAACATGCTTACCCTTCCTGTTTA CATTGGATCCAAAAGTCTTACTGCAGCCAGATGTAGTAACATGCACAAAACTGTAAACCTACTATTCCAG AGGGTAAAACAAGAATATCAAGAATTCAAGGCTAGCCTGCTCTGTGGCAGCTAGATCTTGTTAATCTCTA TGTCCATCTGCTATTCTCTCCCCTCTCCCAAGACATACTGGTAAAGACTTTTGTATTTTTCTTGGAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3499986 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3499986 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10871 same embryo
	EMAGE:10869 same embryo
	EMAGE:10873 same embryo
	EMAGE:10874 same embryo
	EMAGE:10870 same embryo
	EurExpress:euxassay_003329 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source