EMAGE mouse gene expression spatial database

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EMAGE:10922

Dctd dCMP deaminase ( MGI:2444529)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003473. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003473_01	euxassay_003473_02	euxassay_003473_03	euxassay_003473_04

euxassay_003473_05	euxassay_003473_06	euxassay_003473_07	euxassay_003473_08	euxassay_003473_09

euxassay_003473_10	euxassay_003473_11	euxassay_003473_12	euxassay_003473_13	euxassay_003473_14

euxassay_003473_15	euxassay_003473_16	euxassay_003473_17	euxassay_003473_18	euxassay_003473_19

euxassay_003473_20	euxassay_003473_21	euxassay_003473_22	euxassay_003473_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10922_wholemount_strong.wlz

10922_wholemount_moderate.wlz

10922_wholemount_weak.wlz

10922_wholemount_possible.wlz

10922_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10922_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 11 16 17 18 20

pancreas

moderate

regional

moderate expression: see section 07 08 09 10 11

vibrissa

moderate

regional

moderate expression: see section 05 06 07 08 09 21 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 17 18 19 20

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 14 15 16 17 18

midgut

strong

regional

strong expression: see section 09 10 11 12 13 moderate expression: see section 14 15 16 17

metanephros

moderate

regional

moderate expression: see section 06 07 08 09 10 16 17 18 19 20

lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 07 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6753
Entity Detected:	Dctd, dCMP deaminase ( MGI:2444529)
Sequence:	sense strand is shown >T6753 CGGACGCGTGGGCGCGCACGGCGGCTGTGTGGGGTGGACGGTGCGAGCGTGGTCTGCACCTCGAAGGAGC GGACGTGACGTATGACTTCTACACCCAACATGAGTGACATTTCCTGCAAAAAACGGGATGACTATCTGGA ATGGCCCGAGTATTTCATGGCAGTGGCCTTCTTGTCGGCACAAAGAAGCAAGGACCCAAGTTCCCAGGTG GGGGCCTGCATTGTGAACACAGAAAACAAGATTGTTGGGATCGGGTACAATGGCATGCCAAATGGGTGCA GTGATGACCTGTTGCCTTGGAGGAGAACAGCAGAAAATAAGCTGGATACTAAATATCCTTATGTGTGCCA TGCCGAGCTGAACGCCATCATGAACAAGAACTCGGCTGATGTGAAGGGCTGCAGCATGTACGTCGCCTTG TTCCCGTGTAACGAATGTGCTAAACTCATCATCCAGGCAGGTATAAAAGAGGTTATCTTCATGTCGGATA AATACCACGACAGCGAGGAGACGACGGCTGCTAGGCTCCTGTTTAAGTTGGCTGGGGTTACGTTCAGAAA ATTCACACCGAAGTACAGCAAGATTGTCATTGACTTTGATTCGATCAATAGCCGACCGAGTCAGAAGCCT CAGTGAGTTTCGTCTCGTTAAAGCCCCAGAAGAAGATGACACCCCACCACGTGCGTGTGCACACACACAC ACACACACACACATACACACAAGGGGCTCCCCAAACCTTTCATCTTGAAGTCCCACGGAACTTTTTAATA GCACAACACTAAACATCTAAAGAATATAAAACTTGGAATCTTCTCACCCGTCACGTGGAGCTCCATCTGT TTCAGCTGTTGTTGCAGTTTGGTTTGGAAACTAAGGGACCTGCCCACAGGAGCTGGAGTGAAGGTCTCTC CCCTTCCAGCCCGTCTGCCAGAGAGTGTCAGCTCTGTCATCCTCCATATTTCGACAGCCGCTCCTTGCTG CTACACGGGGTAGGAGGACATACAAATAATTGATTCTCGTTCTTAGCAGGGATGAACAGGCACACTGAGG TGAACATCTGGCCCAAATGAAGCATACCCTGTCACGCCCTTGGGAAAACAAATAGGACCGAACAAAGCTA CATGAACACACCTGCTGTAGAGTTGAGGGGACCTCTCACCTGGTCACATGAGGTTGAGGAGGGCCTAGTG TCCTGGCAGATGATCAGTACAGTGTCCCCCTCAAGTCACACTTTCCTGCCTTACACAGAGCAGTAGCATA CCATCCCTCTGGAGGGTAGGGGACCCCTGCGGTTCCAGCCTCTTAGCCTTGGTTTGTTGGTCACATAGCA CTGTTAAATTTATCCGAGGCATAAGTAGTTCCTAGAGGAGACAGCCTATCACTCCCCCTAGACCAGGCGC TTTGCGGTGACAGCTGGAGCTATATGGATGGGTGATGAGAGACGTTCCCCCATCCCGTGTCTCCTGCTGT GTGGACACATGTCACTCCTGCTCCACATGCCAACATGGTTCATTTCCAAGGGCACCCTATAACCAGAGAG CACATTAATTCCTGTCCTATAAGAGGGCTGTGGGAAACTGTGTACCTGGAGGTGGTCCATGCCAGATCCT CATTTGTGCCTTCAGCTTCATAGGCAGCATACGCCTGCTGCCCCATTGTGGGTGGGGGTGCCCCACTGCG TACTCTGTTTTTAATTTCATTTAACTTCTAGCAGACATGCTCAGTGTCTTTGATAAAATAGTGTATTTTT TCATGTGTTTTTATTTCATTTCTAAGATTTTTTTTTAACACTCCGTATTCTGGAGCATTTTTTTACAATT TGGATCTCTGCCAGTATTGTTAAAATTTTATTAAAATCATCCAGCACCAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3672932 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3672932 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10920 same embryo
	EMAGE:10924 same embryo
	EMAGE:10919 same embryo
	EMAGE:10923 same embryo
	EMAGE:10921 same embryo
	EurExpress:euxassay_003473 same experiment
	MGI:4824255 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source