EMAGE mouse gene expression spatial database

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EMAGE:10923

Scyl1 SCY1-like 1 (S. cerevisiae) ( MGI:1931787)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003377. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003377_01	euxassay_003377_02	euxassay_003377_03	euxassay_003377_04

euxassay_003377_05	euxassay_003377_06	euxassay_003377_07	euxassay_003377_08	euxassay_003377_09

euxassay_003377_10	euxassay_003377_11	euxassay_003377_12	euxassay_003377_13	euxassay_003377_14

euxassay_003377_15	euxassay_003377_16	euxassay_003377_17	euxassay_003377_18	euxassay_003377_19

euxassay_003377_20	euxassay_003377_21	euxassay_003377_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10923_wholemount_strong.wlz

10923_wholemount_moderate.wlz

10923_wholemount_weak.wlz

10923_wholemount_possible.wlz

10923_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10923_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 18 19 20 21 22

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 15 16

pons mantle layer

weak

regional

weak expression: see section 08 17

otic capsule

weak

regional

weak expression: see section 01 02 03 04 08 19 21 22

hyoid

moderate

regional

moderate expression: see section 10 11 17 18

meckel's cartilage

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 19 20 21 22

lower jaw mesenchyme

moderate

regional

moderate expression: see section 07 08 09 10 11 19 20

palatal shelf

moderate

regional

moderate expression: see section 07 08 09 10 11 18 19 20 21

orbito-sphenoid

moderate

regional

moderate expression: see section 03 04 05 06 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6751
Entity Detected:	Scyl1, SCY1-like 1 (S. cerevisiae) ( MGI:1931787)
Sequence:	sense strand is shown >T6751 CCCACGCGTCCGGGAGGACCCGGAGTGGAGGCTCCCGAGCCCGTGGCGGCGGCGACGATGTGGTTCTTTG CCCGGGACCCGGTCCGGGACTTCCCGTTCGAGCTGAGCCCTGAGCCCCCCGAAGGCGGGCCGCCCGGGCC CTGGATCCTGCACCGAGGCCGCAAAAAGGCCACAGGCAGCGCAGTGTCCATCTTCGTGTATGATGTGAAA CCGGGAGCTGAAGAGCAGACCCAGGTGGCCAAAGCTGCCTTCAAACGCCTCAAAACTCTCCGACACCCCA ACATCCTGGCCTATATCGATGGGTTGGAGACAGAAAAGTGCCTCCACATCGTGACAGAGGCTGTGACCCC CCTGGGAACATACCTCAAGGCACGAGCAGAAGCAGGTGGCCTGAAGGAGCAGGAGCTGTCATGGGGGTTA CACCAGATCGTGAAAGCCCTCAGCTTCCTGGTCAACGACTGCAACCTCATCCACAATAATGTCTGCATGG CCGCTGTGTTTGTGGACAGGGCTGGCGAGTGGAAACTTGGGGGTCTGGACTACATGTACTCGGCACAGGG CAACGGCGGGGGACCACCCAGCAAGGGGATCCCGGAGCTCGAGCAGTATGATCCCCCGGAGCTGGCTGAC AGCAGTAGCAGAGCAGTCAGAGAGAAGTGGTCAGCAGACATGTGGCGCTTGGGCTGCCTCATCTGGGAAG TTTTCAATGGGTCTCTACCTCGGGCAGCTGCCCTGCGCAACCCTGGGAAGATCCCCAAATCCCTGGTGAC CCATTACTGTGAACTGGTGGGAGCTAACCCAAAAGTACGTCCCAACCCGGCCCGCTTCCTGCAGAACTGC CGGGCACCCGGTGGCTTCATGAGCAACCGCTTTGTTGAGACCAACCTCTTCCTGGAGGAGATTCAGATCA AAGAGCCAGCTGAGAAGCAGAAGTTCTTCCAAGAGCTGAGCAAGAGTCTAGACTCATTTCCCGAAGATTT CTGTCGACACAAGGTGCTGCCCCAGCTACTGACTGCCTTTGAGTTTGGCAATGCTGGGGCCGTGGTCCTC ACACCTCTCTTCAAGGTGGGAAAATCCCTCCGTGCTGAAGAGTACCAGGAGAAGATCATCCCTGTGGTAG TTAAGATGTTCTCATCCACCGACCGGGCCATGCGCATCCGCCTCCTCCAGCAGATGGAGCAGTTCATCCA ATACCTTGATGAGCCAACAGTCAACACGCAGATTTTCCCCCACGTCACACATGGCTTCCTGGACACCAAC CCCGCCATCCGCGAGCAGACGGTCAAGTCCATGCTGCTCTTGGCCCCAAAGCTGAATGAGGCCAATCTCA ATGTGGAACTGATGAAGCACTTTGCAAGGCTACAAGCCAAGGACGACCAGGGTCCTATCCGCTGCAACAC CACGGTCTGCTTGGGCAAAATCGGCTCCTATCTCAGTGCTAGTACTAGACACAGGGTCCTCACCTCCGCC TTCAGCAGAGCCACTAAGGACCCATTTGCACCATCCCGGGTTGCGGGTGTCCTGGGCTTTGCTGCCACAC ACAATCTCTATTCGATGGACGACTGTGCCCATAAGATCCTGCCTGTGCTCTGTGGCCTTACTGTGGACCC TGAGAAATCTGTGCGGGACCAGGCCTTTAAGACCATTCGAAGCTTCCTGTCCAAATTAGAGTCTGTGTCA GAGGATCCCACCCAGCTGGCAGAAGTAGAGAAGGATGTCCATGCAGCGTCCAGTCCTGGAACAGGAGGAG CTGCAGCCAGCTGGGCAGGCTGGGCTGTGACTGGGGTATCCTCTCTCACCTCCAAGCTGATCCGAGCACA CCCCACGCCTGTGCCGTCTGATACCACTGTGCCCCAGAGACCAGTGCCAGAGGGAAATCCTGCTCCAGCC CCTGCCCTTGCCCAAGCTATCCCTGCAACCTCAGGGCACTGGGAGACACAGGAAGACAAGGACACTGCAG AAGACAGCGCCACTGCTGACAGATGGGACGATGAGGACTGGGGCAGCTTGGAGCAGGAAGCTGAATCCGT GTTGGCACAGCAGGATGACTGGAGTGCCAAGGGCCAAGGAAGCCGAGCTGGACAGGTCAACCACCCAGAC CACAAATCTCTGGAATCACATTGGAGCAGCTGGGAAGTTGAGGGCTCCTGGGACCAGGGCTGGCAGGAAC CCAGCTCTGTGGAGCCACCTCCAGAAGGCACTCGGCTAGCTAGCGAATATAACTGGGGTGGTGCAGAGCC CAGTGACAAGGGCGACCCCTTTGCTGCCCTGTCTGTTCGTCCCAGCGCTCAGCCCAGGCCAGACCCAGAC TCCTGGGGTGAAGACAACTGGGAAGGCTTGGAGGCTGAGAGCAGACAGGTAAAGGCAGAGCTGGCCCGGA AAAAGCGAGAGGAAAGGAGAAGAGAAATGGAAGCCAAACGGGCAGAGAAAAAGACCACCAAGGGGCCCAT GAAGCTGGGAGCCCGGAAGCTGGACTGACAACCCCACCCCCAAGCCACTGGGCTTCCAACCACTGGAGAG CAGGCCCGGCGGATGTATTTATTGTACAAACCATGTGAGCCTGGTCAGCAGGTCAGGCACATCTAGTGTA CATAATCAGAGCCACAATAAATTCTATTTCCCAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3672524 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3672524 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10920 same embryo
	EMAGE:10924 same embryo
	EMAGE:10919 same embryo
	EMAGE:10922 same embryo
	EMAGE:10921 same embryo
	EurExpress:euxassay_003377 same experiment
	MGI:4827917 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source