EMAGE mouse gene expression spatial database

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EMAGE:10930

Fabp3 fatty acid binding protein 3, muscle and heart ( MGI:95476)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003367. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003367_01	euxassay_003367_02	euxassay_003367_03	euxassay_003367_04

euxassay_003367_05	euxassay_003367_06	euxassay_003367_07	euxassay_003367_08	euxassay_003367_09

euxassay_003367_10	euxassay_003367_11	euxassay_003367_12	euxassay_003367_13	euxassay_003367_14

euxassay_003367_15	euxassay_003367_16	euxassay_003367_17	euxassay_003367_18	euxassay_003367_19

euxassay_003367_20	euxassay_003367_21	euxassay_003367_22	euxassay_003367_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10930_wholemount_strong.wlz

10930_wholemount_moderate.wlz

10930_wholemount_weak.wlz

10930_wholemount_possible.wlz

10930_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10930_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

4th ventricle lateral recess

strong

regional

strong expression: see section 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 09

ventral grey horn

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16

heart ventricle

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

meckel's cartilage

moderate

regional

moderate expression: see section 08 09 10 19 20 21 22 23 weak expression: see section 04 05 06 07

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 13 17 18

lower jaw molar

moderate

regional

moderate expression: see section 08 09 10 18 19 20 21 22

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 13 17 18

upper jaw molar

moderate

regional

moderate expression: see section 08 09 10 18 19 20 21 22

cranium

moderate

regional

moderate expression: see section 01

orbito-sphenoid

strong

regional

strong expression: see section 04 05 06 23 moderate expression: see section 01 02 03 07 22 weak expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5980
Entity Detected:	Fabp3, fatty acid binding protein 3, muscle and heart ( MGI:95476)
Sequence:	sense strand is shown >T5980 GTGCCTGCTCGCCTCCTCACTCATCGCACCATGGCGGACGCCTTTGTCGGTACCTGGAAGCTAGTGGACA GCAAGAATTTTGATGACTACATGAAGTCACTCGGTGTGGGCTTTGCCACCAGGCAGGTGGCTAGCATGAC CAAGCCTACTACCATCATCGAGAAGAACGGGGATACTATCACCATAAAGACACAAAGTACCTTCAAGAAC ACAGAGATCAACTTTCAGCTGGGAATAGAGTTCGACGAGGTGACAGCAGATGACCGGAAGGTCAAGTCAC TGGTGACGCTGGACGGAGGCAAACTCATCCATGTGCAGAAGTGGAACGGGCAGGAGACAACACTAACTAG GGAGCTAGTTGACGGGAAACTCATCCTGACTCTCACTCATGGCAGTGTGGTGAGCACTCGGACTTATGAG AAGGAGGCGTGACCTGGCTGCTCCGTCACTGACCGCCCGCTCCTCTGCCAACTGGCCACCCCTCAGCTCA GCACCATGCTGCCTCATGGTTTTCCCCTCTGACATTTTGTATAAACATTCTTGGGTTGGGATTTTTCTGG AGATACGGGGCATCAGCCTGGACCCAGTTCCTACTATGTATGTGGTTTATTTTTTAAAACTGTATCCAAA GGGTGCTCCAAGGTCAATAAAGCAGAACCAAGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:3485639 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3485639 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10927 same embryo
	EMAGE:10929 same embryo
	EMAGE:10926 same embryo
	EMAGE:10925 same embryo
	EMAGE:10928 same embryo
	EurExpress:euxassay_003367 same experiment
	MGI:4824675 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source