EMAGE mouse gene expression spatial database

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EMAGE:10935

Ctps cytidine 5'-triphosphate synthase ( MGI:1858304)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003471. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003471_01	euxassay_003471_02	euxassay_003471_03	euxassay_003471_04

euxassay_003471_05	euxassay_003471_06	euxassay_003471_07	euxassay_003471_08	euxassay_003471_09

euxassay_003471_10	euxassay_003471_11	euxassay_003471_12	euxassay_003471_13	euxassay_003471_14

euxassay_003471_15	euxassay_003471_16	euxassay_003471_17	euxassay_003471_18	euxassay_003471_19

euxassay_003471_20	euxassay_003471_21	euxassay_003471_22	euxassay_003471_23	euxassay_003471_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10935_wholemount_strong.wlz

10935_wholemount_moderate.wlz

10935_wholemount_weak.wlz

10935_wholemount_possible.wlz

10935_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10935_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6207
Entity Detected:	Ctps, cytidine 5'-triphosphate synthase ( MGI:1858304)
Sequence:	sense strand is shown >T6207 CGGCTGGCGCATGCGGCCCGGCCGAGCACTGGCTGCCCGAGGCTCACCACGCGCGCGCCGGCCTTGCTGT GGCTGACACGAGACTCCGAAGCCGGCCTCAGGTTAAAAGAAAAATGAAGTACATTCTAGTTACTGGTGGT GTTATATCAGGAATTGGAAAAGGAGTCATCGCCAGCAGTGTGGGCACAATACTTAAATCATGTGGCTTAC ATGTAACATCAATTAAGATTGACCCCTATATTAACATTGATGCAGGAACATTCTCTCCTTATGAACATGG AGAAGTCTTTGTGCTGGATGATGGTGGAGAAGTTGACCTTGACTTGGGAAATTATGAGCGATTCCTTGAT ATCCGTCTCACCAAGGACAATAATCTGACCACAGGGAAGATATACCAGTACGTCATTAACAAGGAGCGCA AAGGGGATTACTTAGGGAAGACTGTCCAGGTTGTCCCTCACATCACTGATGCAATCCAAGAGTGGGTGAT GAGACAGGCATTAATACCCGTAGACGAAGATGGCTTAGAACCTCAAGTGTGTGTTATTGAGCTTGGTGGC ACAGTGGGAGACATTGAAAGCATGCCCTTCATTGAGGCCTTCCGCCAGTTCCAGTTCAAGGTCAAGAGGG AAAACTTTTGTAATATCCATGTCAGTCTGGTTCCTCAGCCAAGTTCAACAGGGGAACAGAAGACAAAACC CACCCAGAACAGTGTTCGGGAACTTAGAGGACTCGGGCTTTCTCCAGACTTAGTGGTGTGCAGGTGCTCA AATCCTCTTGACACATCTGTGAAAGAGAAAATATCAATGTTTTGCCATGTGGAACCTGAACAAGTGATCT GTGTTCATGATGTTTCATCCATCTACCGGGTACCCTTGTTGTTAGAAGAGCAAGGGGTTGTAGACTACTT TCTTCGGAGACTTGACCTTCCTATTGAGAGACAGTCACGAAAGATGCTGATGAAATGGAAAGAGATGGCA GACAGGTATGACCGCTTGCTGGAGACCTGCTCGATCGCTCTTGTGGGCAAATACACCAAATTCTCAGACT CGTACGCCTCTGTCATTAAAGCGCTAGAGCACTCTGCATTGGCCATTAACCACAAGCTGGAGATCAAGTA CATCGATTCCACAGACCTGGAGCCAAGTACCCTGCAGGAAGAGCCTGTGCGCTACCATGAGGCATGGCAG AAGCTCTGCAGTGCTCATGGAGTGCTGGTTCCAGGAGGATTCGGTGTTCGGGGAACAGAAGGAAAAATTC AAGCAATTGCTTGGGCTCGGAAACAGAAGAAGCCTTTTTTGGGTGTGTGCTTAGGAATGCAGCTAGCAGT GGTAGAATTTTCAAGAAATGTGCTGGGATGGCAAGATGCCAATTCTACAGAGTTTGACCCCAAGACTAGT CACCCTGTGGTTATAGACATGCCAGAACATAACCCTGGGCAAATGGGTGGAACCATGAGGCTGGGCAAGA GGAGAACCCTGTTCCAGACCAAGAACTCAGTCATGAGGAAACTCTATGGAGACACAGACTACTTGGAAGA AAGGCACCGCCACCGATTTGAGGTGAACCCAGTCCTGAAAAAGTGCTTGGAAGAGCAAGGCTTGAAGTTC GTTGGCCAAGATGTGGAAGGCGAGAGGATGGAGATCGTGGAGTTGGAAGATCATCCATTTTTTGTTGGAG TGCAGTATCACCCCGAGTTCCTGTCCAGGCCTATCAAGCCCTCCCCACCCTACTTTGGCCTCCTCCTGGC CTCTGTGGGGCGGCTCCCACATTACCTTCAGAAAGGTTGCCGGCTCTCACCCAGGGACACTTACAGTGAC AGAAGCGGGAGCAGCTCCCCCGACTCGGAAATCACTGAACTCAAGTTTCCATCAATAAGTCAGGACTGAT CTGGATGAGTCTTCAACTTCAGCTTTGACAGTTTACAACTATGATTTTACATCCTGCTTTTGACACTTCT TTTAAATTATGTTTTTATTAAGATTATTTTATTATGGGGGAAAAGCATTTGGAAAACTTTGTCACTTGCA TGTCCCGTCATTTATACTTGATCCTGCAACACATGTATTTGTGCGACAATAATACCTTTGCAGTTTTTGT GCTTCTGTGATGGGAAACAGGACTCTGGATGGAACCTGATGGTGGCTGCTGGCTATGGTTAAGGGCCATC AACCACCTTGTTTTCCAGCCACTCGAGTCACTGTCATTGGAGTGAATTGCTGTTGTCTCCCTGGGGTGCC AGGACTCCCAGTGCTGGGCTGCTAACTGGGTGTTGCTGACATCTCAGTGCTGCTGAGTTACAGGGGACTA GGTGCCAGCTGTGGTTGCCCTCGCCATCCAGAGGGTTGTTTGGTTCTTTGGTACATCAAACTCTGTCTGT ATAAAGTATCATATAAAAACCCCGGGTCTCTGGCTACACTTTCTATTCCTGGGGCTGAGAAAACTCCTTG CATCAAGGGGTCAAGAAACACGGAATTTTAGGGTAACCGGTTCTAAAGCCCTCTCTTGTGTGTGCACCGT TGGCTTGAAGGTATTTTTTTTCTTCCAGAATGGTGTTATTGCATTTGAAATGCAATTTGAAGTTATTTTC TTAATGTGATATGGCAATGGGCATGACCATGTGCCTACAGTATGGTGGTTAGCTTGATGCATATCTAACT TAATAAATAATGCAGAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498717 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498717 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10934 same embryo
	EMAGE:10933 same embryo
	EMAGE:10938 same embryo
	EMAGE:10936 same embryo
	EMAGE:10937 same embryo
	EurExpress:euxassay_003471 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source