EMAGE mouse gene expression spatial database

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EMAGE:10938

Maoa monoamine oxidase A ( MGI:96915)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003370. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003370_01	euxassay_003370_02	euxassay_003370_03	euxassay_003370_04

euxassay_003370_05	euxassay_003370_06	euxassay_003370_07	euxassay_003370_08	euxassay_003370_09

euxassay_003370_10	euxassay_003370_11	euxassay_003370_12	euxassay_003370_13	euxassay_003370_14

euxassay_003370_15	euxassay_003370_16	euxassay_003370_17	euxassay_003370_18	euxassay_003370_19

euxassay_003370_20	euxassay_003370_21	euxassay_003370_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10938_wholemount_strong.wlz

10938_wholemount_moderate.wlz

10938_wholemount_weak.wlz

10938_wholemount_possible.wlz

10938_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10938_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal cortex

strong

regional

strong expression: see section 08 moderate expression: see section 09 10 17 18 19

vibrissa

moderate

regional

moderate expression: see section 04 19 20 weak expression: see section 02 03 05 06 21 22

olfactory lobe

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16 17

medulla oblongata

strong

regional

strong expression: see section 10 11 12 13 16 17 moderate expression: see section 09 14

metencephalon

strong

regional

strong expression: see section 11 12 13 16 17 18 moderate expression: see section 07 08 09 10 19 weak expression: see section 15

midbrain mantle layer

moderate

regional

moderate expression: see section 08 10 11 12 13 14 15 16 17 18 19 weak expression: see section 09

midbrain ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14 15 16 weak expression: see section 10

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 07 08 18 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 08 19 20

cervico-thoracic ganglion

strong

regional

strong expression: see section 10 11 12 16 17

cervical ganglion

strong

regional

strong expression: see section 08 09 10 16 17 18

thoracic ganglion

strong

regional

strong expression: see section 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 17 18 19

cochlea

moderate

regional

moderate expression: see section 05 06 07 19 20

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 21 22

lung

strong

regional

strong expression: see section 04 05 06 16 17 18 19 20 21 moderate expression: see section 07 08 09 10 11 12 13 14 15 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5915
Entity Detected:	Maoa, monoamine oxidase A ( MGI:96915)
Sequence:	sense strand is shown >T5915 TTTTACCCAAACCATGACGGATCTGGAGAAGCCCAGTATCACAGGCCACATGTTCGACGTAGTCGTGATT GGAGGCGGCATCTCAGGATTGGCTGCTGCCAAACTATTGTCAGAATACAAAATTAATGTCTTGGTTCTGG AAGCCCGGGATAGAGTTGGAGGAAGAACATATACTGTGAGGAATGAGCATGTTAAATGGGTAGATGTTGG TGGAGCTTATGTGGGACCAACCCAGAACAGAATCTTACGCTTGTCTAAGGATCTAGGCATAGAGACCTAT AAAGTGAATGTCAATGAGCGTCTAGTTCAATATGTCAAGGGGAAAACTTACCCATTCCGTGGTGCATTTC CACCTGTGTGGAACCCCTTGGCATATTTGGATTACAACAACCTGTGGAGGACAATGGATGATATGGGAAA AGAGATCCCTGTTGATGCACCATGGCAAGCAAGACATGCTGAGGAATGGGACAAGATAACCATGAAAGAT CTCATTGATAAAATCTGTTGGACAAAAACTGCTCGAGAATTTGCTTATCTTTTTGTGAACATTAATGTGA CTTCTGAGCCTCATGAGGTATCTGCCCTGTGGTTCTTGTGGTATGTGAGGCAGTGTGGAGGTACCTCTCG GATATTCTCAGTCACCAATGGCGGCCAGGAACGGAAATTTGTAGGTGGCTCTGGCCAAATAAGTGAACAG ATAATGGTCCTCCTTGGGGATAAAGTGAAGCTGAGCTCTCCTGTTACTTATATTGACCAAACAGATGACA ACATCATTATAGAAACACTGAATCATGAGCACTATGAGTGCAAATATGTAATTAGTGCCATCCCACCGGT TTTGACTGCCAAGATCCACTTTAAACCAGAGCTTCCACCTGAGAGAAACCAATTAATTCAGCGTCTTCCA ATGGGGGCTGTCATCAAGTGCATGGTGTATTACAAGGAAGCCTTCTGGAAGAAAAAGGACTATTGTGGCT GCATGATAATTGAAGATGAGGAGGCTCCAATTTCAATCACTCTGGATGACACTAAACCAGATGGGTCAAT GCCTGCCATCATGGGCTTCATACTTGCCCGGAAAGCTGAACGACTTGCTAAACTACATAAAGACATAAGG AAGAGGAAAATCTGTGAGCTGTATGCCAAAGTTCTGGGATCTCAAGAAGCTTTATCTCCCGTCCATTATG AAGAGAAGAACTGGTGTGAGGAGCAGTACTCCGGGGGCTGCTACACAGCCTACTTCCCTCCTGGTATCAT GACTCTGTATGGAAGGGTGATTCGGCAGCCAGTAGGCAGGATTTACTTTGCAGGCACAGAGACAGCAACA CAGTGGAGTGGCTACATGGAAGGTGCAGTTGAAGCTGGAGAACGAGCAGCTAGAGAGGTCTTGAATGCTC TAGGAAAAGTTGCCAAGAAGGATATATGGGTTCAAGAGCCTGAGTCCAAGGATGTTCCAGCTCTTGAAAT TACCCACACCTTCTTAGAGAGGAACCTGCCTTCCGTGCCTGGTCTGCTCAAGATCACTGGTTTTTCCACT TCTGTGGCTCTTCTCTGCTTTGTATTGTACAAGTTTAAACAGCCCCAGTCCTGAAGTTTTCACCCACAGG CCTCCTGCTTGATCATCCTTAGCACAATGAGCATGTAAAGGAAAGTGAGGATGAATTACAGCCTGTGGTT TGGGCAATTTAAGTGCACTTGATTTAACCCTGCTCAGTTTAATTTCAGTCTTTGTAAAGTGGTAACAGTC CAAAGAATCACCTAGCTAATTTCCATAAGAATGAGTTTCATCTTGTTTGTTAGACTAATTTGTGTTGGTT GATAGAATAAAATGTTGAGTGCTTAATTTCAAAAAGTTAAGTGCTTATGAGGAATAATTTCTGGATTCTG TTTTTTTTTTTCTTTTGCCTTTAATGCAAAACAGCTAATGGTTTCAGAAATAAAATAACTTGACTTACTG TGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3481702 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3481702 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10934 same embryo
	EMAGE:10933 same embryo
	EMAGE:10936 same embryo
	EMAGE:10935 same embryo
	EMAGE:10937 same embryo
	EurExpress:euxassay_003370 same experiment
	MGI:4826075 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source