EMAGE mouse gene expression spatial database

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EMAGE:10970

Gpaa1 GPI anchor attachment protein 1 ( MGI:1202392)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003340. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003340_01	euxassay_003340_02	euxassay_003340_03	euxassay_003340_04

euxassay_003340_05	euxassay_003340_06	euxassay_003340_07	euxassay_003340_08	euxassay_003340_09

euxassay_003340_10	euxassay_003340_11	euxassay_003340_12	euxassay_003340_13	euxassay_003340_14

euxassay_003340_15	euxassay_003340_16	euxassay_003340_17	euxassay_003340_18	euxassay_003340_19

euxassay_003340_20	euxassay_003340_21	euxassay_003340_22	euxassay_003340_23	euxassay_003340_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10970_wholemount_strong.wlz

10970_wholemount_moderate.wlz

10970_wholemount_weak.wlz

10970_wholemount_possible.wlz

10970_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10970_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6210
Entity Detected:	Gpaa1, GPI anchor attachment protein 1 ( MGI:1202392)
Sequence:	sense strand is shown >T6210 CAAGCTGCCGCGAGAGCAGAGCCCCGCGCCCCGCCATGGGTCTCCTGTCGGACCCGGTGCGCCGGCGCGC GCTCGCCCGCATCGTCTTGCGCCTTAACACACCTCTCTGTGTGCTGAGCTACGTGGCCGGGATCGCCTGG TTTCTGGCGCTGGCTTTTCCGCCGTTAACCCAGCGCACTTACATGTCTGAGAATGCCATGGGCTCCACCA TGGTGGAGGAACAGTTTGTAGGTGGAGACCGCGCTCGGAGCTTTGCCCGGGACTTCGCTGCCCACCGCAA GAAGCCGGGGGCTCTGCCAGTAGCCTGGCTGGAACGGTCCATGCGATCCGTAGGGCTGGAGGTCTACACC CAGAGCTTCTCCCGCAAACTACCCTTCCCGGATGAGACCCACGAGCGCTATATGGTGTCAGGTACCAACG TGTATGGCATCCTGCGGGCCCCACGTTCTGCCAGTACCGAGTCCCTGGTGCTTACTGTACCCTGTGGTCC AGATGCCACCAATAGCCAGGCTGTGGGGTTGTTGTTGGCACTTGCTGCCCATTTCCGAGGGCAGATTTAC TGGGCCAAAGATATCATTTTCCTGGTAACAGACCATGATCTTCTGGGCACCGAGGCTTGGCTCGAAGCCT ACCATGACATTAATGTTACAGGCATACAGTCATCTCCCCTGCAGGGCAGGGCTGGAGCCATCCAGGCTGC TGTAGCTCTGGAGCTGAGCAGCGACGTGGTCACCAGCCTCGATGTGACAGTGGAGGGACTCAACGGTCAG CTGCCCAACCTTGATCTGCTCAACCTCTTCCAGACCTTCTGCCAGAAAGGGGGGCTGTTGTGTACACTCC AGGGCAAGCTGCAGCCCCAGGACTGGACGTCACTCGAAGGGCCGTTGCAGGGTTTGCAGACACTGCTACT CATGGTGCTGCGGCAGGCCTCTGGCCGGCCCCACGGCCCCCACGGCCTTTTTCTGCGCTACGGAGTGGAG GCACTAACCCTACGCGGCATCAATAGCTTCCGCCAATACAAGTATGATTTGGCGACAGTGGGCAAGGCTC TGGAGGGCATGTTCCGCAAGCTCAACCACCTCCTGGAGCGCCTGCACCAGTCATTCTTCTTCTACCTGCT GCCGGCCCTCTCCCGCTTTGTCTCCATCGGACTCTACATGCCCGCCACGGGCTTCCTGCTCCTAGTCCTT GGACTCAAGGCTCTGGAGCTGTGGATGCAGTTACACCAGGCTGGAGTCAACCCGGAGGAGGCTGGGAAGG CCCCTAGTCCCGGGACTCCACTCTTGCCAACACAGGGTGTGGGCCTGGCCTCACTTACGGCACCCCTGCT GATCTCTCAGGCCATGGGTCTGGCCCTCTATTTCCTGCCTGTGCTGGGCCAACATTTAGCTACTCAGCAT TTCCCAGTGGCCGAAGCCGAGGCTGTTGTGTTGACGCTGCTCGCGATTTATGTAGCCGGCCTGGCCCTTC CACACAACACCCACCGAGTGGTCAACTCACAGGTCCCAGACAGGGGCTGGATGGCACTGAAACTAGTGGC CCTCATCTACCTGGCACTGCAGCTGGGTTGCATTGCTCTCCTCAATTTCTCCCTGGGCTTCCTGCTGGCT GCCACCATGGTCCCTGCTGCTGCACTCGCCAAGCCCCATGGGCCCCGGACACTGTATGCTGCCCTGCTGG TAGTGACCAGCCCAGCAGTCACACTCTTCGGTAGCCTGTTCCTGTGGCGGGAGCTGCTGGAGGTACCGCT GTCTCTGGCAGAGGGTTGGCAGCTCTTCCTCACAGCATTGGCCCAGGGTGTGCTGGAACACTACACCTAT GGCGCCCTCCTCTTCCCAATACTGGCCTTGGGTCTGTATCCTTGCTGGCTGCTTTTCTGGAATGTCCTCT TCTGGAAGTGAGGTGTCTGGGAAGGGGTCCCCTAAGCCTCCGTTCTACCTCCTCCCTGGGAAATAAACAA GTGCTGTGTTTCTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498573 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498573 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10974 same embryo
	EMAGE:10969 same embryo
	EMAGE:10973 same embryo
	EMAGE:10971 same embryo
	EMAGE:10972 same embryo
	EurExpress:euxassay_003340 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source