EMAGE mouse gene expression spatial database

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EMAGE:10973

Ube4a ubiquitination factor E4A, UFD2 homolog (S. cerevisiae) ( MGI:2154580)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003317. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003317_01	euxassay_003317_02	euxassay_003317_03	euxassay_003317_04

euxassay_003317_05	euxassay_003317_06	euxassay_003317_07	euxassay_003317_08	euxassay_003317_09

euxassay_003317_10	euxassay_003317_11	euxassay_003317_12	euxassay_003317_13	euxassay_003317_14

euxassay_003317_15	euxassay_003317_16	euxassay_003317_17	euxassay_003317_18	euxassay_003317_19

euxassay_003317_20	euxassay_003317_21	euxassay_003317_22	euxassay_003317_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10973_wholemount_strong.wlz

10973_wholemount_moderate.wlz

10973_wholemount_weak.wlz

10973_wholemount_possible.wlz

10973_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10973_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 11 12 13 14 15

vibrissa

moderate

regional

moderate expression: see section 05 20 21 weak expression: see section 06 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 20 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 17 18 19

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 16 17

cervical ganglion

moderate

regional

moderate expression: see section 09 17

thoracic ganglion

moderate

regional

moderate expression: see section 13 14

dorsal root ganglion

strong

regional

strong expression: see section 17 18 moderate expression: see section 09 10 11 12 16 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6203
Entity Detected:	Ube4a, ubiquitination factor E4A, UFD2 homolog (S. cerevisiae) ( MGI:2154580)
Sequence:	sense strand is shown >T6203 GTAGCTGTGAGGTGTCGTCGTCCCTGCCCTTTCAGGCTCCGGTACTTTTATTTTCATGTGTAAGCTCAAT GACTAAGTCTAACCTGGATCTCCACCGAGGTTCTCAACAGCCGCTCCCCTAGGGCTGGATGGAGGACACC TTAAAGTGAAATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGC TCCCTGGCTGATGCCAAGCAGTTTGCAGCAATCCAGAAAGAGCAGCTGAAGCAGCAGTCCGATACCTTAT CAATCTCATCTTTTGTATCAAGAGGACATCCGTGCGTCCAGACTCCCTTTGGCCCAGTGGCACCTATTGC TACATCAGTGACAGAATCAGGAGTCACCCATCTCAGGATCATGAGGAAGAGATAGATGGGCACAGCCTAT AAAGCAAAACTAGCCTGTAACTGGACCACAGTGAGGTAGTGTATTTGGTTCTGAGGAAGAAGCGTTGTGT AACAGCTTACATGTTCCAGCAGATGAGCTCCCAGCAAGCCCAGATGACTCGGATAATAGTGTGTCAGAGA GCCTGGATGAGTTTGACTACTCTGTATCTGAGATTAGCCGCTCCTTCCGGACACACCAAGAAATGTGTGA GCAACTCAACATCAATCACATGATCCAGAGGATCTTCCTCATCACCCTGGACAACAGTGATCCAAGCTTG AAAAGCGGGAATGGCATCCCCAGCCGTTGTGTGTACTTGGAAGAAATGGCAGTAGAGCTGGAAGATCAAG ACTGGCTTGATATGAGCAATGTTGAGCAGGCCATCTTTGCTCGATTATTGCTTCAAGATCCAGGAAACCA CTTGATCAGCATGACTTCCTCCACAACTTTAAATCTCTCTGCTGATCGTGATGCAGGAGAGAGGCACATC TTCTGTTACCTTTACTCCTGCTTCCAGAGAGCCAAGGAAGAGATTACCAAAGTTCCAGAAAACCTACTCC CCTTTGCAGTGCAGTGCAGAAACCTCACTGTGTCCAACACTCGAACAGTTCTCCTCACCCCAGAGATCTA TGTTGACCAAAACATCCATGAGCAACTGGTAGATTTGATGTTGGAAGCTATTCAGGGAGCCCATTTTGAA GATGTAACTGAATTTTTAGAAGAGGTCATTGAAGCCTTGCTGTTGGATGAGGAGGTTCGAACATTTCCTG AAGTCATGATTCCAGTGTTTGATATTTTACTCAGCCGAATAAAAGATCTGGAACTCTGCCAGATCCTCCT GTATGCATATCTGGACATTCTTCTCTATTTCACTAGGCAGAAGGATATGGCCAAGGTGTTTTTAGAATAC ATTCAGCCCAAGGATCCCAGCAATGGGCAGATGTATCAGAAGACCTTGCTGGGAGTCATCCTGAATATCT CGTGCTTGTTAAAGACTCCGGGTGTGGTAGAGAACCATGGCTTCTTCCTGAATCCATCCCGTTCCAGTCC TCAGGAGATCAAAGTACAGGAGGCCAACATCCATCAGTTCATGGCTCAGTTCCATGAAAAGATCTACCAG ATGCTGAAGAACTTGCTCCAGCTCTCTCCAGAAACCAAGCACTGTATCTTGTTCTGGCTTGGAAACTGTT TGCATGCAAATGCAGGCCGCACCAAGATCTGGGCCAATCAGATGCCCGAAATCTTCTTCCAAATGTATGC CTCGGATGCCTTCTTTCTGAATCTGGGTGCTGCCCTCCTGAAGCTGTGCCAGCCATTCTGCAAGCCCAGA TCCTCCCGGCTCCTCACCTTTAACCCCACGTACTGTGTCCTCAAGGACTTGAATGATGAAGAACGAAAAA TTAAAAGTGTGCATATGAGAGGTTTGGACAAGGAGACCTGTCTGATCCCAGCTGTGCAGGAGCCAATGTT CCCCCAGAGCTACAACCTTGTGACGGAGAACCTGGCCCTGACAGAGTATACCTTGTACTTGGGATTTCAC AGGTTGCATGATCAGATGGTAAAAATCAACCAAAATCTGCATCGGCTGCAGGTTGCCTGGCGGGATGCTC AGCAGAGTTCTAGCCCTGCTGCTGACAATCTCCGTGAGCAGTTCGAACGCCTGATGACCATCTATCTCTC TACCAAGACTGCTATGACTGAACCCCAGATGCTGCAAAACTGTCTGAATTTGCAGGTGTCCATGGCTGTT CTGCTGGTTCAACTGGCCATAGGCAATGAGGGCTCCCAGCCAATAGAACTAAGTTTCCCTTTGCCAGATG GCTACAGCTCTTTGGCTTATGTGCCAGAATTTTTTGCAGATAACTTGGGTGATTTCCTCATTTTCCTCCG GCGCTTTGCTGAGGACATCTTGGAGACGTCAGCAGACTCCCTGGAGCATGTCCTTCATTTTATCACCATT TTCACTGGAAGCATAGAAAGAATGAAGAATCCTCACCTGAGGGCCAAACTAGCAGAGGTGTTAGAAGCAG TGATGCCTCACCTAGACCAGACCCCAAGCCCCTTGGTGTCCAGTGTGTTCCACCGGAAACGTGTGTTCTG CAACTTTCCATACGCTCCCCAACTTGCAGAAGCTTTGATCAAGGTGTTTGTGGACATCGAGTTTACAGGG GACCCCCATCAATTTGAACAGAAGTTTAATTACCGCCGTCCTATGTATCCCATCCTAAGATACATGTGGG GGACAGATTGCTATCGGGAGAGCATTAAGGATTTGGCTGACTATGCCTCTAAGAATTTAGAAGCTATGAA TCCCCCACTGTTCCTCCGATTTCTTAACCTGTTAATGAATGATGCTATCTTCCTTTTGGATGAAGCCATA CAGTATTTGAGCAAGATAAAGATTCAGCAGATTGAGAAAGACCGAGGAGAATGGGAGAGTCTGACACCAG AAGCCCGCCGAGAGAAAGAGGCAGGTCTGCAGATGTTTGGGCAGCTGGCACGGTTCCATAACATCATGTC CAATGAAACAATTGGTACCCTTTCCTTCCTGACATCAGAGATCAAGTCGCTGTTTGTGCATCCGTTCCTG GCCGAGCGCATCATCTCCATGCTCAACTACTTCCTGCAGCACCTGGTGGGCCCCAAGATGGGTGCCCTCA AAGTCAAGGACTTCAGCGAATTTGACTTCAAACCTCAGCAGCTCGTATCAGACATCTGCACTATCTACTT AAATCTTGGGTATTTGAGATTGGTGTTGAGAAAGCCATAGGGATGCTAGTATTTTGATAGCAGAATGACT GGTTAGGTCTTGTGTGAAAATAGTGGGGGAAGGCAGGCTCATTTCTAGCTGATAGATAAAGGACTCCTCC ACAGTACTAAATAGATTTTATCTGGCCCATTGGACATTGCTCAGTATTTCCAACATTCTGCCTTGATCTG TGCAAAGATAAATATTACTGGTTATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498834 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498834 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10974 same embryo
	EMAGE:10969 same embryo
	EMAGE:10971 same embryo
	EMAGE:10970 same embryo
	EMAGE:10972 same embryo
	EurExpress:euxassay_003317 same experiment
	MGI:4829060 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source