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EMAGE:10975

Tmem150a transmembrane protein 150A ( MGI:2385244)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10975 EMAGE:10975 EMAGE:10975 EMAGE:10975 EMAGE:10975
"Pseudo-wholemount" of euxassay_001192. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001192_01 euxassay_001192_02 euxassay_001192_03 euxassay_001192_04
EMAGE:10975 EMAGE:10975 EMAGE:10975 EMAGE:10975 EMAGE:10975
euxassay_001192_05 euxassay_001192_06 euxassay_001192_07 euxassay_001192_08 euxassay_001192_09
EMAGE:10975 EMAGE:10975 EMAGE:10975 EMAGE:10975 EMAGE:10975
euxassay_001192_10 euxassay_001192_11 euxassay_001192_12 euxassay_001192_13 euxassay_001192_14
EMAGE:10975 EMAGE:10975 EMAGE:10975 EMAGE:10975 EMAGE:10975
euxassay_001192_15 euxassay_001192_16 euxassay_001192_17 euxassay_001192_18 euxassay_001192_19
EMAGE:10975 EMAGE:10975 EMAGE:10975 EMAGE:10975 EMAGE:10975
euxassay_001192_20 euxassay_001192_21 euxassay_001192_22 euxassay_001192_23 euxassay_001192_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10975Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10975_wholemount_strong.wlz
10975_wholemount_moderate.wlz
10975_wholemount_weak.wlz
10975_wholemount_possible.wlz
10975_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10975_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
liver lobe
weak weak
single cellweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4876
Entity Detected:Tmem150a, transmembrane protein 150A ( MGI:2385244)
Sequence:sense strand is shown

>T4876
CGGACGCGTGGGCGAGGGGGCCGAGCAGGGAGAGGCGGCAGCGGCGGCGGGGAGCTCCGGGGAGGCCAGG
GCAGCTGATGATTGTGACCGAAATATCTCAAAGTAGCTGGGCCTGCCCCCTTTTCCCCACCTACCTGTTG
TCCTTACCCCCAAACTACCACCACCCTGGCTCCACTCCCTCATGACCGCCTGGATCCTCCTTCCTGTCAG
CCTGTCAGCCTTCTCCATCACCGGCATATGGACTGTGTATGCCATGGCCGTGATGAACCGCCACGTGTGC
CCAGTGGAGAACTGGTCCTACAATGAATCCTGCTCTCCTGACCCTGCTGAACAAGGGGGCCCTAAGAGCT
GCTGCACCCTGGATGATGTTCCTCTCATCAGCAAGTGTGGCACATACCCCCCGGAGAGCTGCCTCTTCAG
CCTCATTGGCAACATGGGTGCTGTTATGGTGGCCCTCATCTGCCTCCTTCGGTACGGGCAGCTCCTGGAA
CAGAGCCGGCACTCCTGGATCAATACCACTGCACTCATCACTGGTTGCACCAACGCTGCAGGCCTCGTGG
TGGTCGGCAATTTTCAGGTGGACCATGCCAAGTCTCTACACTACATCGGAACTGGTGTGGCCTTCACTGC
TGGGCTGCTCTTTGTGTGCCTGCACTGTGTTCTCTTCTACCATGGGGCCACCACCCCCCTGGACATGGCT
ATGGCCTACCTTCGAAGTGTGCTGGCTGTCATCGCCTTCATCACCCTGGTCCTTAGTGGAGTCTTCTTCC
TCCACGAGAGTTCTCAGCTCCAGCATGGAGCTGCTCTGTGTGAATGGGTGTTTGTCCTCGATATCCTCAT
TTTCTATGGCACCTTCAGCTATGAGTTCGGGACAATCTCCTCAGACACACTGGTGGCCGCACTACAGCCT
GCCCCGGGCAGGGCCTGTAAGTCCTCTGGGAGCAGCAGCACCTCTACCCACCTCAACTGTGCTCCCGAGA
GTATTGCCATGATCTGAGGTCTGGAGGGTGGCTGGCCCAGCCTCCAAAACACCCGCACCCTCTACCTTCT
TTGCATTTATTTTGTACCAAAAACAAGTTTTAGAGAGTATTGTTGGGATCTAGGCTTCCTTACTTCTGGA
GAAGTGGCCATCCAACACCCACCTGTGCCATAGAGGGACAGGGCCTCCACAGCTGCCTCTGCTGCTTGTC
CTGTCCCCTCATACCAGAGTAGTATTCTGTGTTAAAGGTCACCTGTCCTCCCTTGCCCAGCCAGCCCTTC
AGGTGCCTTCTACTCCCAGTGCCAGAGCCAGACCACTGGGGCCTTCTGCTGCAGGAATTGGGGCTGGGGT
GGCAGGGGCTCACGAGTCTGGATGATGGGAGGAGCATGCCTTAGCCTAAGGGAAGGCCCACCCAGCTGCA
CTTGGGTTCTTCACTCTGCCCCTCACTTCCTTTAGGGCAAATAACACAGCAGAACCACACGAGTATTTTA
GTACTTTTTTATATCTATTAAAAGAATTCTAATTTGCACGTCAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5097962 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5097962 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10976 same embryo
 EMAGE:10978 same embryo
 EMAGE:10979 same embryo
 EMAGE:10977 same embryo
 EMAGE:10980 same embryo
 EurExpress:euxassay_001192 same experiment
 MGI:4828779 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS