EMAGE mouse gene expression spatial database

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EMAGE:10987

Fam26e family with sequence similarity 26, member E ( MGI:2143897)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001191. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001191_01	euxassay_001191_02	euxassay_001191_03	euxassay_001191_04

euxassay_001191_05	euxassay_001191_06	euxassay_001191_07	euxassay_001191_08	euxassay_001191_09

euxassay_001191_10	euxassay_001191_11	euxassay_001191_12	euxassay_001191_13	euxassay_001191_14

euxassay_001191_15	euxassay_001191_16	euxassay_001191_17	euxassay_001191_18	euxassay_001191_19

euxassay_001191_20	euxassay_001191_21	euxassay_001191_22	euxassay_001191_23	euxassay_001191_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10987_wholemount_strong.wlz

10987_wholemount_moderate.wlz

10987_wholemount_weak.wlz

10987_wholemount_possible.wlz

10987_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10987_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 13 17 weak expression: see section 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4703
Entity Detected:	Fam26e, family with sequence similarity 26, member E ( MGI:2143897)
Sequence:	sense strand is shown >T4703 AGGCAAACGTCTTTCCCTCTTCGCGTGTGTGTGTGTGTGTGTGTGTGTGTCTCCAACATGGATGCCTTTC AGAGCATCTTAAAATTCTTTCTTAACCAAAAAACTGCTATTGGCTACAGCTTCATGGCTTTGCTCACCGT GGGTAGCGAACGTCTCTTCTCGCTGGTGGCATTTAAGTGCCCCTGCAGCGTTGAGAATACTGCCTATGGG CTGGTTTTTCTCTTCGCCCCTGCCTGGGTGTTACTGATCCTTGGCTTCTTTCTGAATAACAAGGCATGGA GACTCTTCACTGGCTGCTGTATGAACCCAAAAAAAATATTTCCCAGGAGACGCTGCTGCCGCTTCTTCTA CGTCCTGGGCCATATCATACTGAGTTCACTGGTGGCTCCGGTGATGTGGCTCTCTGTGGCTTTGCTTAAT GGGACGTTTTATGAATGTGCTATGAGCGGGACAAGAAGCACAAGGCTCCTGGAGATGATTTGCAAGGGCA AGCCCAAAGAGTGCTGGGAAGAACTGCACAAAGTCTCCTGTGGTAAAAGTAGCATGGCCGCTATGGAGAG TGAAGAAGTGAGGCTATCCCTGCAGGCCCAGTCTCAGATCCTAGGATGGTGCCTGATTTGTTCGGCGTCC TTCCTCTCCCTGCTGACCACTTGTTATGCACGCTGCAGATCTAAAGTCAGCTATCTGCAGCTGAGTTTTT GGAAGACATATGCACAAAGGGAGAAGGAGCAACTGGAAAACAAACTCCTGGAATGTGCTAACAAGTTAAG TGAGAGGAACCTCAAATGCTTTTTTGAAAACAAGAAGCCAGATCCCTTTCCCATGCCCTCATTTGGTGCC TGGGAGGCTGCATCCGAGCTACACTCCTTCCATCAAGACCGTGAGCACTACAGCACCCTCCACAAAGTGG TAGATGATGGTATGGAACAAACCCCGCAAGAGGAAGAAACAACAATGATCCTTGTGGGTACTGCCCAGAG TTTGTGAATCATCTACCATTCCTGGCTCAGATATCTAGTGGTACACTCATTCGGAGTTGAGTTTTCTGCT GTTTCCATGACAACCTGAGCCTCTGCATTTTCTCACCATCAGAGCTTCTTTGGAAGATAACACAGTGGGA AACTGTTCTGAAGCCTCGAAGAGCAATGTCTGGATGTGCTCAGACTGATTGATGCTAAGTGATGACAGAC ACTTTGAGCTGGTGGAGGGTTGTGTCCAGGAGGTCCAACAGTTGATCCAAGCTCTTAAGATTTTATAACT CAGCCCTTAGAGTCTGGCAAGATGGTCTGCCACTATTTGTAAGCATCTGTCATGCTATTCTGGGAGAGGT CTGTGCCCAGTGGTATCCACATAGTGGCCATCAGGAACCTTTCAGAAATATATCCAACACCTTTTAAGTT GGAAGGAATTCTGCCATTTAATTAACATTCCTTTCTATAGAATACCTCTATTCCCAAGACTCACTAAATT GAAAGAATATAATATCTAAAAATCAGAGGTTCCTGCATTTACCCCAATTTCTGAAAGTCTGCCTGGAAGA GGGCTGCCACATAACTGCTGTGGGTGATAGGTACTTATACCATCACAGGACAATTTCTACATTCAAAAGA CAATCACATAAGGCATAATACAAGACTGCATTGATATAAAGACAAGTATGTTAATATTGTATATTAAAGT AGTTTTTCACAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5031155 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5031155 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10990 same embryo
	EMAGE:10988 same embryo
	EMAGE:10986 same embryo
	EMAGE:10985 same embryo
	EMAGE:10989 same embryo
	EurExpress:euxassay_001191 same experiment
	MGI:4824740 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source