EMAGE mouse gene expression spatial database

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EMAGE:11005

Cyp2j6 cytochrome P450, family 2, subfamily j, polypeptide 6 ( MGI:1270148)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001040. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001040_01	euxassay_001040_02	euxassay_001040_03	euxassay_001040_04

euxassay_001040_05	euxassay_001040_06	euxassay_001040_07	euxassay_001040_08	euxassay_001040_09

euxassay_001040_10	euxassay_001040_11	euxassay_001040_12	euxassay_001040_13	euxassay_001040_14

euxassay_001040_15	euxassay_001040_16	euxassay_001040_17	euxassay_001040_18	euxassay_001040_19

euxassay_001040_20	euxassay_001040_21	euxassay_001040_22	euxassay_001040_23	euxassay_001040_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11005_wholemount_strong.wlz

11005_wholemount_moderate.wlz

11005_wholemount_weak.wlz

11005_wholemount_possible.wlz

11005_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11005_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trigeminal v ganglion

weak

homogeneous

weak expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

vestibulocochlear viii ganglion

weak

homogeneous

weak expression: see section 06 07 08 09 18 19

nasal capsule

weak

regional

weak expression: see section 08 09 10 11 12 17 18 19 20 21

esophagus

weak

regional

weak expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1052
Entity Detected:	Cyp2j6, cytochrome P450, family 2, subfamily j, polypeptide 6 ( MGI:1270148)
Sequence:	sense strand is shown >T1052 CCTCNANNCTGTTGGCCTACTGGAGAAGGGTGCCCTTGTTGTTAGCACCTGGGACTTGCCGCAGCTCAGA CCTTCATTCCTCAGACGAACCAGCTGCAGTCCTAGCCATGCTCGCTGCTACCGGCTCCTTGTTAGCCACG ATCTGGGCAGCGCTCCATCCCAGGACTCTGTTGGTGGCTGCAGTCACCTTCCTGCTTCTGGCTGATTACT TCAAAAACCGGCGCCCCAAGAACTATCCCCCAGGGCCTTGGGGTCTGCCTTTCGTGGGCAACATATTCCA GTTGGACTTTGGGCAGCCCCACCTCTCAATCCAGCCGTTGGTGAAGAAATATGGGAATATTTTTAGCCTG AATCTTGGTGACATAACTTCAGTGGTCATAACTGGACTGCCCTTAATCAAAGAAGCACTTACTCAAATGG AACAAAACATTATGAATCGTCCTCTAAGTGTTATGCAAGAACGTATATCTAATAAAAATGGATTGATCTT CTCCAGTGGCCAAATATGGAAGGAGCAAAGAAGGTTTGCCCTGATGACACTGAGGAACTTTGGACTGGGA AAGAAGAGCT
Notes:	The probe template was PCR amplified from IMAGE:2088372 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2088372 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11004 same embryo
	EMAGE:11003 same embryo
	EMAGE:11002 same embryo
	EMAGE:11006 same embryo
	EurExpress:euxassay_001040 same experiment
	MGI:4824173 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source