EMAGE mouse gene expression spatial database

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EMAGE:11073

Hmgn1 high mobility group nucleosomal binding domain 1 ( MGI:96120)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003511. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003511_01	euxassay_003511_02	euxassay_003511_03	euxassay_003511_04

euxassay_003511_05	euxassay_003511_06	euxassay_003511_07	euxassay_003511_08	euxassay_003511_09

euxassay_003511_10	euxassay_003511_11	euxassay_003511_12	euxassay_003511_13	euxassay_003511_14

euxassay_003511_15	euxassay_003511_16	euxassay_003511_17	euxassay_003511_18	euxassay_003511_19

euxassay_003511_20	euxassay_003511_21	euxassay_003511_22	euxassay_003511_23	euxassay_003511_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11073_wholemount_strong.wlz

11073_wholemount_moderate.wlz

11073_wholemount_weak.wlz

11073_wholemount_possible.wlz

11073_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11073_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 03 04 05 06 07 08 19 20 21 22 23

cerebral cortex mantle layer

strong

regional

strong expression: see section 02 03 04 05 18 19 20 21 22 23 24

cerebral cortex marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex marginal layer

strong

regional

strong expression: see section 09 10 11 14 15 16

cochlea

strong

regional

strong expression: see section 05 06 07 08 09 18 19 20 21 22

kidney calyx

moderate

regional

moderate expression: see section 18 19 weak expression: see section 07 08 09 10 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7926
Entity Detected:	Hmgn1, high mobility group nucleosomal binding domain 1 ( MGI:96120)
Sequence:	sense strand is shown >T7926 GTGGCGGCGGCGGCTTGGCAGTGCGGCTCCTCGGTGACAGATCCGACGACGCACGCGTCTCCCACCCCGC GCCGCCACGATGCCCAAGAGGAAGGTTAGCGCGGATGGAGCGGCCAAGGCGGAGCCCAAGCGCCGCTCCG CGAGGCTGTCGGCCAAGCCCGCCCCTGCCAAGGTGGACGCGAAGCCGAAAAAGGCCGCGGGAAAGGATAA AGCATCAGACAAAAAAGTGCAGATAAAAGGGAAGAGGGGAGCGAAGGGCAAACAGGCTGACGTGGCTGAC CAGCAAACCACAGAGCTGCCTGCAGAAAATGGAGAGACGGAAAACCAGAGTCCAGCCTCTGAAGAAGAGA AAGAAGCTAAGTCCGACTAAGCATCCATCACGTCTGTCAGTGGTCCCGCCTCCCTTCTTGTACAATCCAG AGGAATATTTTTATCAACTATTTTGTAAATGCGAGTTTTTTAGTAGCTCTAGAAACATTTTTAAAAGGTG AGGGGATCCCACCTCATCCCATTTTTTAAGTGTAAATGGTTTTTTTAAGAGGTTAAATCATTTGCTGGTT GTTTATTTTTTGGTACAACCAGAAAATAGCAGGATACTGAATCAGGAGAGGCTGTGACTGTCTCGGGGGT CACCATAACATTCCGTAGATGGGGCTAGTTTTATATCCTATAATACAAAGTATACTAAATGGCAATTTGG CGTCTCAGTCGTGCATTCGTGTCTGAAATATTTTCAGTTCTATCTGGTCCCGTGTTTCTAGTAGAACCAT TTTGTAAGAAAGCCAGTCCTTGGCCCTTGCCCTGTCAGAACTGTGATGTCTGTGGTCATGGCAGTCCATT TTCCTAACAGTTGTGATAATGTGCTGTGAAAGATGGAAGTCCTGAGTCTGTTCTGTATGTGGCGTAACAT TGTGAATTGGTGGGACTGAGGATTACAGGCATTTGGTTGTTGTGAGAGGTCTTGGGGGAACTTGCCGAGT CACTGGAACAAGTTCAAAGAAAAACAATACGTAGCTCTTCATTTGTGTACATGAACACAATGTGACTTCA GAGTTTGGGTACGTGAACACAGTATATGGCTCTTTTTGGGTACATGTAATAAAAATTGGAATGTCTGTAC TCTGCTCCTCGACCAATAAAATCTGTTGTGAAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6515849 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6515849 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11074 same embryo
	EMAGE:11075 same embryo
	EMAGE:11076 same embryo
	EurExpress:euxassay_003511 same experiment
	MGI:4825395 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source