EMAGE mouse gene expression spatial database

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EMAGE:11076

Alx1 ALX homeobox 1 ( MGI:104621)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003530. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003530_01	euxassay_003530_02	euxassay_003530_03	euxassay_003530_04

euxassay_003530_05	euxassay_003530_06	euxassay_003530_07	euxassay_003530_08	euxassay_003530_09

euxassay_003530_10	euxassay_003530_11	euxassay_003530_12	euxassay_003530_13	euxassay_003530_14

euxassay_003530_15	euxassay_003530_16	euxassay_003530_17	euxassay_003530_18	euxassay_003530_19

euxassay_003530_20	euxassay_003530_21	euxassay_003530_22	euxassay_003530_23	euxassay_003530_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11076_wholemount_strong.wlz

11076_wholemount_moderate.wlz

11076_wholemount_weak.wlz

11076_wholemount_possible.wlz

11076_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11076_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7917
Entity Detected:	Alx1, ALX homeobox 1 ( MGI:104621)
Sequence:	sense strand is shown >T7917 GTCTGGGAGCTGGGCAGCTCCTGGCGTCGCAGGTCTCGCTTCCCCTTTCCCTCCTCTCTGACCCTCCCTA CTCCCGCCCACTGCTCCCTCCTCCCTCCGCGCCCTCCAGCCTCCGCACGCCCCGGAGAGCTCGCGGACAG CCTTTCCAGGATTATGGAGTTTCTGAGCGAGAAGTTTGCCCTGAAGAGCCCGCCAAGTAAAAACAGTGAC TTTTACATGGGCACAGGAGGCGCGTTGGAGCACGTTATGGAGACGCTGGACAATGAGTCCTTTTACGGCA AAGCGACGGCTGGCAAATGCGTGCAGGCCTTCGGGCCGCTGCCGCGAGCGGAGCATCACGTGCGCCTGGA CAGGACTTCGCCCTGTCAGGACAGCAGCGTGAACTATGGAATCACCAAAGTGGAAGGACAGCCCCTTCAC ACCGAGCTGAATAGAGCAATGGATAACTGCAACAATCTCAGGATGTCTCCCGTGAAAGGGATGCCAGAGA AGAGCGAACTGGATGAACTTGGGGACAAATGTGACAGCAATGTATCCAGCAGCAAAAAGAGGAGACACCG AACTACCTTTACCAGTTTGCAGCTGGAGGAACTGGAGAAAGTTTTCCAGAAAACCCATTACCCAGATGTA TATGTCAGAGAACAACTTGCCCTGAGAACGGAGCTCACTGAGGCCAGGGTCCAGGTTTGGTTTCAAAATC GAAGGGCCAAGTGGAGAAAAAGAGAACGATACGGCCAAATACAGCAAGCTAAAAGCCATTTCGCTGCCAC GTACGACATATCAGTCTTACCAAGGACGGACAGCTACCCACAGATCCAGAACAATTTGTGGGCAGGAAAT GCTAGCGGTGGTTCTGTGGTTACGTCATGCATGCTACCACGTGACGCGTCCTCCTGCATGACACCTTACT CTCACTCGCCTCGGACAGATTCCAGTTATACGGGGTTTTCGAACCACCAGAATCAGTTCAGCCACGTGCC CCTCAATAATTTTTTCACTGACTCTCTTCTCACTGGAGCTACCAACGGACACGCATTTGAAACAAAGCCG GAGTTTGAAAGGAGGTCTTCCAGTATCGCTGTTCTGAGAATGAAGGCCAAGGAGCACACCGCCAATATTT CATGGGCCATGTAACATACAGTACTCTTTTATTTTTCTTTTCATAGCAAAGTAAAACATTCTTATTTCTC GTATTTAAAGGATACCACAATAAGCTGCTGTGTGTGGAAGGGCTAAAGGTCAAGATATACGGTGAGACCA GCTTACATGAATAGTTGTTATTATTTAACATTTAAATCTAAGAATGGATCCCTGAAAGGACTAAATAGGT TTACCATGCACCAGCCTCCACAGACTCTGTTTTAGTAGTAAGTTTTTTGGTTTTTTTTTTTTTCCTATTG TACAAGTCAATGAAATATGGTCAGGCAACTTCTTAAAGGAATAAATGTATTAAACAAATCCTTCTGTGAT AGATTGATTTATGCTTGTTCTGTATAACAAGAAATCCCTTGTAAAGTGATACCTGATAAACGAGGAAACA CCCCGGGGGGAAAAAGGCAGTATTGATACAGGGGGCTTTGAGAGCTAATGCCATTTTCAATTCTTTGGCT CCAAGACACAATGGTCATACACTCTGTTGCAGCCTCAAGATTCTTTTCTTTTAACCTTTCAATGTTTTGG TGAATTTCCACTCAGTCGGAAAAAAAAGTCCTTCTGAACTGCTCTGTATTTGCATGGATATCTGGGCACA GTTTCTTGCAACCATGTGGTTAAAGGTGACAACTGAAGTATTTGCGTATGATTTAAAGTCTAGTCAACTG GACAGGATTTATAAATCCAACTTTCAATTGAAGAGGCCAGCAGGCAGACTGCAATTTTGTGATTAAAACT GAGAACTCATTAGTAAACGTGCTGTTGCTTTTATACTAAAATCATGTTGATTCAATCGCATACAAACGTA TCTTGCAAATAAAGGGTAACTTTTCGATTAAAGGAGTAACAGATTGGAATTTATGTTTGTTTTGTGGGCC TATGCATGTTCAAATGCATGCCTGTTTGGCCTTGACACTCTGTGCCTTAAGCATTTTTGCTACTGTGCTA TCCATAGAGAATTAAAATGTCAGGAGGTGCTCTAGAATTTCTTGTTAAAAATAAAAAGAAGAAGACAAAT AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6513129 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6513129 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11074 same embryo
	EMAGE:11075 same embryo
	EMAGE:11073 same embryo
	EurExpress:euxassay_003530 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source