EMAGE mouse gene expression spatial database

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EMAGE:11099

Ccdc53 coiled-coil domain containing 53 ( MGI:1914532)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003569. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003569_01	euxassay_003569_02	euxassay_003569_03	euxassay_003569_04

euxassay_003569_05	euxassay_003569_06	euxassay_003569_07	euxassay_003569_08	euxassay_003569_09

euxassay_003569_10	euxassay_003569_11	euxassay_003569_12	euxassay_003569_13	euxassay_003569_14

euxassay_003569_15	euxassay_003569_16	euxassay_003569_17	euxassay_003569_18	euxassay_003569_19

euxassay_003569_20	euxassay_003569_21	euxassay_003569_22	euxassay_003569_23	euxassay_003569_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11099_wholemount_strong.wlz

11099_wholemount_moderate.wlz

11099_wholemount_weak.wlz

11099_wholemount_possible.wlz

11099_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11099_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6710
Entity Detected:	Ccdc53, coiled-coil domain containing 53 ( MGI:1914532)
Sequence:	sense strand is shown >T6710 CGGACGCGTGGGTGAGCCCCGGGGTCCCCATCCGCTGAGGAGATGGATGAGGATGGGCTTCCTCTCATGG GGTCAGGCATAGACCTGACCAAGGTGCCAGCTATTCAACAGAAAAGAACCGTGGCCTTTCTAAACCAGTT TGTGGTGCACACTGTGCAGTTTCTCAACCGGTTTTCCGCAGTTTGTGAGGAGAAACTGGCAGACCTTTCT CTCCGGATTCAGCAAATTGAAACAACTCTCAATATTTTAGATGCAAAGCTGTCATCTATCCCAGGCCTAG AGGACGTCACAGTTGAAGTGTCTCCTTTAAATGTCACTGCTGTCACAAACGGATCACATTCTGAAACCAC TTCGGAGCAAACGCAGCAGAATAGCACACAAGACTCTGGAGCACAGGAGAGTGAAGCGCCATCAGAAAAT GTCCTGACCGTAGCCAAGGATCCGCGGTATGCCAGATACCGCAAGATGGTTCAAGTGGGTGTTCCAGTGA TGGCAATCAGAGACAAGATGATCTCAGAAGGACTGGACCCGGAGCTACTGGAGAAGCCAGATGCCCCGGT GCCAAATGGCGAAAGCAAAAGAGCCGTAGAAGAAAGTTCAGATAGTGACTCTTCCTTCAGTGACTAAGGG ACTGGGGGAATCACACAGAACGTGGAAGAATGTATTTACGTTCTAGAAAAGAGCCTACGCTCTATCTAGC TCTCCGTGATAAACATCACCAAATGCTACTGCATACCTACCCCAACCTTTCTCTGTTTAAAAATAATTAT AATAATAAAGCATTTATAATCTTTCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3661472 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3661472 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11104 same embryo
	EMAGE:11101 same embryo
	EMAGE:11103 same embryo
	EMAGE:11100 same embryo
	EMAGE:11102 same embryo
	EurExpress:euxassay_003569 same experiment
	MGI:4823680 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source