EMAGE mouse gene expression spatial database

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EMAGE:11100

Mepce methylphosphate capping enzyme ( MGI:106477)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003570. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003570_01	euxassay_003570_02	euxassay_003570_03	euxassay_003570_04

euxassay_003570_05	euxassay_003570_06	euxassay_003570_07	euxassay_003570_08	euxassay_003570_09

euxassay_003570_10	euxassay_003570_11	euxassay_003570_12	euxassay_003570_13	euxassay_003570_14

euxassay_003570_15	euxassay_003570_16	euxassay_003570_17	euxassay_003570_18	euxassay_003570_19

euxassay_003570_20	euxassay_003570_21	euxassay_003570_22	euxassay_003570_23	euxassay_003570_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11100_wholemount_strong.wlz

11100_wholemount_moderate.wlz

11100_wholemount_weak.wlz

11100_wholemount_possible.wlz

11100_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11100_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6713
Entity Detected:	Mepce, methylphosphate capping enzyme ( MGI:106477)
Sequence:	sense strand is shown >T6713 CACTCGGGGAAGGAGGGAGTGAGGTGGTCTTAGGAAGGGTTACAGGGGGCTTCCCCTTGCTCTCTTCTCA GTACCCTCGCTTGAGAAGGGGAAATTAAACAGGGAGAGGAGATGATCGAGATGGCGGCGGAGAAGGAGCC CTTTCTGGTGCCGGCCCCGCCGCCGCCGCTCAAAGATGAGTCGGGCGGAGGGGGCGGCCCCGAGGTGCAA TCGCACCAAGAGGCTGCCTCCGGGGAGCTCCGTGATGGGACAGAACACGGGCCGGGCCCTCGCGCGCACT CGGCCGGGGCCGCAGCCAGCGGAGGGGGCGGCCCCCAGGCACAGGCGCACGGGGAGCCCCACGGGCGAGC CGCCGCTCCTGCGGACGTGGGGGAGGAGCGCCGGGGAGGGGGCGGGACGGACCTGGGTCCCCCAGCCCCT CCTCGACCTCGCAATGGCTACCAGCCCCACCGACCCCCTGGGGGTGGTGGGGGCAAAAGAAGAAATAGCT GTAACGTGGGGGGAGGGAGCGGTGGAAGCTTCAAACATCCGGCCTTCAAGAGGCGCCGGCGAGTGAATTC GGACTGCGACTCTGTGTTACCATCCAATTTCCTTCTGGGGGGTAACATATTTGATCCACTGAACCTGAAT AGCCTCCTGGATGAGGAAGTGAGTCGCGCACTCAATGCGGAGACCCCAAAGTCATCCCCACTTCCGGCCA AGGGGCGAGACCCTGTGGAGATCCTCATCCCCAAAGATATCACTGACCCGCTCAGTCTCAACACTTGCAC TGACGAGGCCCATGTAGTGCTTGCCTCACCACTCAAGATTGGTCGCAAGCGCCATAGACACCGGGGACCG CACCACCAGCAGCAGCAGGCGTCTGGAGGGAATGATAGCAACGCCGCCGTGCTGCCCACTGACCCTCTCA CGCCCTCACTCCATGGGGAGGGTGCTACGCAGCAGCAGCAAAACAGAGGCCAGAACCGTGATGCCCCTCA ACCCTATGAACTCAACACAGCCATCAACTGCAGGGATGAAGTTGTGTCTCCCCTTCCATCTGCCCTGCAG GGTTCCTCAGGCTCCCTTTCTGCCCCTCCAGCTGCCTCAGTTACCTCTGCACCCTCTACTTCTTCCTCCT CCCGACATCGCAAACGTCGCAGGACTTCCAGCAAGTCAGAGGCAGGGGCTAGGGGTGGAAGCCAGGGTTC CAAGGAAAAGGGCAGAGGGAGTGGAGGAGGCCGTCACCACCACCACCCACTACCTGCAACTGGCTTCAAA AAGCAACAGCTAAAGTTCCAATATGGGAATTACTGCAAGTACTATGGCTATCGCAATCCTTCCTGTGAGG ATGTGCGCCTTAGGGTGTTGAAGCCCGAGTGGTTTCAAGGCCGGGACGTCCTAGATCTGGGCTGCAACGT TGGCCATCTGACCCTAAGTATTGCCTGCAAGTGGGGCCCAGCCCGCATGGTGGGGCTGGACATTGATCCA CGGCTTATCCACTCTGCTCGCCAAAATATCCGACACTACCTGTCGGAGGAGCTTCGTCTCCAAGCTCAGA CTTCTGAGGGGGACCCAGGGACAGAGGGGGAGGAAGGGACTATCACTGTCCGAAAAAGGAGCTGCTTCCC AGCCTCACTGACAGCCAGCCGGGGTCCCATCGCTGCACCCCAAGTGCCCTTGGATGGAGCGGACACATCT GTCTTCCCCAACAATGTCGTCTTCGTCACGGGTAACTATGTCCTGGACCGAGATGAGCTGGTGGATGCCC AAAGACCGGAGTATGATGTGGTGCTGTGCTTCAGCCTCACCAAGTGGGTGCACCTGAACTGGGGTGATGA GGGGCTGAAGAGAATGTTCCGCCGCATCTACCGGCACCTGCGCCCTGGGGGTATTCTGGTCCTGGAGCCC CAACCATGGTCCTCCTACTGCAAGAGAAAGTCTCTCACGGAAACAATCTATAAGAACTACTTTCGAATCC AGTTGAAGCCAGAACAGTTCAGTTCCTACCTGACATCCCCAGAGGTGGGTTTCTCCAGCTATGAGCTTGT GGCCACACCGAATAACACCTCCAGAGGCTTCCAGCGTCCTGTGTACTTGTTCCACAAGGCTCGATCCCCC AGCCACTAAGTGGCCTTCTGAGAAGTTGTAAAGAGGCTGCCCTTGCTGCTCCTGAGGACCTGGGGGGAAA GGAAATTATTCCAAGGTCTTTCCTAACTCCAAAAAGTTTCTCCTTTTCTTGGATCTGTCAAGAGAGCTTT TCCTAAGTTGCTACCCCACATCTCCTCCCTGTCACCTCTGGCACTTGAGCAAGCCCAGCTGTGCTGGAAT TCCATCAGCTTCCTCTCCTAGCCCGCTGGGCTGGATGGCATGGACTGTTTGCTGACTCTGCCCACCTGGG ACAGGGGAAGGGGGAATGTCGAGTTCTCCAGGCCCTTCCTCCTGTTCTCAGGGGACTTCCCGCTTCTCCT CAGCCTTTGTTCCTAGCTGCACTTAAATGGACCATGGATGGACTAAGGGTGTTAGAGGGGAAGTTTGGTA AGGAGACACACAGGTACTGTGAAAGTCCTTTTGCTGTCCCCCGGTGGGAGGGAGCTGGGTTTGGAAATGT GAGAACAGCACAATAAACTTGATAACTAGGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3662118 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3662118 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11104 same embryo
	EMAGE:11101 same embryo
	EMAGE:11103 same embryo
	EMAGE:11099 same embryo
	EMAGE:11102 same embryo
	EurExpress:euxassay_003570 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source