EMAGE mouse gene expression spatial database

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EMAGE:11165

Rassf6 Ras association (RalGDS/AF-6) domain family member 6 ( MGI:1920496)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003495. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003495_01	euxassay_003495_02	euxassay_003495_03	euxassay_003495_04

euxassay_003495_05	euxassay_003495_06	euxassay_003495_07	euxassay_003495_08	euxassay_003495_09

euxassay_003495_10	euxassay_003495_11	euxassay_003495_12	euxassay_003495_13	euxassay_003495_14

euxassay_003495_15	euxassay_003495_16	euxassay_003495_17	euxassay_003495_18	euxassay_003495_19

euxassay_003495_20	euxassay_003495_21	euxassay_003495_22	euxassay_003495_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11165_wholemount_strong.wlz

11165_wholemount_moderate.wlz

11165_wholemount_weak.wlz

11165_wholemount_possible.wlz

11165_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11165_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14

vibrissa

moderate

regional

moderate expression: see section 05 06 07 08 20 21 22

stomach

moderate

regional

moderate expression: see section 12 weak expression: see section 04 05 06 07 08 09 10 11

hindgut

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 10

rectum

moderate

regional

moderate expression: see section 13 14

midgut

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 weak expression: see section 06 07 08 09 10 11 19

liver left lobe

moderate

regional

moderate expression: see section 01 02 weak expression: see section 03 04 05 06 07 08 09 10 not examined expression: see section 14 15

liver right lobe

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18 19 20 21 22 23

not examined

regional

not examined expression: see section 19

bladder

moderate

regional

Expression in the fundus region.

kidney calyx

moderate

regional

moderate expression: see section 08 09 10 19 20 21

urethra of male

moderate

regional

moderate expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6730
Entity Detected:	Rassf6, Ras association (RalGDS/AF-6) domain family member 6 ( MGI:1920496)
Sequence:	sense strand is shown >T6730 CCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGCTTTGCCCGGCACAAAAGCAGGTGAACTTGGAGGT TGAAGCGAAGAGCCCCGCTCAGTCCACACACAGGCACACCAGGGCACCCTCAGATCATCTTGGGAAGGGA GAAAGGATGACAGCAATGGATCACCAGTTCCCTTCTTGGATCGTTGTCAATGAGAGCACATCCATAAGCA GGGAACAACTCAATTATTTACTGGAGACTTATAATGTTTTCTATGAAAACCAGAAAAATCTGCATATACT GTATGGGCAGACCGAAGACGGACAGCTGATTGTTGAAGGGATGCTGGACATTTTCTGGGGAGTCAAACGA CCCATACAGCTGAAAATACAAGATGAGAAGCAAATCTCTTCCTTTGATCTGTTGAACACACCGGAAACCT TTTCCAGCAAAGGGAGGATGACTCGCTGGGGAGAATTTGATGACCTTTACCGTATCAGCGAGCTGGACAG GACCCATGTTCTAGCATCTGAAGCCAGGCATTCCCCGGAAGACTATTTATCTTACCACAGCACTCTGACA CCATACGCAGACGAAGAGCCGGAATCCCCATTGCTCTACAGGACCATGAGTGAGGCAGAATCCCCATTGC TCTACAGGACCATGAGTGAGGCAGCTTTGGTGAGAAAAAGGATGAGGGCCCCAGAGATGTACAGAAAAGA TAGAATGGGGGTGCTAAGTAACCACAGAGCCTCTATCAACGGACACGTCTATGACCATGAGACATCCATC TTCACCCCAACTTTCGGATCAGAGACTAAGGTCAGAGCCAATAGTATCATGAGAACTGAAGAAGTAATAA AGCAACTTCTCCAGAAATTTAAGATTGAGAATAGCCCTCGGGATTTCGCTCTTTACATTATTTTTGGGAC AGGAGAGCAGAGAAAGCTAAAGAAGACCGATGTCCCACTGCTGCAGAGGTTACTACAAGGACCATCCAAA AGCAATGCTCGGATCTTCCTCATGGATAAAGATGCAGAAGAAATCAGCAGAGATGTGGCTCCGTACATTA ATTTTCACTTTTCTTTCTTGGAATCCATCCTTCAAAGATTAGATGAAGAAGAGAAAATGGAGATTGAGAG AATTATGGCAAAATTCAATACCGAAAGGGCCTTTATCCTGAAGTGTCTACAGAGTAAACAGGCAGCTAAA ACGGAGACCACAGTCTAGAAGCCCTGCTCAGCGGAGTTAGAGGGAGCACTGCAGAATCCGTGTGTGGTCT GCTCTGCATCCTAATATGCATGGCTCTGAAGCTTTGGACGAACGAATGCCGAGCAGCCTGCTTCCTCTTT GAAATGCAGAGGTTGGCTGCGGTTCAGAGTTGGACTAAGGCAAGATGGGACTTCATTTCAGTAACTGAAG GGAAGCCTGCACACTGCCCATTCCAAATGTCCTCGATAGCAAGTTCTCGGGTGAGCTCAGACTCAAGTGG ACTCTGCTCTTATCACCTGGTTTAAGCTTGTTTTATCTTGCGCTCTTAAAAGAGCCGAGATACTTGATGA CTCATAAATGACGGGAAGTAAACATTTCCAGTTCCTGATGTCAACAAGAAACACATTCTCAAGCCATCAG CGATCTGAACTGACCCTGTTTCACAGCTGTGTAAACATCTGAGCATTTTCATAAGATTTAGGAATAGATC TACAAGGGCTGATGATTTTAAACTCTGAAGTATCAGAGTACCCGACCTTTTAAAATAAAGTGGTTTTGGA TTTTTGTAAAGAAACCTAAACTCGAGGCTGGAGTGATGGCTACGTGGTTAAGAGCATTGACTACGCTTCC AGAAATCCCCAGTTCAGTTCCCAGCATCTACAGGGCAACTGGCAATCATCTGCAACCCCCGTCCTAGAGG ATCTGGTACCCACCTATGTGGGCATTGCAAGCACGGGGCACACAGACACAGAGGGAGGCAAAACAGCTAT ATATGTTATAAATAAACAAATATTATTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3669766 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3669766 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11169 same embryo
	EMAGE:11166 same embryo
	EMAGE:11164 same embryo
	EMAGE:11168 same embryo
	EMAGE:11167 same embryo
	EurExpress:euxassay_003495 same experiment
	MGI:4827637 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source