EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11214

Gnaz guanine nucleotide binding protein, alpha z subunit ( MGI:95780)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001214. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001214_01	euxassay_001214_02	euxassay_001214_03	euxassay_001214_04

euxassay_001214_05	euxassay_001214_06	euxassay_001214_07	euxassay_001214_08	euxassay_001214_09

euxassay_001214_10	euxassay_001214_11	euxassay_001214_12	euxassay_001214_13	euxassay_001214_14

euxassay_001214_15	euxassay_001214_16	euxassay_001214_17	euxassay_001214_18	euxassay_001214_19

euxassay_001214_20	euxassay_001214_21	euxassay_001214_22	euxassay_001214_23	euxassay_001214_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11214_wholemount_strong.wlz

11214_wholemount_moderate.wlz

11214_wholemount_weak.wlz

11214_wholemount_possible.wlz

11214_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11214_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

weak

regional

weak expression: see section 05 06 07 08 20 22 23 24

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 19 20

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 10 19 20 21 22 23 24

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 08 19 20

thoracic ganglion

weak

regional

weak expression: see section 16

dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4217
Entity Detected:	Gnaz, guanine nucleotide binding protein, alpha z subunit ( MGI:95780)
Sequence:	sense strand is shown >T4217 CTTAGAAACATCGCCACAGCAACCAGCGAGCAGACAGCAGTAGCCTGGGCAGACGCAAGCGGACAGCTTC CTACCGTGGCAGAGTACAGGGAATGACTACGGCAAATCAGGCCACACTGCTGACAAGGGAGGTGGAGTGT CACTAGAGGGGAGGGTGTGGTCTCTGCCCCACTGCACCAAGCGCCATGCCCACAGGAGAAGCGGTACTGG GGCAGGGATTGCTCTGTGACACAGCCTCGCCCCAAAGCCAGTGCTGAGCACGGCCGGGTCAGCTGCCTCT CTCATCTGCCCGTCACACCAGCCCACGTTTGAGCATCCCTCGTTGTGACCATTCTGTTTGGCGAGGGGGA GAGGCGCCCACCCTGTGTTCTGCATCTGGGGGGTGGCCCGCTGCTGCCGGACCATGGGATGTCGGCAAAG CTCAGAGGAAAAAGAGGCAGCGAGGCGGTCCCGGAGAATTGACCGCCACCTGCGCTCCGAAAGCCAGCGG CAGCGCCGTGAAATCAAACTTCTCCTGCTGGGCACCAGCAACTCGGGCAAGAGCACCATCGTCAAGCAGA TGAAGATCATCCACAGCGGGGGCTTCAACCTGGACGCCTGCAAGGAGTACAAGCCCCTCATCATCTACAA CGCCATCGACTCGCTGACCCGGATCATCCGGGCCCTGGCTGCCCTCAAGATCGATTTCCACAACCCTGAC CGTGCCTACGACGCTGTGCAGCTCTTTGCTCTGACTGGCCCAGCAGAGAGCAAGGGTGAGATTACACCTG AGCTGCTGGGTGTCATGCGACGGCTCTGGGCTGACCCAGGGGCCCAGGCCTGCTTTGGCCGCTCTAGCGA GTACCACCTGGAGGACAATGCAGCCTACTACCTGAACGACCTGGAGCGCATCGCAGCGCCCGACTACATC CCCACGGTGGAGGATATCCTACGCTCCCGGGACATGACCACGGGCATTGTGGAGAACAAGTTCACCTTCA AGGAGCTTACCTTCAAGATGGTGGACGTGGGCGGGCAGAGGTCAGAACGCAAAAAGTGGATCCATTGCTT TGAAGGCGTCACAGCCATCATCTTCTGTGTGGAGCTCAGTGGCTATGACCTGAAGCTCTATGAGGACAAC CAGACGAGCCGGATGGCGGAGAGCCTGCGCCTCTTTGACTCCATCTGCAACAACAACTGGTTCATCAACA CCTCGCTCATCCTCTTCCTGAACAAGAAGGACCTCCTGGCAGAGAAGATCCGGCGTATCCCGCTCAGCGT CTGCTTCCCAGAGTACAAGGGTCAGAACACGTACGAGGAAGCCGCGGTCTACATCCAACGTCAGTTCGAG GACCTCAACCGCAACAAGGAGACCAAGGAGATCTATTCGCACTTCACTTGTGCCACCGACACCAGTAACA TCCAGTTTGTGTTTGACGCAGTGACAGATGTCATCATACAGAACAATCTCAAGTACATCGGCCTTTGCTG AGGAGCCGGGCGCAGCCTGCTCGCCTGTGGTGAAACCCATGGGGTGTCACACCCCACACCTCATGCTCGA GAGGCCCGACCCAGGGGCAGAAAACGGGGCTTGAAGAGTGTGTCCCCATCCCACCCCCAGCCTCTGCCTT CTTGGCCCCACATCTCTGCAAACATAAATATATTTGGATAGATTGCTAGGTAGGTAGACACACAGACACA CACACAGACACACACACACACAGACACACGCACGCACGCACATCTGGAGAGGGCATAGTTCTCCAGAGCG TCAAGGTTTCCTGAAGTTTTCAAAAGCTGCTGTCACAATTTCATTCCGAGGCCATCTTGCCCCTCCCCCT TCACTCTGCCTTCCTGAGTTGGCCCCACTCTGCACGGGAGGGAGGGCCCACCTTAGACTGCTGGGGAGAG GCCACAGGGCTGGGGGCCAGGTCCGGCCAGCTGTGCCCATCGCCTGGGAGAAGGCCAGCTCACCAACTGA GCTCTGGACTGGGGACTACCGCTGACCAGGAGGGAAGCCTGGAGCCGCACCCGACTCAGCCACTCTGCGT CACATTCGTCCTGTACCTAGGACCGGAGGAAAGACGTGACTGCTCTAACCGGACCTCTGACTACCGGTGG CTTTTACAAAAAAAAAACAGCTAAAACGTACTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4501587 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4501587 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11211 same embryo
	EMAGE:11213 same embryo
	EMAGE:11212 same embryo
	EMAGE:11210 same embryo
	EurExpress:euxassay_001214 same experiment
	MGI:4825131 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source