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EMAGE:11219

Med6 mediator of RNA polymerase II transcription, subunit 6 homolog (yeast) ( MGI:1917042)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11219 EMAGE:11219 EMAGE:11219 EMAGE:11219 EMAGE:11219
"Pseudo-wholemount" of euxassay_003411. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003411_01 euxassay_003411_02 euxassay_003411_03 euxassay_003411_04
EMAGE:11219 EMAGE:11219 EMAGE:11219 EMAGE:11219 EMAGE:11219
euxassay_003411_05 euxassay_003411_06 euxassay_003411_07 euxassay_003411_08 euxassay_003411_09
EMAGE:11219 EMAGE:11219 EMAGE:11219 EMAGE:11219 EMAGE:11219
euxassay_003411_10 euxassay_003411_11 euxassay_003411_12 euxassay_003411_13 euxassay_003411_14
EMAGE:11219 EMAGE:11219 EMAGE:11219 EMAGE:11219 EMAGE:11219
euxassay_003411_15 euxassay_003411_16 euxassay_003411_17 euxassay_003411_18 euxassay_003411_19
EMAGE:11219 EMAGE:11219 EMAGE:11219 EMAGE:11219 EMAGE:11219
euxassay_003411_20 euxassay_003411_21 euxassay_003411_22 euxassay_003411_23 euxassay_003411_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11219Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11219_wholemount_strong.wlz
11219_wholemount_moderate.wlz
11219_wholemount_weak.wlz
11219_wholemount_possible.wlz
11219_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11219_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 11 13 15 16 weak expression: see section 12
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 16 17 18 19
pancreas
moderate moderate
regionalmoderate expression: see section 08 09 10 11
brain
moderate moderate
regionalmoderate expression: see section 23
vibrissa
moderate moderate
regionalmoderate expression: see section 04 05 06 07 18 19 20 22
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 01 02 03 22 23 24
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 18 weak expression: see section 15 16 17
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 10 11
tongue
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 weak expression: see section 14
midgut
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 10 11 14 15
lower jaw molar
moderate moderate
regionalmoderate expression: see section 05 06 17 18 19 weak expression: see section 07
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 10 11 14 15
upper jaw molar
moderate moderate
regionalmoderate expression: see section 05 06 17 18 19 weak expression: see section 07
liver
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
metanephros
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 17 18 19 20 21
lung
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6818
Entity Detected:Med6, mediator of RNA polymerase II transcription, subunit 6 homolog (yeast) ( MGI:1917042)
Sequence:sense strand is shown

>T6818
GCGGCGGTGGATATCCGAGATAACCTGCTGGGGATCTCCTGGGTTGACAGCTCTTGGATCCCCATCTTGA
ACAGTGGCAGTGTCCTGGATTACTTTTCAGAAAGAAGCAATCCTTTCTATGACAGGACGTGTAATAATGA
GGTGGTCAAAATGCAGAGGCTGACATTAGAGCACTTGAATCAGATGGTTGGAATCGAATACATCCTTTTA
CATGCTCAAGAGCCCATTCTTTTCATCATTCGAAAGCAACAGCGGCAGTCCCCTGCTCAAGTTATTCCAC
TGGCTGATTACTATATCATCGCTGGAGTCATCTATCAGGCGCCAGACTTGGGATCAGTTATAAACTCCAG
AGTGCTTACTGCAGTGCATGGAATTCAGTCAGCTTTTGATGAAGCTATGTCATACTGTCGATACCATCCT
TCCAAAGGATATTGGTGGCACTTTAAAGATCACGAAGAACAAGAGAAAGTCAAACCTAAAGCCAAAAGGA
AAGAAGAACCAAGCTCTATTTTTCAAAGACAGCGTGTGGATGCTTTACTCATAGACCTTAGACAGAAATT
TCCACCCAGATTTGTTCAGCAAAAGTCTGGAGAAAAGCCTGTCCCAGTGGATCAGGCAAAAAAAGAGGCA
GAGCCTTTGCCAGAAACTGTAAAATCTGAGGAGAAGGAGAGCACAAAGAACATTCAGCAGACTGTGAGCA
CTAAAGGTCCCCCTGAGAAACGAATGAGACTCCAGTGAATGTTGGACGAGGAAGAAGCTGGCCAGCTCCT
TGTCAGTCGCTATACCTCAGCACCGTGGGAGGCTCTTGAGCCTGGAAACACTATCGCAGTTTGTACGGAA
TGCACTTGTTCTTTACAAAAGGACGTTCTCCCTACCATGTTTTCTATGTGAATATTTCTTAAATATCTTT
TGTAATCTTTCCTCTACTGGACTTGGGCAGTGCACCTAACTCACAGATATGCCAGTATATATCTTTAAAA
CAATAAAGTTATTTGTATATATTAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3710612 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3710612 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11223 same embryo
 EMAGE:11220 same embryo
 EMAGE:11222 same embryo
 EMAGE:11221 same embryo
 EurExpress:euxassay_003411 same experiment
 MGI:4824408 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS