EMAGE mouse gene expression spatial database

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EMAGE:11221

Amfr autocrine motility factor receptor ( MGI:1345634)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003433. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003433_01	euxassay_003433_02	euxassay_003433_03	euxassay_003433_04

euxassay_003433_05	euxassay_003433_06	euxassay_003433_07	euxassay_003433_08	euxassay_003433_09

euxassay_003433_10	euxassay_003433_11	euxassay_003433_12	euxassay_003433_13	euxassay_003433_14

euxassay_003433_15	euxassay_003433_16	euxassay_003433_17	euxassay_003433_18	euxassay_003433_19

euxassay_003433_20	euxassay_003433_21	euxassay_003433_22	euxassay_003433_23	euxassay_003433_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11221_wholemount_strong.wlz

11221_wholemount_moderate.wlz

11221_wholemount_weak.wlz

11221_wholemount_possible.wlz

11221_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11221_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6813
Entity Detected:	Amfr, autocrine motility factor receptor ( MGI:1345634)
Sequence:	sense strand is shown >T6813 GCAGCGGCAGCGACGCCAGAGCCCGTGGGCGCCTTTCGCAAGGCCGCCGCAGGCGCCTGGCCGCAGCGCG GGAAGCCTCGGGGGCCGGAGCCCGTCGCTGCCGCCATGCCGCTGCTCTTCCTCGAGCGCTTCCCGTGGCC CAGCCTCCGCACCTACACGGGCCTCAGCGGCCTGGCCTTGCTGGGCACCATCGTCAGCGCCTACCGCGCC CTCAGTCAGCCGGAGGACGGCTCGGGTGAGCCGGAGCCGCTAACGGCCCCACTGCAGCCCGAGGCGCGCC TGACCGCCGGCGGCCCCCGGGCCCGCGACGTGGCCCAGTACCTGCTGTCGGACAGCCTGTTCGTGTGGGT TCTGGTGAATACTGCTTGCTGTGTTTTGATGTTGGTGGCTAAGCTTATCCAGTGTATTGTGTTCGGCCCT CTTCGAGTGAGTGAAAGACAGCATCTCAAAGACAAGTTTTGGAATTTTATTTTCTACAAGTTCATTTTCA TTTTTGGAGTGCTGAATGTCCAGACTGTGGAGGAAGTGGTGATGTGGTGCCTCTGGTTTGCTGGACTTGT CTTCCTGCATCTGATGGTTCAGCTCTGCAAAGACAGATTTGAATACCTTTCCTTTTCTCCCACCACTCCA ATGAGCAGCCATGGGAGAGTCCTGTCCCTGCTGATTGCTATGCTGCTCTCCTGCTGTGGACTGGCAGTCG TCTGCTGTGTCACAGGCTACACACATGGCATGCACACCTTGGCTTTCATGGCTGCAGAGTCTCTTCTTGT GACCGTGAGAACAGCTCATGTGATTTTAAGATATGTAATTCACCTCTGGGACCTCAACCACGAAGGGACG TGGGAAGGAAAGGGAACCTATGTGTATTACACCGACTTTGTCATGGAGCTCGCTCTCCTGTCCCTGGACC TCATGCACCACATCCACATGCTGTTATTTGGCAACATCTGGTTATCTATGGCTAGCTTGGTCATCTTTAT GCAACTTCGTTACCTGTTCCATGAGGTACAGCGTCGAATCCGTCGACACAAGAATTATTTGCGAGTGGTA GGAAACATGGAAGCCAGGTTTGCTGTTGCAACTCCAGAGGAACTAGCTGTCAATAATGATGACTGTGCAA TCTGCTGGGACTCCATGCAGGCTGCAAGGAAACTGCCCTGTGGACATCTTTTTCACAACTCCTGCCTTCG TTCTTGGCTAGAACAAGACACCTCTTGTCCAACATGCAGAATGTCTCTTAATATTGCTGATGGCAGTCGT GCCAGGGAAGACCATCAAGGAGAGAACCTGGACGAGAACTTGGTTCCTGTAGCAGCTGCCGAAGGCAGGC CTCGCTTGAACCAGCACAATCACTTCTTCCACTTCGATGGGTCCCGGATTGCAAGCTGGCTGCCAAGTTT TTCAGTTGAAGTGATGCACACTACCAACATTCTTGGCATCACGCAGGCAAGCAACTCTCAGCTAAATGCC ATGGCTCATCAGATTCAGGAGATGTTCCCTCAGGTCCCGTACCACCTTGTGCTCCAGGACCTCCAGATGA CACGCTCCGTGGAGATAACAACAGATAACATCTTAGAAGGACGGATTCAAGTACCTTTCCCCACGCAGCG GTCGGATAGCCTGAGGCCTGCACTGAACAGCCCAGTGGAGAGGCCCAGCCCTGACCTGGAGGAGGGAGAA GCTTCTGTCCAGACGGAGCGTGTACCACTGGACCTCAGTCCTCGCCTAGAGGAGACCTTGGACTTCAGTG AGGTAGAACTAGAGCCTATTGAGGTGGAAGACTTTGAGGCCCGTGGAAGCCGCTTCTCCAAGTCTGCTGA TGAGAGACAGCGCATGTTAGTTCAGCGCAAGGATGACCTCCTGCAGCAGGCTCGAAAGCGGTTCTTAAAC AAAAGTTCTGAAGATGATGGGGCCTCAGAGAGACTCCTCCCTTCTGAAGGCACATCCTCTGACCCCGTGA CCCTGCGCCGAAGGATGCTGGCAGCCGCTGCTGAGCGGAGACTTCAGAGGCAACGGACAACCTAGTGCTT CCTCACCTTCCTCAGCCTCCTGCCCAGCTCTACCTACCGGAAGAGGCCTGTCCCAGTTCTGCCTGCTGTG TGGAGACCTGCGACTGCATTGCTGCTGCATAAAGCATGTGGTCTTTAGTGTGTGGACAGCTGACAAATTT AATCCTTTGTAATACTTTCTTTGTGACATTTCTCTTCCCCTTAGAAACATTGCACATTTTAACTCTAGGC ATGGCCTCTTCTGGTGTTGACTGGACTTGTTGGGAGTAGGGGACGAGCAAAAAGAAGTGGGCACTGGGGC GCTCGCCTGCCTGCAGCAGCTAGTTTGAATTTCTGTTTTGATATAAACCACAGCAGATGTTGAGTATTTT AGCACCACAGCTGGAAGGAAGCACGGATCTTGGGGAGGGTGCATAACAGCAGTCAGAAGGTGCGCAGCAG GAGCCTGACCACCACTATGGCAAAGTAGCCTACGATTAAATGCTGTGCTTAGGGAGGAGGCCAGTCCCAC AGGCGCCTGGACCTCTAGACCAGCAGGACATGGTGTAGAAAGAACTGGTTTGGTACCTGGAGTAGAAAGA GGAAGCTCAGGGCTGCAGAATGAGGAGGCCCTTTTCTGCCCTTAACATTCAGGACCAATCTGAGCCCAGT CCCCAGCTGGCTTCACTTCCGTCACTCTGGTGCTCTCGCATCACAGAAGCTTGCCCCCATGGCTGCCGCC TCTTCCTTTCCTTTGGTTATGACTTTAATTCCTTGGTCAACCCAATTAGGCTATGCTAAACATATGGGTT TGATCCTTGGAAAAAGGCATGGGGCCTGCCCAGCAAGGGCAGGGGTGTTTGGAGGACACTGCTGAAGAAA CACCTTTCGTATCTTTGTCCCTGGAGCCCTTGAGTTCCCTGCTTTTGTTCCTGTGGGTCTCATTAAACGG AAACAGAGGATGTTGAATAATCCACATGCCTCAAGCAGACTCGGTCCCAGATCGGCTCAAATTCTCCACG TATTTAAGGATTTGAAACATGCAATCATAGGATACATAGTTTTATGTCCCATTGGATTAAGGTTTTTTTT TTTTAATGTTTCAAAACATAGTTTTATGGTAGTCCTTTTGGGAAAAATCCAGTATTATCTAAAATTATTG GCAAAGTTAAATGTATTTTACATACCTGGAAGTTTTTAGACTGTTGATAAGTAACTGAGAAAGGATAACA GGCGCCTAGGTGAAGATAATTTATTTCAATAAATCTTTCAAAAGCCTTACCTTGAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3710780 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3710780 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11223 same embryo
	EMAGE:11220 same embryo
	EMAGE:11219 same embryo
	EMAGE:11222 same embryo
	EurExpress:euxassay_003433 same experiment
	MGI:4823108 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source