EMAGE mouse gene expression spatial database

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EMAGE:11229

Cnr2 cannabinoid receptor 2 (macrophage) ( MGI:104650)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003189. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003189_01	euxassay_003189_02	euxassay_003189_03	euxassay_003189_04

euxassay_003189_05	euxassay_003189_06	euxassay_003189_07	euxassay_003189_08	euxassay_003189_09

euxassay_003189_10	euxassay_003189_11	euxassay_003189_12	euxassay_003189_13	euxassay_003189_14

euxassay_003189_15	euxassay_003189_16	euxassay_003189_17	euxassay_003189_18	euxassay_003189_19

euxassay_003189_20	euxassay_003189_21	euxassay_003189_22	euxassay_003189_23	euxassay_003189_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11229_wholemount_strong.wlz

11229_wholemount_moderate.wlz

11229_wholemount_weak.wlz

11229_wholemount_possible.wlz

11229_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11229_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4879
Entity Detected:	Cnr2, cannabinoid receptor 2 (macrophage) ( MGI:104650)
Sequence:	sense strand is shown >T4879 GGCCAAGCTGTGCCTGAATGAGCAGAGGCACAGGCACCAGCCGTGGCCACCCAGCAAACATCTCTGCTGA CTCAGACTGGGCCCAGTCTTCTGGGACAGCTCCAGTAGAAGAAGCCAAAGCCCATCCATGGAGGGATGCC GGGAGACAGAAGTGACCAACGGCTCCAACGGTGGCTTGGAGTTCAACCCCATGAAGGAGTACATGATCCT GAGCAGTGGCCAGCAGATCGCCGTGGCGGTGCTGTGCACCCTGATGGGGCTGCTGAGCGCCCTGGAGAAC ATGGCCGTGCTCTATATTATCCTGTCCTCCCGGCGGCTCCGCAGAAAGCCCTCGTACCTGTTCATCAGCA GCTTGGCTGGAGCTGACTTCCTGGCCAGCGTGATCTTCGCCTGCAACTTTGTCATCTTCCACGTCTTCCA CGGGGTCGACTCCAACGCTATCTTCCTGCTGAAGATCGGCAGTGTGACCATGACCTTCACAGCCTCTGTG GGCAGCCTGCTGCTAACCGCTGTTGACCGCTACCTATGTCTGTGTTACCCGCCTACCTACAAAGCTCTAG TCACCCGTGGGAGGGCACTGGTGGCCCTCTGTGTCATGTGGGTCCTCTCAGCATTGATTTCTTACCTGCC GCTCATGGGGTGGACTTGTTGCCCTAGTCCCTGCTCTGAGCTTTTCCCACTGATCCCTAACGACTACCTA CTGGGCTGGCTTCTATTCATTGCCATCCTCTTTTCCGGCATCATCTATACCTATGGGTATGTCCTCTGGA AAGCCCACCGGCATGTAGCCACCTTGGCTGAGCACCAGGACAGGCAGGTGCCTGGGATAGCTCGGATGCG GCTAGACGTGAGGTTGGCCAAGACTCTGGGCCTGGTGCTGGCTGTGCTGCTCATATGCTGGTTCCCTGCA CTGGCTCTCATGGGCCACAGCCTGGTCACCACGCTGAGTGACCAGGTCAAGGAGGCCTTCGCCTTCTGTT CCATGCTGTGCCTTGTTAACTCTATGGTCAATCCTATCATTTACGCCCTGCGGAGTGGAGAGATTCGCTC TGCTGCCCAGCACTGCCTGATAGGCTGGAAGAAGTATCTACAGGGCCTCGGACCTGAGGGGAAAGAAGAA GGCCCAAGGTCCTCGGTTACAGAAACAGAGGCTGATGTGAAAACCACCTAGGAGCCAGGATCCAGAACTC CAGGCTGCTCCAACTGCTGACACCACCTGTCTTTCTACTGGAAACAGCCCGAGTCAGAAGTCCGTTCAGT TCACTCCCTCGAAGAGAGAGGGGTTCCAGACCCGGATTCTTTCTGAAGCCAGTTCTAGGGTAATGGAAAC AGATCCCCCTTTTTGCTAAGGAATGCTGGTTGGCTGGAAGGTGGCCTGGTCATGCCTACCTTTTCCAGTC TGTGGAGAGGGGCCTGTAAGAATGGCAGGCTCTGTTCATATCAAATCAGGAAAGCCTTCTGACTCCAAAG ACCTAGAGGCCAGAGGGGCCTCCAGAGATGATACTAAAGGATTCAGGAGATCTGTGAAGACAAGGGACCT GTTCTCCTGGAATCTAGAGTACCCCAGCCGTGGGGCCACTGCCCTCAACTTCATAGATGTCCATGTCTTC CATAGTGTGGATTCTAGACTGTCCTGCTGAAGATAGAGAGACCAGCTGCTTCTGGTTCCTGCTTCATCCT CTGTGGCTGTAGAGCTATGTGAGGCTCTACCTTCAGGTCACCCTTGCCTCTTGGGGGTCAAGAATTATGA TGCCCTAAGGACCAAGGATGTTCGCTTACATCCTTCAGACAGCGTCAGGAGCTGTCAGCTCAGGGTATCC TGTTGTTGGGAAAGGGCCCTCCCGACATCCTGATAAGCCTTGAAAGCCATTATGACCTTAGGTCCTTAGG GGCGCAGAGGAAGCTGGGTTCACTGGAGGTACAAAGTCTTCAGAGAACTCTATTCCTTGTGACATTTTGC TGTGTCTCACACAGCACTATCCCAGTCTTGCCTAGCTTCAGTACAAAGCAAGATATTCACACAGATTCTT TGGGTCCTAGGCTGTTCCCAGTGATGCTTGACTCAGCCCCACTGGCCTCTTCCACTCTGGTCCTGAAGGA TAAGCAGAAGGAGTTGGGAGGGAAGACTTCCTGCTGCAAACCAGGCATCCTAGGTCCACAGCCCCACGCT GTGTCTCTGGGTGCCTGCCTTTGCCCTTCAGTAAAGGTTGCAGTCCCAACAGATGGAGGTTCAAGGCGCT GCTGGTGGTAGGAGGGGTAGGTGGCACTGGGGACCACAGACACAGTGAAAGGAAAACTCTTAAACATTTA ATACTTTTGCTTTTAGAAGATCCGGCCCGCAGACAGGACTTCCCCATTGCCCAAAGGAACTAACAGGGTG GTGTCCAGTGCAAAGAAAAATCAGCCTCCCTAATAAAGCAAGGAGGTCCACTCGGCTCTACAGCCTCTCC TAAGCTTCTGCAAGGTGACAGTTTGTTTTCTCTGTGAAGGTCAACGGCGTCTCATTTTATTCTTCCCAAG ACAAGTTCTGTGCATCAAAAGCTGCTGCAAATTCTAATCGCTGTTTGGGAGGATCAGGGGAAGAAGTCGA TTCGCCCACGCTTAGTGATTTAGACTGTATTTCAACATCAACTTGGGGGAGGAAGAGACAGAAAAGACAC GGTGTTTCCTTATTACTCCTCAAAGGAGGAAGTGCTTGGTTCTGTCAACATAAGCCGATCTCTCCAAGGT GCCTGACAGAGGAGGAGGGAATACAGTTTCCACAGCTGTGAATTCCTGCACCAGCTTTGGTCCACACTTA TGTGGTCTTGATCAACCCCCGCCCTTTACCTTGCTTTGGTTTCCTTCTCGGTAAATTGGGAATGGTGGGG AAAACAATGCTCTCCAGGGCCATTAAAACCTCTCATTGCTCTAACATGCTGTGGTTATCTCTGCAACCAT GGAGACCTTTGTGCCATATGGACCATGTGACTTCTGGCTCCCATACTTCCTGAAGTTATGGGCACACACC ATATACCATTGGGCGTGTGCTGACTGTAGAGGAATTGGGTGACTGGCCATATGAGTTTTAGAGATTTTCC CAGGGCAAGAGGGTTCTCTTGGCTCTCAATTTTTCTGGTCCCTCATGCCTCAGTGGTCCCCTGTGTGTGG GTCAGGCATGCTTGTTTAGCGGTGCCAGACTGAAGCTCCGGCCTTCCGGTGTACCAAGCAAGTGTTGTAC TCCTGAGTCAGGTTTTCTTCAGTTAGATGCTAACTAAGCTCTCTGAGAGACCCTCAAAGTTTTTGAGGTG TAGCTCAGTCAGTAGAGTGCTGCATCTGCAAGAGCAAATCTCTGGGTTCAAAGGTCAGTACTACCTAAAA CCAGGAATGGCAGTGCCATCCCCTACCTGTAATCCCAGCACTCCTGAGGTACAAGCAGGAAGATCAGGAA TGCAAAGTTACTGTAGCTGTAAGTTCGAAGCCAGCCTGAGCTAGTTGAGACCCCCTTACCTCAAAATAAA TTTAAGTTACAATTCTAAATTAAATTAAAATGTCTAGGGCTGGAGAGATGGCTTAGTGGTTAAGAGCACA GACTGCTTTTCCAGAGGTCCTGAGTTCAATTCCCAGCAACCACATGGTGGCTCACAACCATCTGTAATGG AATCTGATGCCCTCTTCTGGTATGTTTGAAGAGAGCGATGGTGTACTCATATACATAAAATAAGTAAATA AATCTTTTTTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5098695 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5098695 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11233 same embryo
	EMAGE:11232 same embryo
	EMAGE:11230 same embryo
	EMAGE:11231 same embryo
	EMAGE:11228 same embryo
	EurExpress:euxassay_003189 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source