EMAGE mouse gene expression spatial database

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EMAGE:11230

Akr1c13 aldo-keto reductase family 1, member C13 ( MGI:1351662)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003186. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003186_01	euxassay_003186_02	euxassay_003186_03	euxassay_003186_04

euxassay_003186_05	euxassay_003186_06	euxassay_003186_07	euxassay_003186_08	euxassay_003186_09

euxassay_003186_10	euxassay_003186_11	euxassay_003186_12	euxassay_003186_13	euxassay_003186_14

euxassay_003186_15	euxassay_003186_16	euxassay_003186_17	euxassay_003186_18	euxassay_003186_19

euxassay_003186_20	euxassay_003186_21	euxassay_003186_22	euxassay_003186_23	euxassay_003186_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11230_wholemount_strong.wlz

11230_wholemount_moderate.wlz

11230_wholemount_weak.wlz

11230_wholemount_possible.wlz

11230_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11230_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 11 12 13 14 15 16

foregut-midgut junction

strong

regional

strong expression: see section 16 18

pancreas

strong

regional

strong expression: see section 10 11 moderate expression: see section 12 13 14 15 19 20

stomach

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 moderate expression: see section 05 weak expression: see section 03 04

hindgut

strong

regional

strong expression: see section 14 15 16 17

rectum

strong

regional

strong expression: see section 16

midgut

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19 20 21 22 moderate expression: see section 23

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 14 15 moderate expression: see section 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4896
Entity Detected:	Akr1c13, aldo-keto reductase family 1, member C13 ( MGI:1351662)
Sequence:	sense strand is shown >T4896 GAATACTCAGGAAGTGCTTAGCCAGGGGAGTGAGAGACAATGAGCTCCAAACAGCACTGTGTCAAACTAA ATGATGGCCACTTAATTCCTGCCCTGGGCTTTGGCACCTATAAACCCAAGGAGGTTCCCAAGAGTAAGTC ACTGGAGGCTGCATGCCTAGCTCTAGATGTTGGGTACCGCCATGTTGATACTGCTTATGCATACCAAGTA GAAGAGGAGATAGGACAGGCCATTCAAAGCAAGATTAAAGCTGGGGTTGTAAAGAGAGAAGACCTGTTCG TCACTACAAAGCTTTGGTGCACTTGCTTTCGACCAGAGCTGGTCAAGCCTGCCTTGGAAAAGTCACTGAA AAAGCTTCAGCTGGATTATGTTGATCTTTACATTATGCATTACCCAGTGCCAATGAAGTCAGGGGATAAT GATTTTCCAGTAAATGAGCAAGGGAAATCTCTGTTGGACACTGTGGATTTCTGTGACACATGGGAGAGGT TGGAGGAGTGTAAGGATGCAGGATTGGTCAAGTCCATTGGGGTGTCCAACTTTAACCACAGGCAGCTGGA GCGAATCCTCAATAAGCCAGGACTGAAGTACAAACCTGTCTGCAACCAGGTTGAATGTCATCTCTATTTG AACCAGCGTAAGCTACTGGATTACTGCGAATCAAAAGACATTGTTCTTGTTGCTTACGGTGCTCTGGGGA CCCAGCGATATAAAGAATGGGTGGACCAGAACTCCCCAGTTCTCTTGAATGATCCAGTTCTTTGTGATGT GGCCAAAAAGAACAAGCGAAGTCCTGCCTTGATTGCACTTCGATACCTGATTCAACGTGGGATTGTGCCC CTGGCCCAGAGTTTCAAAGAGAATGAGATGAGAGAGAATTTGCAGGTTTTTGGATTTCAGTTGTCCCCTG AGGACATGAAAACACTAGATGGCCTGAACAAAAACTTTCGATACCTTCCAGCAGAGTTCCTTGTTGACCA CCCAGAGTATCCATTTGTGGAGGAATATTAACATGGGGACCTAATCATGGCTTCTGCCTGATGTCCCTGT GTGTGGACAGTGATGCTGGCAATATGACCAAGATGGACTGTTGGATGGACTTGTCATTTCTGATCAATCT TGGTTGCTTAGCAACTCACATTCAGCTGAAGCTTTAATTAATGATCTCAAAGAAATGGAATATAATTTTC ATGATGCTTTGAAATAAATATGAATTTTTCTCTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5100366 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5100366 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11233 same embryo
	EMAGE:11232 same embryo
	EMAGE:11229 same embryo
	EMAGE:11231 same embryo
	EMAGE:11228 same embryo
	EurExpress:euxassay_003186 same experiment
	MGI:4823072 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source