EMAGE mouse gene expression spatial database

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EMAGE:11291

Thop1 thimet oligopeptidase 1 ( MGI:1354165)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006611. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006611_01	euxassay_006611_02	euxassay_006611_03	euxassay_006611_04

euxassay_006611_05	euxassay_006611_06	euxassay_006611_07	euxassay_006611_08	euxassay_006611_09

euxassay_006611_10	euxassay_006611_11	euxassay_006611_12	euxassay_006611_13	euxassay_006611_14

euxassay_006611_15	euxassay_006611_16	euxassay_006611_17	euxassay_006611_18	euxassay_006611_19

euxassay_006611_20	euxassay_006611_21	euxassay_006611_22	euxassay_006611_23	euxassay_006611_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11291_wholemount_strong.wlz

11291_wholemount_moderate.wlz

11291_wholemount_weak.wlz

11291_wholemount_possible.wlz

11291_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11291_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

moderate

regional

moderate expression: see section 01 02 04 05 06 24 weak expression: see section 03 18 19 20 21 22 23

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7127
Entity Detected:	Thop1, thimet oligopeptidase 1 ( MGI:1354165)
Sequence:	sense strand is shown >T7127 CTCGGAGCTCGCGGGCGGCGGGCGCCTCAGTCTGAGAGCGACAGCGGAGGAACTGGCTCAGGTGAGGCGA GCTCCGAGGAGATCCCGACCGCACAGGCGTGAGGACCAGAGGAGGGCCGTCGCCGCCTCGCGCGCAGCCG ACCGCCATGAAGCCCCCCGCAGCCTGTGCTGGGGACGTGGTGGATGCAGCATCCCCAGCCTCCACTGTGA ATCATCTTCGCTGGGATCTGAGCGCACAGCAGATCAGGGCTCTAACCACGCAGCTCATCGAGCAGACCAA GTGTGTGTACGACCGAGTGGGTGCTCAGAACTTCGAGGATGTGTCGTATGAGAGCACACTGAAGGCACTG GCTGACGTGGAGGTCACCTACACAGTGCAGAGGAACATTCTCGACTTCCCCCAACACGTGTCCCCGTGCA AGGACATCCGTGCAGCCAGCACAGAGGCTGACAAGAAGCTCTCGGAGTTTGATGTAGAGATGAGCATGAG ACAGGACGTGTACCAGAGGGTCGTGTGGCTGCAGGAGAAAACCCCGAAAGATTCCCTGAAGCCTGAAGCA GCTCGCTACCTGGAGCGGCTCATCAAGCTGGGCCGGAGAAACGGCCTCCACTTACCTCAGGACACACAAG AGAAGATCAAGAACATCAAGAAGAGGCTGAGCCTGCTGTGCATCGACTTCAACAAGAACCTCAACGAGGA CACCACCTTCCTGCCCTTCACAAGAGAGGAGCTGGGTGGGCTCCCGGAGGACTTTCTGAGCTCTCTGGAG AAGGCAGAGGATGGCAAGCTGAAGGTCACCCTCAAGTACCCTCACTACTTCCCGCTGTTGAAGAAGTGCC ATGTGCCAGAGACACGACGCCTGTTGGAGGAGGCCTTCAACTGTCGCTGCAAGGAGGAGAACTGTGCAAT TCTGAAGGAGCTAGTGTCCCTGCGAGCACAGAAGTCCAGCCTGTTGGGGTTCCACACACACGCGGACTAC GTCCTGGAGATGAACATGGCCAAGACCAGTCAAACAGTAGCCACCTTCCTAGATGAGCTGGCACAGAAGC TGAAGCCCCTGGGTGAGCAGGAGCGCGCGGTCATCCTGGAGCTGAAGGAGGCAGAGTGCGCCAAGCGGGG ACTGCCATTCGACGGGCGCATCCACGCATGGGACATGCGCTACTACATGAACCAGGTGGAGGAGACTCGC TATCGTGTGGACCAGAACCTGCTGAAGGAGTACTTCCCCATGCAGGTGGTCACACGTGGGCTGCTGGCCA TCTACCAAGAGCTGCTGGGGCTGACCTTCACGCTGGAGGAAGGTGCCGCTGCCTGGCACGAGGACGTGCG GCTCTACTCAGTGCGGGACGCCGCCTCTGGAGAGGAGATCGGCAAGTTCTACCTCGACCTATACCCCAGG GAAGGGAAATATGGCCATGCAGCCTGCTTTGGCCTGCAGCCTGGCTGCCTACGGCAGGATGGCAGCCGGC AGCTGGCCGTCGCAGCCATGGTGGCCAATTTCACGAAGCCCACACCTGACGCGCCGTCCCTGCTGCAGCA TGATGAGGTGGAGACCTACTTCCACGAGTTCGGGCACGTCATGCACCAGCTGTGCTCGCAGGCGGAGTTT GCCATGTTCAGTGGGACCCACGTGGAGCGGGACTTTGTGGAGGCGCCGTCCCAGATGCTGGAGAACTGGG TGTGGGAGAAGGAGCCCCTGATGCGCATGTCTCAGCACTACCGCACGGGCAGCGAGGCCCCTCAGGACCT CCTGGAGAAGCTCATCAAGTCTCGCCAGGCCAATGCAGGTCTCTTCAACCTGCGGCAGATTGTGCTTGCC AAGGTGGATCAGGTCCTGCACACACAGACAGACGCAGACCCGGCTGAGGAATATGCCCGGCTCTGCCAAG AGATCCTCGGGGTGCCAGCCACACCAGGTACCAACATGCCAGCCACTTTCGGCCACCTAGCTGGCGGCTA TGATGCTCAGTACTATGGCTACTTATGGAGCGAAGTGTACTCGATGGACATGTTCCACACACGCTTCAAG CAAGAGGGTGTGCTTAGCCCCAAGGTTGGCATGGACTACAGGACCAGCATCCTGAGGCCAGGGGGCTCTG AGGACGCCAGCGCCATGCTGAAGCAGTTCCTGGGCCGCGACCCCAAGCAGGACGCCTTCCTCCTGAGCAA GGGTCTGCAGGTGGAGGGCAGCGAGGCGCCTGCGTGCTGACCGGTCCCCAGCTCGCCGCCCTGTGCCTTA GCCCAGCCTGCTGGGCAGCTCAGCAACCCTTGGGCGTACAGATGAGCTTGGGGAAGTTTCCATCCTTGTC CCACCCCGGCTCCAGGTCCCCTGGCCTCAGGCTGCCCAGTGGTCTCCTTGCACTAGCCCCTCCCCAACCT GATGTTGGACATTAAACTCCACACAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4019169 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4019169 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11293 same embryo
	EMAGE:11292 same embryo
	EMAGE:11290 same embryo
	EMAGE:11289 same embryo
	EMAGE:11294 same embryo
	EurExpress:euxassay_006611 same experiment
	MGI:4828702 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source