EMAGE mouse gene expression spatial database

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EMAGE:11293

Mamdc2 MAM domain containing 2 ( MGI:1918988)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006602. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006602_01	euxassay_006602_02	euxassay_006602_03	euxassay_006602_04

euxassay_006602_05	euxassay_006602_06	euxassay_006602_07	euxassay_006602_08	euxassay_006602_09

euxassay_006602_10	euxassay_006602_11	euxassay_006602_12	euxassay_006602_13	euxassay_006602_14

euxassay_006602_15	euxassay_006602_16	euxassay_006602_17	euxassay_006602_18	euxassay_006602_19

euxassay_006602_20	euxassay_006602_21	euxassay_006602_22	euxassay_006602_23	euxassay_006602_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11293_wholemount_strong.wlz

11293_wholemount_moderate.wlz

11293_wholemount_weak.wlz

11293_wholemount_possible.wlz

11293_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11293_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7126
Entity Detected:	Mamdc2, MAM domain containing 2 ( MGI:1918988)
Sequence:	sense strand is shown >T7126 GGCACCACTTGCTGACCTCTCCTCTCCCCCAAGTGGTGCCTGGCCCTTCTCAAGTGTGCAGTTGTCTCCT CCCTGTCCAGTCCGGCAGTCGCCCGGGGCCCAACCGGGCTACGAGTGGCCGGACGCTGCAGCCTTGCGCA GCGCGCTCTGCTCCTCAGACTCTTCGAATTTGAGCAGCCTGTGGCATCCCCCAGCAGGTCCCCAGCTCCT TGCCTAGCACCCTCCCTTCCCTAGGAGCAGCGGGCCACAGTGAGCCAGCAGCCCTCGCGGGTTCTCCTGC CTGAAGTTCAACTATGCTACTAGAAGGGGTCCTGCTGGTAGTGCAAGCCTTGCAGCTTGCCAGTGCCCTA GACCTGCCCGCTGGCTCCTGCGCCTTTGAAGAAGACACGTGTGGCTTTGACTCCGTGTTTGCGTTTCTGC CTTGGATACTAAATGAGGAAGGCCATTATGTCTATATGGACACCTCTTTTGCCAGGCAAGGGGAGAAAGC TGTGCTGCTGAGTTCTGATTTGCAGGCTGAGGAGTGGAACTGTTTGCGCCTGGTCTACCAGATAACCACA CCTCCAGGATCTGTGTCAGACCCCAGCCAGCTGAACCTCTATGTGAGATTTGAGGATGAGAGCTTCGATC GCTTGCTTTGGTCCACTAAGGAGCCTTCAGACAGCTGGCTCATAGCCAGCCTGGATCTTCAAAACACCTC CAAGAAATTCAAGATTTTGATAGAAGGTGTCCTGGGACAAGGAAACACGGCTAGCATTGCCCTCTTTGAA ATCAAGATGACAGCCGGCTACTGTATTGAATGTGACTTTGAAGAAAACCATCTCTGTGGCTTTGTGAACC GCTGGAATCCCAATGTGAACTGGTTCGTGGGAGGAGGAACCGCGAAGAACACCCACTCCGTCCTCCCACA GGATCACACCTTCAGGAGTGAGCACGGCCACTACATGTATGTGGACTCTGTGTATGTCAAGCACTTCCAG GAAGTGGCCCAGCTCATCTCCCCAGTGACCACAGCCTCCATGTCTGGCTGTCTCTCATTCTATTACCAGC TTCAGCAGGGGAATGACAATGTCTTCTCTGTTTACACGCGGGACATGGCTGGCCTGTACGAGGAGATCTG GAAAGTGGACAGCCCAGGCAATGCGGCCTGGAACCTTGCGGAGGTGGAATTTAGTGCTCCTTACCCCATG GAGGTTATTTTTGAAGTTGCTTTCAATGGTCCCAAAGGAGGCTACGTTGCCCTGGATGATATTTCTTTCT CTCCTGTTCACTGCCAAAATCAGACAGGGCTTCCTTTCAGTGCTGTGGAAACCAGCTGCGATTTTGAGAT AGGCCTCTGCAACTTTTATCAAGACAAAGAGGGTCCGGGTTGGACCAGAGTAAGAGTAAAAGCAAACATG TATCGGGCCGGAGACCACACGACAGGAACAGGGCACTACCTGCTGGCCAACACTAAGTTTACATCCCAGC CTGGCTACATCGGAAGACTTTATGGTCCCTCTCTCCCTGGAAACATGCAGTACTGCGTGCGCTTTCATTA TGCCATCTTTGGGTTTTTGAAAATGAGTGACACCCTGGCCGTTTATATCTTTGAAGAAAACCATGTGGTT CAAGAGAAGATCTGGTCTGTGCTCGAGTCCCCGAGGGGTGTTTGGATGCAAGCGGAAATCAGTTTCAAGA AGCCTATGCCCACAAAGGTGGTTTTTATGAGCCTGTGCAAAAGCTTTTGGGACTGTGGCCTGGTAGCCCT GGATGACATTACCATACAACTAGGAAACTGCCGGTCTCCAGCAAGACTTCCACCTCCACCTGGAGAATGC ACTTTTGATCAAGATGAATGTGCGTTTACACAGGAGAAAAGGAACCGAAGCAGCTGGCACAGGGGAAGGG GAGAAACGCCCACCTCCTACACAGGACCCAAGGGAGATCACACTACTGGGGTAGGTTACTACATGTACAT CGAAGCCTCCCACATGGTGTATGGACAGAAAGCACACCTTTTGTCCCAGCCTCTGAGAGGCGTCCCTGGG AAACACTGCTTGACGTTTTTCTACCACATGTACGGAGCAGGGACTGGCCTGCTGAGTGTTTATTTGAAAA GGGAAGAAGATAGTGAGGAGTCCCTGTTGTGGAGAAGAAGGGGAGAGCAGAGCATCTCCTGGCTGCGGGC ACTGGTTGAATACAGCTGCAGGAGGAGACACCAGATAATTTTTGAAGCCACTCGAGGAGTGTCCATAAGA AGCGATATCGCCATTGATGATGTTAAACTTCAGGCAGGGCCATGTGCAGGAATGGAAGATACAACTGAAC AGTCATCAGGATATTCTGAAGACTTAAATGAAATCGAGTATTAAGAAATGATCTAACTTAGAGTAAGAAG ACAAAAGTTATACCTCTCTTCAGTCAAAGAGGAAACAAAAGAAATGGATACTGGATGCTCTTGACTATTG CATAAGTTGAAATGACTTCAGAAGACCCGCTCTCGTTACTTTTGAAAACTATATCTCTATGTATTAACTC AGGGCTCTCCTCTGTTTTCCCCCCAGCACATCTGACAACTGTTACTAGAAGTACAGGCTACTGGTTTTAC AGAAACCATTTCATCTTAATTTTGGTGCCAGTTAAAATACGAATGTACTGTGTTACGGTCACTTCAAAGT ACACGTATAAAGGGCACAATCAGAGCGAGTGTGCTTATAAGCCTGCATTCAGTGAATGTATTGGGAGACT AGGTCTTGGTGGTGTTCCTTTTGAGTTTCAAGAATGAGAAGTTTTCTTAAATAGTAATGCAAGGTCAGTA ATGTCCGCCAAAGGGATGCAGTGTTTTATGGTAACAATTATTCTGGAAATAAGGTCGCCCCATGGAGCCC CAGGATAAGTCTGCGCTAATTCATGCTGCCTTACAGGGCAGTGCCACCTTTTCTTCTCCATTTCTACTGC CTTGGACAGTCACCAGGAGCACCTGCATAGCAATGCACCATTTCAACAAGGCAAGCTTGAATTATTTTAT GCACATACACTCAACAATTTTTATAAATGTGTTAAACAATTTGACCCATTTTTATCATAAATATTTTTAG TGAAATTTTTAGTAGTATGAGTTCAACCACATTTGATAAACTATGGTTTTTTTATTGACTTATTTAGAAT TGTATATATGTGTGCAGTTGTGTGGCTAATCAGCTGTCTAAAACTTTAAAATTGCCTAATAAAAGATCAT TTAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4019075 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4019075 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11292 same embryo
	EMAGE:11291 same embryo
	EMAGE:11290 same embryo
	EMAGE:11289 same embryo
	EMAGE:11294 same embryo
	EurExpress:euxassay_006602 same experiment
	MGI:4826070 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source