EMAGE mouse gene expression spatial database

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EMAGE:11307

Clptm1 cleft lip and palate associated transmembrane protein 1 ( MGI:1927155)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006630. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006630_01	euxassay_006630_02	euxassay_006630_03	euxassay_006630_04

euxassay_006630_05	euxassay_006630_06	euxassay_006630_07	euxassay_006630_08	euxassay_006630_09

euxassay_006630_10	euxassay_006630_11	euxassay_006630_12	euxassay_006630_13	euxassay_006630_14

euxassay_006630_15	euxassay_006630_16	euxassay_006630_17	euxassay_006630_18	euxassay_006630_19

euxassay_006630_20	euxassay_006630_21	euxassay_006630_22	euxassay_006630_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11307_wholemount_strong.wlz

11307_wholemount_moderate.wlz

11307_wholemount_weak.wlz

11307_wholemount_possible.wlz

11307_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11307_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7379
Entity Detected:	Clptm1, cleft lip and palate associated transmembrane protein 1 ( MGI:1927155)
Sequence:	sense strand is shown >T7379 CCACGCGTCCGGGAAGATGGCGGCGGCGCAGGAGGCGGACGGGGCCGGCAGCGCCGTGGTGGCGGCCGGG GGAGGCAGCTCTGGTCAGGTGACCAGCAATGGCAGCATTGGGAGGGACACGCCAGCCGAGACCCAGCCTC AGAACCCTCCACCCCAGCCAGCCCCCAACGCCTGGCAGGTCATCAAAGGCGTTCTGTTCAGGATCTTCAT CATCTGGGCCATCAGCAGCTGGTTCCGCCGAGGGCCGTCCCCTCAGGACCAGTCAGGCCCTGGAGGGGCC CCACGTGTCGCCAGCCGAAACCTATTCCCCAAAGACACTCTAATGAACCTGCACGTGTACATCTCAGAGC ACGAGCACTTCACAGACTTCAACGCCACCTCGGCGCTCTTCTGGGAGCAGCATGACCTGGTGTATGGTGA CTGGACCAGTGGAGAGAACTCTGATGGCTGCTACGAGCACTTTGCAGAGCTTGACATCCCACAGAGTGTA CAGCAGAATGGCTCCATCTATATCCATGTCTACTTCACTAAGAGTGGCTTCCATCCAGACCCCCGGCAGA AGGCCCTGTACCGCCGGCTTGCCACTGTCCACATGTCCCGGATGATCAACAAATACAAGCGTAGGCGATT TCAGAAAACCAAGAACCTACTGACAGGAGAGACAGAAGCTGACCCAGAAATGATCAAGAGGGCTGAGGAC TATGGCCCTGTAGAAGTAATCTCGCACTGGCACCCCAACATCACCATCAACATCGTGGATGACCACACAC CATGGGTGAAGGGCAGTGTGCCACCCCCACTGGACCAATATGTGAAGTTTGATGCCGTGAGTGGTGACTA CTACCCCATCATCTATTTCAATGACTACTGGAACCTGCAGAAGGATTACTACCCCATTAATGAGAGTCTG GCCAGCCTACCACTGCGTGTCTCTTTCTGCCCGCTGTCACTATGGCGCTGGCAACTCTATGCTGCACAGA GTACCAAGTCACCCTGGAACTTCTTAGGTGACGAGCTGTATGAGCAATCGGATGAGGAGCAGGATTCAGT GAAGGTTGCTCTGCTGGAAACCAGCCCGTATCTTCTGGCACTCACCATCATCGTCTCCATTGTCCACAGT GTCTTTGAGTTCCTGGCTTTCAAAAATGACATTCAGTTCTGGAACAGTCGTCAGTCCTTGGAGGGGCTGT CAGTGCGCTCCGTCTTCTTTGGCGTTTTCCAGTCCTTTGTGGTTCTCCTTTACATCCTGGACAATGAGAC CAACTTTGTGGTACAGGTCAGCGTCTTCATTGGGGTCCTCATCGACCTCTGGAAGATTACCAAAGTCATG GACGTCCGGCTGGACCGGGAGCATAGAGTGGCAGGAATCTTCCCTTGCCCAACCTTCAAAGACAAGTCCA CCTATATTGAATCTTCAACCAAAGTATATGATGATATGGCGTTCCGGTACCTGTCATGGATCCTCTTTCC CTTGCTGGGCTGCTATGCCGTCTACAGCCTTCTGTACCTGGAGCACAAGGGCTGGTACTCCTGGGTGCTC AGCATGCTCTACGGTTTCCTGCTGACCTTTGGCTTCATCACTATGACACCACAGCTCTTCATCAACTATA AGCTCAAGTCTGTGGCCCATTTGCCCTGGCGGATGCTCACCTACAAGGCCCTCAACACTTTCATTGATGA CTTATTTGCCTTTGTCATCAAGATGCCTGTCATGTACCGGATCGGCTGCCTGCGGGATGATGTGGTTTTC TTCATCTACCTCTACCAACGGTGGATCTACCGCGTCGACCCCACCCGTGTGAACGAGTTCGGTATGAGTG GAGAGGATGTCTCAGCAGCGGCATCCAGGGCCCAGGCATCCACTGCAGCAGGGGCTCTCACACCTGCACC CAGCACAGCTGTCAGCGGAGAGGATGCCTCCACAGTCCCAAAGGCCACTTCTGGGGCCTGCACTGCCAGC CAGCCCCAGGAAGCCCCTCCAAAGCCAGCAGAGGACAAGAAAAAGGATTAGCCACTGCAGTCCTCCTTAT CTGCTCCTGGTGACTGACCACCACCACCTCCCGGGCCCCTCCCTTACCCTCTCTGTCGCCCTCTTCCTGG ACAGATCAGGCCGGGGCGGCAGGAGGCCCCCAGGGCCAGGGCCCGGTGTGTGAGATGGGGCCGGGCAGGT TGGGGCTGGCTGTGTGTATGTGGAGGCACCGTCCATCGTCTTGTCTATCCGTCTGTTCCTATGTGTTTGC AGACATCTCGCCCTGCCAGCCCAACACCATCGGGAATCATGGTGAAGCCAATATGGCGCCGAGGGTGGGC CAGGCGGGGAAGGGGCCAGGGCTCCTCTGTGGGACGCCTGTGGTCCTCCATCATCTTGTCCCTCATCCCC CGACCACCCTGCTCCCAGACCGCCACCCTTTAACACAGTCTGGATTTAATAAATTCATATGGGTGTTTAA CTTAAGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4193279 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4193279 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11309 same embryo
	EMAGE:11310 same embryo
	EMAGE:11308 same embryo
	EMAGE:11312 same embryo
	EMAGE:11311 same embryo
	EurExpress:euxassay_006630 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source