EMAGE mouse gene expression spatial database

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EMAGE:11329

Gsdmd gasdermin D ( MGI:1916396)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003539. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003539_01	euxassay_003539_02	euxassay_003539_03	euxassay_003539_04

euxassay_003539_05	euxassay_003539_06	euxassay_003539_07	euxassay_003539_08	euxassay_003539_09

euxassay_003539_10	euxassay_003539_11	euxassay_003539_12	euxassay_003539_13	euxassay_003539_14

euxassay_003539_15	euxassay_003539_16	euxassay_003539_17	euxassay_003539_18	euxassay_003539_19

euxassay_003539_20	euxassay_003539_21	euxassay_003539_22	euxassay_003539_23	euxassay_003539_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11329_wholemount_strong.wlz

11329_wholemount_moderate.wlz

11329_wholemount_weak.wlz

11329_wholemount_possible.wlz

11329_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11329_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 13 14 15 weak expression: see section 16 17

oral region

moderate

regional

moderate expression: see section 11

hindgut

moderate

regional

moderate expression: see section 14 15 16 17 18

rectum

moderate

regional

moderate expression: see section 15 16

midgut

moderate

regional

moderate expression: see section 09 10 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7405
Entity Detected:	Gsdmd, gasdermin D ( MGI:1916396)
Sequence:	sense strand is shown >T7405 CCGGGTTGAGCAGACAATAGACCCCTCCCCGGCATCCCAGCAGGTCCTCGCTTCGCTTGGTGGACCCAGA TACCTCGGCAGGGGTGAAAAATCGAGGACCATGCCATCGGCCTTTGAGAAAGTGGTCAAGAATGTGATCA AGGAGGTAAGCGGCAGCAGAGGCGATCTCATTCCGGTGGACAGCCTGCGGAACTCCACCAGCTTCAGGCC CTACTGCCTTCTGAACAGGAAATTTTCAAGCTCAAGGTTCTGGAAACCCCGTTATTCATGTGTCAACCTG TCAATCAAGGACATCCTGGAGCCCAGTGCTCCAGAACCAGAACCGGAGTGTTTTGGCTCCTTCAAAGTCT CTGATGTCGTCGATGGGAACATTCAGGGCAGAGTGATGTTGTCAGGCATGGGAGAAGGGAAAATTTCTGG TGGGGCTGCAGTGTCTGACAGTTCCAGTGCCTCCATGAATGTGTGTATACTGCGTGTGACTCAGAAGACC TGGGAGACCATGCAGCATGAAAGGCACCTTCAGCAGCCTGAGAACAAAATCCTGCAACAGCTTCGGAGTC GTGGGGATGACCTGTTTGTGGTGACCGAGGTGCTGCAGACAAAGGAGGAAGTGCAGATCACTGAGGTCCA CAGCCAAGAGGGCTCAGGCCAGTTTACGCTGCCTGGAGCTTTATGCTTGAAGGGTGAAGGCAAGGGCCAC CAAAGCCGGAAGAAGATGGTGACCATTCCTGCAGGCAGCATCCTGGCATTCCGAGTGGCCCAACTGCTTA TTGGCTCTAAATGGGATATCCTTCTCGTCTCAGATGAGAAACAGAGGACCTTTGAGCCCTCCTCAGGTGA CAGAAAAGCAGTGGGCCAGAGGCACCATGGCCTCAATGTGCTTGCTGCGCTTTGTTCCATCGGAAAGCAG CTCAGTCTCCTGTCAGATGGGATTGATGAGGAGGAATTAATTGAGGCGGCAGACTTCCAGGGCCTGTATG CTGAGGTGAAGGCTTGCTCCTCAGAACTGGAGAGCTTGGAAATGGAGTTGAGACAACAGATACTGGTGAA CATCGGAAAGATTTTACAGGACCAGCCCAGCATGGAAGCCTTAGAGGCCTCACTAGGGCAGGGCCTGTGC AGTGGCGGCCAGGTGGAGCCTCTGGACGGCCCAGCTGGCTGCATCCTTGAGTGTCTGGTGCTTGACTCTG GAGAACTGGTGCCGGAACTCGCAGCCCCTATCTTCTACCTGCTGGGAGCACTGGCTGTGCTGAGTGAAAC CCAGCAGCAGCTGCTAGCTAAGGCTCTGGAGACAACGGTGCTGTCAAAGCAGCTGGAGTTGGTGAAGCAC GTCTTGGAACAGAGCACCCCGTGGCAGGAGCAGAGTTCTGTGTCCCTGCCCACCGTGCTCCTTGGGGACT GCTGGGATGAAAAGAATCCCACCTGGGTCTTGCTAGAAGAATGTGGCCTAAGGCTGCAGGTAGAATCCCC CCAGGTGCACTGGGAACCAACGTCTCTGATCCCCACAAGTGCGCTCTATGCCTCCCTGTTCCTATTGTCA AGTCTAGGCCAGAAACCTTGTTAGCCTGTGGGCCTCCCTTCCCACAACATCTCCATGTCCTACCCTCCAG CCAAGGTAGAATCTTGCCAAGCCTAGCCTTTGGGAAGCCAAGAACCATACTCAGTCACAGGGTTATAATG CACTGAGATCCAGAAGTTGGGAAAACTCAATAAATGTACAAAGGAAAGCATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4194837 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4194837 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11330 same embryo
	EMAGE:11334 same embryo
	EMAGE:11332 same embryo
	EMAGE:11333 same embryo
	EMAGE:11331 same embryo
	EurExpress:euxassay_003539 same experiment
	MGI:4825252 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source