EMAGE mouse gene expression spatial database

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EMAGE:11332

Cyp2d13 cytochrome P450, family 2, subfamily d, polypeptide 13 ( MGI:88605)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003533. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003533_01	euxassay_003533_02	euxassay_003533_03	euxassay_003533_04

euxassay_003533_05	euxassay_003533_06	euxassay_003533_07	euxassay_003533_08	euxassay_003533_09

euxassay_003533_10	euxassay_003533_11	euxassay_003533_12	euxassay_003533_13	euxassay_003533_14

euxassay_003533_15	euxassay_003533_16	euxassay_003533_17	euxassay_003533_18	euxassay_003533_19

euxassay_003533_20	euxassay_003533_21	euxassay_003533_22	euxassay_003533_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11332_wholemount_strong.wlz

11332_wholemount_moderate.wlz

11332_wholemount_weak.wlz

11332_wholemount_possible.wlz

11332_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11332_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 19 20 21 22 23

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7406
Entity Detected:	Cyp2d13, cytochrome P450, family 2, subfamily d, polypeptide 13 ( MGI:88605)
Sequence:	sense strand is shown >T7406 ATTGAGTGTTGTTCATTGGCCTAGGTGAAGCCTGGGAAGCAGTGGGGCAGCCATGGAGCTGCTGACTGGG ACTGGCCTGTGGCCTGTGGCCATATTCACAGTCATCTTCATATTACTGGTGGACCTGATGCACCGGCGCC AGCGCTGGACTTCTCGCTACCCACCGGGCCCTGTGCCATGGCCTGTGCTGGGTAACCTGCTGCAGGTGGA CCTGGATAACATGCCATACAGCTTGTACAAGCTTCAAAACCGCTATGGTGATGTGTTCAGCCTTCAGATG GCCTGGAAGCCCGTGGTTGTGATCAGCGGACTGAAGGCCGTGCGGGAAGTGCTGGTGACCTGTGGAGAGG ACACAGCTGACCGCCCTGAAATGCCCATCTTTCAACACCTAGGCTATGGGGAAAAAGCTAAAGGTGTGGT CTTTGCACCTTACGGGCCTGAATGGCGAGAACTGAGGAGATTCTCTGTGTCAACCCTGCGCAACTTGGGC CTGGGCAAGAAATCGCTGGAGCAGTGGGTGACAGAGGAAGCTGGCCACCTCTGTGATGCCTTCACCGCCC AGGCTGGGAGTCCCCTCGATCCTTACACACTCCTGAACAAAGCTGTGTGCAATGTGATTGCGTCTCTCAT TTATGCCCGTCGCTTTGAGTATGGAGACCCTGACTTCATCAAGATGCTAAAAATATTGAAAGAAAACATG GGAGAGAATACTGGCTTGTTTCCAGAGGTTCTGAACACATTTCCAATTCTCCTGCACATCCCAGGGTTGG CTGACAAAGTCTTCCCTGGGCAAAAGACATTTCTCACCCTGGTAAATAAGCTGGTGACTGAGCACAAGAG GACCTGGGACCCTGACCAGCCACCTCGTGACCTGACTGATGCCTTTCTGGCTGAAATGGAGAAGGCAAAG GGGAACCCTAAGAGTAGCTTCAATGAAGCAAATCTACGTCTGGTTGTCTTTGACCTGTTTGGTGCTGGGA TTGTGACCAGTTCAATCACTCTGACCTGGGCCCTGCTGCTCATGATCTTGCATCCAGATGTGCAGCGCCG AGTGCAAGAGGAAATCGACGAGGTCATAGGGCAGGTGCGGTGTCCAGAGATGGCAGACCAGGCCCACATG CCCTACACCAATGCTGTCATTCATGAGGTGCAGCGCTTTGCAGACATTGTCCCAATGAACTTGCCACACA AGACTTCTCATGATATTGAAGTACAGGGCTTCCTTATCCCTAAGGGGACGACCCTCATCCCCAACCTGTC CTCCACGCTAAAGGATGAGACTGTCTGGGAGAAGCCCCTCCGCTTCCATCCTGAACACTTCCTGGATGCC CAGGGCCATTTTGTGAAGCCTGAGGCCTTCATGCCATTCTCAGCAGGTCGTCGAGCATGCCTGGGGGAGC CCCTGGCCCGCATGGAGCTCTTCCTCTTCTTCACCTGCCTCCTGCAGCGCTTTAGCTTCTTAGTGCCTGC TGGACAGCCCCAGCCCAGTGACTATGGCATCTTTACATTTCTGGTTAGCCCCTCCCCCTATCAGCTCTGT GCATTCACACGTTAGCAGGCAACAAATCCTAGTCCAGAAACTAGCTTAGAGTCACACGCACAATAAACCA GTTTTGTGGCTGCCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4194968 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4194968 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11330 same embryo
	EMAGE:11334 same embryo
	EMAGE:11329 same embryo
	EMAGE:11333 same embryo
	EMAGE:11331 same embryo
	EurExpress:euxassay_003533 same experiment
	MGI:4824168 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source