EMAGE mouse gene expression spatial database

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EMAGE:11344

Vps26a vacuolar protein sorting 26 homolog A (yeast) ( MGI:1353654)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003555. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003555_01	euxassay_003555_02	euxassay_003555_03	euxassay_003555_04

euxassay_003555_05	euxassay_003555_06	euxassay_003555_07	euxassay_003555_08	euxassay_003555_09

euxassay_003555_10	euxassay_003555_11	euxassay_003555_12	euxassay_003555_13	euxassay_003555_14

euxassay_003555_15	euxassay_003555_16	euxassay_003555_17	euxassay_003555_18	euxassay_003555_19

euxassay_003555_20	euxassay_003555_21	euxassay_003555_22	euxassay_003555_23	euxassay_003555_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11344_wholemount_strong.wlz

11344_wholemount_moderate.wlz

11344_wholemount_weak.wlz

11344_wholemount_possible.wlz

11344_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11344_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5982
Entity Detected:	Vps26a, vacuolar protein sorting 26 homolog A (yeast) ( MGI:1353654)
Sequence:	sense strand is shown >T5982 GGCACCCAGGCTGGGAGTTCTTCTGAGGGAAAGCCGAGTGGAGTGGGCGACCCGGCGGCGGTGACAATGA GTTTTCTTGGAGGCTTTTTTGGTCCCATTTGTGAGATTGATGTTGCCCTTAATGATGGGGAAACCAGGAA AATGGCAGAAATGAAAACTGAGGATGGCAAAGTAGAAAAACACTATCTCTTCTATGATGGCGAGTCTGTC TCAGGAAAGGTAAACCTAGCCTTTAAGCAGCCTGGAAAGAGGCTAGAGCATCAAGGAATTAGAATTGAAT TTGTAGGTCAAATTGAGCTTTTCAATGACAAGAGTAATACTCATGAATTTGTAAACCTAGTGAAGGAACT AGCCTTGCCTGGAGAACTGACTCAGAGCAGAAGCTATGACTTTGAATTTATGCAAGTTGAAAAGCCATAT GAGTCATACATCGGTGCCAATGTCCGCCTGAGGTATTTTCTTAAGGTGACAATTGTGAGAAGATTGACAG ACTTAGTGAAAGAGTACGATCTTATTGTTCATCAGCTAGCCACCTATCCTGATGTCAACAACTCTATTAA AATGGAAGTGGGCATTGAAGACTGTCTGCACATAGAGTTTGAATATAATAAGTCCAAGTATCATTTAAAG GATGTAATTGTTGGAAAAATTTACTTCTTATTAGTAAGAATAAAAATACAACACATGGAATTACAGCTGA TCAAGAAAGAGATCACAGGAATTGGACCCAGCACCACAACAGAGACAGAAACAATCGCTAAGTATGAAAT AATGGATGGGGCGCCAGTAAAAGGAGAATCTATTCCGATAAGATTGTTCTTAGCAGGGTATGACCCAACC CCCACGATGAGAGATGTGAACAAGAAGTTTTCAGTAAGGTACTTTCTAAACCTCGTGCTTGTTGATGAGG AGGACCGAAGGTACTTCAAGCAGCAGGAGATCATCCTGTGGAGAAAAGCACCCGAGAAACTGAGAAAACA GAGGACGAACTTTCACCAGCGGTTTGAATCTCCAGACTCGCAGGCCTCTGCGGAGCAGCCTGAGATGTAA GCAGGTGGGCAGAGCAGCAAGTGCAAGAACCGGGCCAAGAGAGCAGCTCAGCTGGCTGACGACAGTCGCA GCGTGCGGACATCCCAACTCCACGTCTGCTAGTCTTCCTTATCCTAGAGTGCTGCGTTTATTTTACAGTA TGATTAAAGGTTTTGTGTATATACATTTTAAAAAGTGCTTCTTGGAAACACTGGAGAATTCCTAAGCTGC CTTTTTTGTTTTCTTTTTTACTGCACACTGATAAGCACTCAGAAGCATAAACATTAAGGATTTTCATGTG AGTAGACTGGTATTTGTAATTTTGCTTTCTTTATGCATAATGTTGAAAATCCTTTTCTCTGTTTTCATGC TAATGATTACCTCTTATTCTCTATGTGCAAAAAATTAAATATTTTTGTTCAAGTAAAATGAGAAAAACCT GAGCAGCCCTTATGTCCTACAAAGTTTTAAGGCATGGCTCCTCAGTATTTTTGAGAATTGTGTTTATTTT TACATGTGGATCAGAAACCAACCTGGTGTTACATGGTAGGCACTTCTGGGTCTACCATTCACAGCCTCCT GAATTTTAATCTTTGGGATCTCTGAGGTATATGAAAGATTGTCACCCCAGGGATGACATGGTTTGTCATC GTGGCCCTCTTGCCCCTTATTGACCACGTTTGCTATGACTTTCTGTATCATCATGTAGGTTCTGCAGTGT GGAGTTGACACATTGGCATTTTAGAACTTGTTAAACCTGTATCATTTTTTTTCCATAAAGACTTTGAAAC ATGTATATATTTTTTAATTTAAGGACTACTTTCCATGTGTATGCAAATCGTCACCTAGGAATGAGAGAGC TTGGGAGAACCAGCCGAAGTGTCCATAGCCGGACAATTTCTTGTTAACTGGAATATTGGCTAGTTCAAAC TTGACTTCACGTAGTCTATTTAAAAAAACCCGAACCAAACAGAACAAAAAACACTAAATTCATACCATTC TCTGAAAAGAAGGCTGCAGTCAGCTGAGCACAGACACACAACTGAGGCGTATGGGGCAGGAGGAAGCCTG TGTGGGGGCCACTCTCACTCAGGGCTTGTTGGTTTCTGTACCAGTGTTCCTTGATACACCTCAGTACAGG TTTGTCAGTTAACTTTACTTTCTAGGAAGCTCAACAGCCCAACTTATGTTTCCTTAAACCTATTTTTTAC TATGATGGCACTTTGATAAAATGGCCAGCAACCAGCCCTGGCCAAAGGTTCACATACCACGTCCTGGAGC AGCTATTGTCAATGTGGGTTTCTAAGGACGGCCATGTTCTCCTTCATGTAAGTGCCTGTTCAGAGTCTCA AAGTTTCAAAATGCCAAATATTTTCATGGTCACTTGCATGTATAATCTAGGAAATATTCAAGGAGATTTT GAAAAACAATTGTATTTAACCAGCCTCAAGTTGTGCAACCATGATATATAAAATAAATAATTTGAAACAG AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3485844 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3485844 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11347 same embryo
	EMAGE:11343 same embryo
	EMAGE:11348 same embryo
	EMAGE:11346 same embryo
	EMAGE:11345 same embryo
	EurExpress:euxassay_003555 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source