EMAGE mouse gene expression spatial database

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EMAGE:11348

Ly6a lymphocyte antigen 6 complex, locus A ( MGI:107527)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003542. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003542_01	euxassay_003542_02	euxassay_003542_03	euxassay_003542_04

euxassay_003542_05	euxassay_003542_06	euxassay_003542_07	euxassay_003542_08	euxassay_003542_09

euxassay_003542_10	euxassay_003542_11	euxassay_003542_12	euxassay_003542_13	euxassay_003542_14

euxassay_003542_15	euxassay_003542_16	euxassay_003542_17	euxassay_003542_18	euxassay_003542_19

euxassay_003542_20	euxassay_003542_21	euxassay_003542_22	euxassay_003542_23	euxassay_003542_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11348_wholemount_strong.wlz

11348_wholemount_moderate.wlz

11348_wholemount_weak.wlz

11348_wholemount_possible.wlz

11348_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11348_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

physiological umbilical hernia

moderate

regional

moderate expression: see section 12

kidney calyx

strong

regional

strong expression: see section 08 09 10 19 20 21 moderate expression: see section 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5986
Entity Detected:	Ly6a, lymphocyte antigen 6 complex, locus A ( MGI:107527)
Sequence:	sense strand is shown >T5986 CTGAGAGGAAGTTTTATCTGTGCAGCCCTTCTCTGAGGATGGACACTTCTCACACTACAAAGTCCTGTTT GCTGATTCTTCTTGTGGCCCTACTGTGTGCAGAAAGAGCTCAGGGACTGGAGTGTTACCAGTGCTATGGA GTCCCATTTGAGACTTCTTGCCCATCAATTACCTGCCCCTACCCTGATGGAGTCTGTGTTACTCAGGAGG CAGCAGTTATTGTGGATTCTCAAACAAGGAAAGTAAAGAACAATCTTTGCTTACCCATCTGCCCTCCTAA TATTGAAAGTATGGAGATCCTGGGTACTAAGGTCAACGTGAAGACTTCCTGTTGCCAGGAAGACCTCTGC AATGTAGCAGTTCCCAATGGAGGCAGCACCTGGACCATGGCAGGGGTGCTTCTGTTCAGCCTGAGCTCAG TCCTCCTGCAGACCTTGCTCTGATGGTCCTCCCAATGACCTCCACCCTTGTCCTTTTATCCTCATGTGCA ACAATTCTTCCTGGAGCCCTCTAGTGATGAATTATGAGTTATAGAAGCTCCAAGGTGGGAGTAGTGTGTG AAATACCATGTTTTGCCTTTATAGCCCCTGCTGGGTAGGTAGGTGCTCTAATCCTCTCTAGGGCTTTCAA GTCTGTACTTCCTAGAATGTCATTTTGTTGTGGATTGCTGCTCATGACCCTGGAGGCACACAGCCAGCAC AGTGAAGAGGCAGAATTCCAAGGTATTATGCTATCACCATCCACACATAAGTATCTGGGGTCCTGCAATG TTCCCACATGTATCCTGAATGTCCCCCTGTTGAGTCCAATAAACCCTTTGTTCTCCCAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:3486025 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3486025 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11347 same embryo
	EMAGE:11343 same embryo
	EMAGE:11346 same embryo
	EMAGE:11345 same embryo
	EMAGE:11344 same embryo
	EurExpress:euxassay_003542 same experiment
	MGI:4826027 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source