EMAGE mouse gene expression spatial database

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EMAGE:11376

Rab4b RAB4B, member RAS oncogene family ( MGI:105071)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006754. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006754_01	euxassay_006754_02	euxassay_006754_03	euxassay_006754_04

euxassay_006754_05	euxassay_006754_06	euxassay_006754_07	euxassay_006754_08	euxassay_006754_09

euxassay_006754_10	euxassay_006754_11	euxassay_006754_12	euxassay_006754_13	euxassay_006754_14

euxassay_006754_15	euxassay_006754_16	euxassay_006754_17	euxassay_006754_18	euxassay_006754_19

euxassay_006754_20	euxassay_006754_21	euxassay_006754_22	euxassay_006754_23	euxassay_006754_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11376_wholemount_strong.wlz

11376_wholemount_moderate.wlz

11376_wholemount_weak.wlz

11376_wholemount_possible.wlz

11376_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11376_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 02 03 04 05 06 07 08 18 19 20 21 22 23 24

nasal septum

strong

regional

strong expression: see section 15

viscerocranium

strong

regional

Expression in the turbinate bone.

mandible

strong

regional

strong expression: see section 06 07 08 09 10 13 17 18 19 20 21 22

maxilla

strong

regional

strong expression: see section 07 08 09 10 13 19 20 21 22

clavicle

strong

regional

strong expression: see section 08 09 10 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8168
Entity Detected:	Rab4b, RAB4B, member RAS oncogene family ( MGI:105071)
Sequence:	sense strand is shown >T8168 GGCACGAGGCCGGGTCCAGAAGTGGAAAGGAGCTGAGGCTGCAGCCCGAGTGGAGCGGCTGCCAGCCGAG GAACAGGCGCGGCTGCGGCGCCATATTGCGGCCCTCAGCGGTCGCCACTGAGCCATGGCCGAGACCTACG ACTTCCTCTTCAAATTCCTGGTGATTGGCAGTGCTGGAACTGGCAAATCATGTCTTCTCCACCAGTTTAT TGAGAATAAGTTCAAACAGGACTCCAACCACACTATCGGCGTGGAGTTTGGATCCAGGGTGGTCAACGTT GGGGGGAAGACTGTGAAACTACAGATTTGGGACACTGCTGGCCAGGAGCGGTTTCGGTCGGTGACGCGGA GTTATTACCGAGGGGCAGCTGGAGCCCTGCTGGTGTATGACATCACCAGCCGGGAGACATACAACTCACT CGCTGCTTGGCTAACTGATGCCCGGACACTGGCCAGCCCCAACATCGTGGTCATCCTCTGTGGCAACAAG AAGGACCTGGATCCCGAGCGTGAGGTCACTTTCCTAGAGGCTTCTCGCTTTGCTCAGGAGAATGAGCTGA TGTTCCTGGAGACCAGCGCTCTCACGGGTGAGAATGTGGAAGAAGCTTTCCTCAAGTGCGCGCGCACCAT CCTCAACAAGATCGACTCAGGTGAACTAGACCCAGAGAGGATGGGTTCAGGCATTCAGTATGGCGACATA TCCCTCCGCCAGCTGCGGCAGCCTCGCAGCGCCCAAGCCGTGGCCCCTCAGCCCTGTGGCTGCTGAGATG TGCAGAACCAGCTCACCTGTTCTTCTGGACCAGCCCTGCCCGAAGGCTGGGGCTCAGAACCAGGCCCCAA GAGGCCAGGCCCCAATCTGCCCCAGTCTCAGAAAAGTTACTCCATCCCCCATCCCCCTTCGCTGGCCTGG TGGGGCCTGGCTGTGGGGCAAGATTGAGCCACAGGGGAAAGGGGACCCCATACCTGCTGCTGCTTCCTCT GCCTTGGCTAACTCTAGTCCCCCTGAGCCCCAAACCCTACCTGAAGAACTCCCAAAGCTTGAGACCAGGG CCGTTTGGCCCCAACTTCCTGCCTGGCCCCGCTGTTGCAAGTCTGTTATTTATTACCTGGAGGCCTGTTC CATCCTGTCCTGGTCCTCCATAAAGCATTGTTTACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5056766 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5056766 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11377 same embryo
	EMAGE:11378 same embryo
	EMAGE:11375 same embryo
	EMAGE:11379 same embryo
	EurExpress:euxassay_006754 same experiment
	MGI:4827584 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source