EMAGE mouse gene expression spatial database

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EMAGE:11444

Itgb1bp1 integrin beta 1 binding protein 1 ( MGI:1306802)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006800. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006800_01	euxassay_006800_02	euxassay_006800_03	euxassay_006800_04

euxassay_006800_05	euxassay_006800_06	euxassay_006800_07	euxassay_006800_08	euxassay_006800_09

euxassay_006800_10	euxassay_006800_11	euxassay_006800_12	euxassay_006800_13	euxassay_006800_14

euxassay_006800_15	euxassay_006800_16	euxassay_006800_17	euxassay_006800_18	euxassay_006800_19

euxassay_006800_20	euxassay_006800_21	euxassay_006800_22	euxassay_006800_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11444_wholemount_strong.wlz

11444_wholemount_moderate.wlz

11444_wholemount_weak.wlz

11444_wholemount_possible.wlz

11444_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11444_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

left lung

strong

spotted

strong expression: see section 09 10 13 moderate expression: see section 06 07 08 11 12

right lung

strong

spotted

strong expression: see section 14 15 16 17 moderate expression: see section 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8216
Entity Detected:	Itgb1bp1, integrin beta 1 binding protein 1 ( MGI:1306802)
Sequence:	sense strand is shown >T8216 CTTCTCTGGATCGAGCTATGGTGAAAGATGTTTCGCAAAGGCAAGAAGAGACACAGCAGCAGCAGTTCCC AGAGCAGTGAAATCAGTACAAAGAGCAAGTCTGTGGATTCCAGCCTTGGAGGACTTTCAAGATCCAGCAC TGTCGCCAGCCTCGATACAGACTCCACTAAAAGCTCAGGACAGAGTAACAGCAATTTAGACACATGTGCT GAGTTCCGAATAAAGTATGTTGGTGCCATCGAGAAACTGGCCGTGTCTGAGGGGAAGAGCCTTGAAGGAC CACTAGACCTGATAAATTACATAGATGTCGCCCAGCAAGATGGAAAGTTGCCTTTTGTGCCTTTGGAAGA GGAGTTCATTCTGGGCGTTTCTAAGTACGGCATAAAAGTCTCGACCACGGATCAGCATGGTGTTCTGCAT AGGCATGCCCTGTATCTAATCATCCGGATGGTGTGTTACGATGACGGCCTGGGAGCTGGGAAAAGCTTGC TGGCACTCAAGACCACAGATGCAAGCAACGAAGAGTACAGCCTGTGGGTTTACCAGTGTAACAGCCTGGA GCAAGCCCAAGCAATCTGCAAGGTCTTATCCACAGCATTTGACTCTGTGTTGACCTCTGACAAGTCCTGA ACTCTGCAGGCAAGTGGAAACTGACCCACGGAGCTTACCTGTTCCTACATCTATCTGCAGCTGTGGACTA TTAGAGGGTGTAATGCCCTGACCCGGTTTTCTAAAGTGTAATGTATGAAATATTGGTTTTATACTAAAAG GTGTTAACAGTAGGTCAACTTCCAATAAATTACCTGCTTTCTGGAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1230640 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1230640 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11445 same embryo
	EMAGE:11443 same embryo
	EMAGE:11442 same embryo
	EMAGE:11441 same embryo
	EMAGE:11440 same embryo
	EurExpress:euxassay_006800 same experiment
	MGI:4825652 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source