EMAGE mouse gene expression spatial database

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EMAGE:11445

Pnpla3 patatin-like phospholipase domain containing 3 ( MGI:2151796)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006787. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006787_01	euxassay_006787_02	euxassay_006787_03	euxassay_006787_04

euxassay_006787_05	euxassay_006787_06	euxassay_006787_07	euxassay_006787_08	euxassay_006787_09

euxassay_006787_10	euxassay_006787_11	euxassay_006787_12	euxassay_006787_13	euxassay_006787_14

euxassay_006787_15	euxassay_006787_16	euxassay_006787_17	euxassay_006787_18	euxassay_006787_19

euxassay_006787_20	euxassay_006787_21	euxassay_006787_22	euxassay_006787_23	euxassay_006787_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11445_wholemount_strong.wlz

11445_wholemount_moderate.wlz

11445_wholemount_weak.wlz

11445_wholemount_possible.wlz

11445_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11445_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 15 16 17 weak expression: see section 18 19

left lung

moderate

regional

moderate expression: see section 07 08 09 10 11 12 weak expression: see section 04 05 06 13 14

right lung

moderate

regional

moderate expression: see section 16 17 20 weak expression: see section 15 18 19 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8233
Entity Detected:	Pnpla3, patatin-like phospholipase domain containing 3 ( MGI:2151796)
Sequence:	sense strand is shown >T8233 CCGGGAGCAGAGCTGAACTGCAGCGCCGCCCGGAGCTTCAAGCACCATGTATGACCCAGAGCGCCGCTGG AGCCTGTCGTTTGCAGGCTGCGGCTTCCTGGGCTTCTACCACGTCGGGGCTACGCTATGTCTGAGCGAGC GCGCCCCGCACCTCCTCCGCGATGCGCGCACTTTCTTTGGCTGCTCGGCCGGTGCACTGCACGCGGTCAC CTTCGTGTGCAGTCTCCCTCTCGGCCGTATAATGGAGATCCTCATGGACCTCGTGCGGAAAGCCAGGAGC CGCAACATCGGCACCCTCCACCCGTTCTTCAACATTAACAAGTGCATCAGAGACGGGCTCCAGGAGAGCC TCCCAGACAATGTCCACCAGGTCATTTCTGGCAAGGTTCACATCTCACTCACCAGGGTGTCGGATGGGGA GAACGTGCTGGTGTCTGAGTTCCATTCCAAAGACGAAGTCGTGGATGCCCTGGTGTGTTCCTGCTTCATT CCCCTCTTCTCTGGCCTAATCCCTCCTTCCTTCCGAGGCGAGCGGTACGTGGACGGAGGAGTGAGCGACA ACGTCCCTGTGCTGGATGCCAAAACCACCATCACGGTGTCACCTTTCTACGGTGAGCATGACATCTGCCC CAAAGTCAAGTCCACCAACTTCTTCCACGTGAATATCACCAACCTCAGCCTCCGCCTCTGCACTGGGAAC CTCCAACTTCTGACCAGAGCGCTCTTCCCGTCTGATGTGAAGGTGATGGGAGAGCTGTGCTATCAAGGGT ACCTGGACGCCTTCCGGTTCCTGGAGGAGAATGGCATCTGTAACGGGCCACAGCGCAGCCTGAGTCTGTC CTTGGTGGCGCCAGAAGCCTGCTTGGAAAATGGCAAACTTGTGGGAGACAAGGTGCCAGTCAGCCTATGC TTTACAGATGAGAACATCTGGGAGACACTGTCCCCCGAGCTCAGCACAGCTCTGAGTGAAGCGATTAAGG ACAGGGAGGGCTACCTGAGCAAAGTCTGCAACCTCCTGCCCGTCAGGATCCTGTCCTACATCATGCTGCC CTGCAGTCTGCCCGTGGAGTCGGCTATCGCTGCAGTCCACAGGCTGGTGACATGGCTCCCTGATATCCAG GATGATATCCAGTGGCTACAATGGGCGACATCCCAGGTTTGTGCCCGAATGACGATGTGCCTGCTCCCCT CTACCAGATCCCGAGCATCCAAGGATGACCATCGAATGCTCAAGCATGGCCATCACCCATCTCCCCACAA GCCTCAAGGCAACTCTGCTGGTTTGTAAATTGCTGGTCTCCGTGCTTCCAGTGAACTTGGACATTCTTCT CATGGTTGGTCCAGGAGAGGCCAAAGCTGAGGGCACCCTGCCTTCCACCCCCAGTCCAGCTTGACCTTTT ATCTGGAGCAACAGTGTCTAGATGATGGGTGGGTGAGGGGTGCTATACTGTCTGTCCCTCTGGGAAGGGT TCTGTTACTTTTGGAGGCAGCTAGGAAGTTTCTCTGTGCAGCTGCCCCCTGGTGCTGTGTGGTGACCTCA TTGCCTGTGACCCCAGGATCACAGGATCTGGGCTAAAGTGGTAGTCCATAGAAACCAAAGACAATGATTT GGTGTTTAGAAAGCTACTCTTGGTCTGGGTGAAGTCTGGTGCTTAAGGGCTATCACAAAGAGCGTGTCAA ACCATCTCTCAGCCTGTGAGTCAGTGGGGAGCCCAAGGGCATCAGTGTTTGGAAACTGGAATCCAAACCG GGCAATCTCGGAAGGAAACTGTTTAGGAATTGTGATGGGACGGGCCGTGGCTGTCTCTGAAAAGGGCCTG CCAGATAACTTATTACTTTTAAGGACACCTTTGGCTCTTACTAATTTATAAAGCATTTTATATAAACAAA AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1265861 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1265861 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11443 same embryo
	EMAGE:11442 same embryo
	EMAGE:11444 same embryo
	EMAGE:11441 same embryo
	EMAGE:11440 same embryo
	EurExpress:euxassay_006787 same experiment
	MGI:4827304 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source