EMAGE mouse gene expression spatial database

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EMAGE:11493

Chi3l3 chitinase 3-like 3 ( MGI:1330860)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006971. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006971_01	euxassay_006971_02	euxassay_006971_03	euxassay_006971_04

euxassay_006971_05	euxassay_006971_06	euxassay_006971_07	euxassay_006971_08	euxassay_006971_09

euxassay_006971_10	euxassay_006971_11	euxassay_006971_12	euxassay_006971_13	euxassay_006971_14

euxassay_006971_15	euxassay_006971_16	euxassay_006971_17	euxassay_006971_18	euxassay_006971_19

euxassay_006971_20	euxassay_006971_21	euxassay_006971_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11493_wholemount_strong.wlz

11493_wholemount_moderate.wlz

11493_wholemount_weak.wlz

11493_wholemount_possible.wlz

11493_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11493_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9943
Entity Detected:	Chi3l3, chitinase 3-like 3 ( MGI:1330860)
Sequence:	sense strand is shown >T9943 AGCAATCCTGAAGACACCATGGCCAAGCTCATTCTTGTCACAGGTCTGGCAATTCTTCTGAACGTACAGC TGGGATCTTCCTACCAGCTGATGTGCTACTATACCAGTTGGGCTAAGGACAGGCCAATAGAAGGGAGTTT CAAACCTGGTAATATTGACCCCTGCCTGTGTACTCACCTGATCTATGCCTTTGCTGGAATGCAGAATAAT GAGATCACTTACACACATGAGCAAGACTTGCGTGACTATGAAGCATTGAATGGTCTGAAAGACAAGAACA CTGAGCTAAAAACTCTCCTGGCCATTGGAGGATGGAAGTTTGGACCTGCCCCGTTCAGTGCCATGGTCTC TACTCCTCAGAACCGTCAGATATTCATTCAGTCAGTTATCAGATTCCTTCGTCAATATAACTTTGATGGC CTCAACCTGGACTGGCAGTACCCTGGGTCTCGAGGAAGCCCTCCTAAGGACAAACATCTCTTCAGTGTTC TGGTGAAGGAAATGCGTAAAGCTTTTGAGGAAGAATCTGTGGAGAAAGACATTCCAAGGCTGCTACTCAC TTCCACAGGAGCAGGAATCATTGACGTAATCAAGTCTGGGTACAAGATCCCTGAACTGTCTCAGTCTCTT GACTATATTCAGGTCATGACATATGATCTCCATGATCCTAAGGATGGCTACACTGGAGAAAATAGTCCCC TCTATAAATCTCCATATGACATTGGAAAGAGTGCTGATCTCAATGTGGATTCAATCATTTCCTACTGGAA GGACCATGGAGCAGCTTCTGAGAAGCTCATTGTGGGATTTCCAGCATATGGGCATACCTTTATCCTGAGT GACCCTTCTAAGACTGGAATTGGTGCCCCTACAATTAGTACTGGCCCACCAGGAAAGTACACAGATGAAT CAGGACTCCTGGCTTACTATGAGGTTTGTACATTTCTGAATGAAGGAGCCACTGAGGTCTGGGATGCCCC CCAGGAAGTACCCTATGCCTATCAGGGTAATGAGTGGGTTGGTTATGACAATGTCAGGAGCTTCAAGTTG AAGGCTCAGTGGCTCAAGGACAACAATTTAGGAGGTGCCGTGGTCTGGCCCCTGGACATGGATGACTTCA GTGGTTCTTTCTGTCACCAGAGACATTTCCCTCTGACATCTACTTTAAAGGGAGATCTCAATATACACAG TGCAAGTTGCAAGGGCCCTTATTGAGAGGAGCTTTACACAATGATTTGTCCTTGAAACTCTCAGAATAAG ATCAAGTTCAACGGTTTTTCCACAGTGCATTCTGCATCATGCTTCCATGGAGAATAATAGAAATAAGTCA TGAACTTTCCTAAATTGAATCCCAGAGTAGTACTAAGATGGATGTCTTGTCTGCTGTACCAGCTGGGAAG AAACAAAAAATGCTCTTCATCTGTCAGCTTTGGCTAAGCTCTGAACATCTTTTGCTTCCTGTAAAACCAC CATGCTTGTTTCTTGCTCTCACAATAAATTCCACATTCATAGCAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:30298306 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30298306 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11492 same embryo
	EMAGE:11494 same embryo
	EMAGE:11490 same embryo
	EMAGE:11491 same embryo
	EMAGE:11495 same embryo
	EurExpress:euxassay_006971 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source