EMAGE mouse gene expression spatial database

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EMAGE:11513

Mkl2 MKL/myocardin-like 2 ( MGI:3050795)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006821. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006821_01	euxassay_006821_02	euxassay_006821_03	euxassay_006821_04

euxassay_006821_05	euxassay_006821_06	euxassay_006821_07	euxassay_006821_08	euxassay_006821_09

euxassay_006821_10	euxassay_006821_11	euxassay_006821_12	euxassay_006821_13	euxassay_006821_14

euxassay_006821_15	euxassay_006821_16	euxassay_006821_17	euxassay_006821_18	euxassay_006821_19

euxassay_006821_20	euxassay_006821_21	euxassay_006821_22	euxassay_006821_23	euxassay_006821_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11513_wholemount_strong.wlz

11513_wholemount_moderate.wlz

11513_wholemount_weak.wlz

11513_wholemount_possible.wlz

11513_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11513_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8227
Entity Detected:	Mkl2, MKL/myocardin-like 2 ( MGI:3050795)
Sequence:	sense strand is shown >T8227 CGGCGAGCGGGTTCATAATTAAGGTGCTAAGGAAGAGAATGTAAGACAGGAAAATAAATATTTTTACTGA GGCTGCGTAAAAGAGGTGTCTCAGTGGCTGGAACACAATGGATCACACAGGAGCCATAGACACAGAGGAA GAGCTGGGGCCTCTAGCCCATCTTGCACCAAGTCCTCAAAGTGAGTCTGTGGCTCACGAATTCCAGGAAC TGTCTCTACAGTCCAGCCAGCACTTACCCACTCTGAACGAACGGAAAAATGGAAGTATAGTCTTTGGGGA GTCAGGACTGCAGCTTCCTCTGCCGGCTCAGTCTGTGAGCACAGGACATAATAAGAAAAGCAAGAAGCCT CATTTGCCACTAGTCTTGGGTTCCTGCAGGCTGCATTTAGAACCTGCCAGGTGTTTGGATGGTTGACAAG CTGCAGACTGGGAAGTTGATAGCAACAAAATTAGAACACTACCTGGAATAGACGCCTCCTCCTGACAGGG CAACTGTGCTGCTTGCTGACTGTGCAGCTTGCCCAGCCTCTCATGGAATACTGCACTTAAAAACAGTCAG TCCACATCTCTCATGGTGTTGAAAATTGGCACTTCGAGGATAGAAATAAAGATACTTTTCTTTTTACAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1247408 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1247408 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11514 same embryo
	EMAGE:11516 same embryo
	EMAGE:11517 same embryo
	EMAGE:11515 same embryo
	EMAGE:11512 same embryo
	EurExpress:euxassay_006821 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source