EMAGE mouse gene expression spatial database

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EMAGE:11514

Dpp9 dipeptidylpeptidase 9 ( MGI:2443967)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006816. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006816_01	euxassay_006816_02	euxassay_006816_03	euxassay_006816_04

euxassay_006816_05	euxassay_006816_06	euxassay_006816_07	euxassay_006816_08	euxassay_006816_09

euxassay_006816_10	euxassay_006816_11	euxassay_006816_12	euxassay_006816_13	euxassay_006816_14

euxassay_006816_15	euxassay_006816_16	euxassay_006816_17	euxassay_006816_18	euxassay_006816_19

euxassay_006816_20	euxassay_006816_21	euxassay_006816_22	euxassay_006816_23	euxassay_006816_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11514_wholemount_strong.wlz

11514_wholemount_moderate.wlz

11514_wholemount_weak.wlz

11514_wholemount_possible.wlz

11514_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11514_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7352
Entity Detected:	Dpp9, dipeptidylpeptidase 9 ( MGI:2443967)
Sequence:	sense strand is shown >T7352 GCTGGCGGGTCCCCCCGGTGTCCGCCGCCTTCGCTGTCCCCCCGCGCCCGCCACTTCCGGGCCCTCGTCT TGGGCATGGAGCGTCGCGTGTGAGGCGCCGCCGGCCCCGGGGACGCCCTGCTCAGCTTGTCCTCCGCGCC ACGCTCGGGTCCGTGCCCCTCGCCTGCAGCTGCCATGGAAGCTCCTTGGACCCTTTAGAAGCTTCTCGCC GCCATCACAGGAGCCCCAGAGGATGTGCAGCGGGGTCTCCCCAGTTGAGCAGGTGGCCGCAGGGGACATG GATGACACGGCAGCACGCTTCTGTGTGCAGAAGCACTCGTGGGATGGGCTGCGTAGCATTATCCACGGCA GTCGCAAGTCCTCGGGCCTCATTGTCAGCAAGGCCCCCCACGACTTCCAGTTTGTGCAGAAGCCTGACGA GTCTGGCCCCCACTCTCACCGTCTCTATTACCTCGGAATGCCTTACGGCAGCCGTGAGAACTCCCTCCTC TACTCCGAGATCCCCAAGAAAGTGCGGAAGGAGGCCCTGCTGCTGCTGTCCTGGAAGCAGATGCTGGACC ACTTCCAGGCCACACCCCACCATGGTGTCTACTCCCGAGAGGAGGAGCTACTGCGGGAGCGCAAGCGCCT GGGCGTCTTCGGAATCACCTCTTATGACTTCCACAGTGAGAGCGGCCTCTTCCTCTTCCAGGCCAGCAAT AGCCTGTTCCACTGCAGGGATGGTGGCAAGAATGGCTTTATGGTGTCCCCGATGAAGCCACTGGAGATCA AGACTCAGTGTTCTGGGCCACGCATGGACCCCAAAATCTGCCCCGCAGACCCTGCCTTCTTTTCCTTCAT CAACAACAGTGATCTGTGGGTGGCAAACATCGAGACTGGGGAGGAACGGCGGCTCACCTTCTGTCACCAG GGTTCAGCTGGTGTCCTGGACAATCCCAAATCAGCAGGCGTGGCCACCTTTGTCATCCAGGAGGAGTTCG ACCGCTTCACTGGGTGCTGGTGGTGCCCCACGGCCTCTTGGGAAGGCTCCGAAGGTCTCAAGACGCTGCG CATCCTATATGAGGAAGTGGACGAGTCTGAAGTGGAGGTCATTCATGTGCCCTCCCCCGCCCTGGAGGAG AGGAAGACGGACTCCTACCGCTACCCCAGGACAGGCAGCAAGAACCCCAAGATTGCCCTGAAGCTGGCTG AGCTCCAGACGGACCATCAGGGCAAAATCGTGTCAAGCTGCGAGAAGGAACTGGTACAGCCATTCAGCTC CCTTTTCCCCAAAGTGGAGTACATCGCCCGGGCTGGCTGGACACGGGACGGCAAATATGCCTGGGCCATG TTCCTGGACCGTCCCCAGCAACGGCTTCAGCTTGTCCTCCTGCCCCCTGCTCTCTTCATCCCGGCCGTTG AGAGTGAGGCCCAGCGGCAGGCAGCTGCCAGAGCCGTCCCCAAGAATGTGCAGCCCTTTGTCATCTATGA AGAAGTCACCAATGTCTGGATCAACGTCCACGACATCTTCCACCCGTTTCCTCAGGCTGAGGGCCAGCAG GACTTTTGTTTCCTTCGTGCCAACGAATGCAAGACTGGCTTCTGCCACCTGTACAGGGTCACAGTGGAAC TTAAAACCAAGGACTATGACTGGACGGAACCCCTCAGCCCTACAGAAGATGAGTTTAAGTGCCCCATCAA GGAGGAGGTCGCCCTGACCAGTGGCGAGTGGGAGGTCTTGTCGAGGCATGGCTCCAAGATCTGGGTCAAC GAGCAGACGAAGCTGGTGTACTTTCAAGGTACAAAGGACACACCGCTGGAACATCACCTCTATGTGGTCA GCTACGAGTCAGCAGGCGAGATCGTGCGGCTCACCACGCTCGGCTTCTCCCACAGCTGCTCCATGAGCCA GAGCTTCGACATGTTCGTGAGTCACTACAGCAGTGTGAGCACGCCACCCTGTGTACATGTGTACAAGCTG AGCGGCCCCGATGATGACCCACTGCACAAGCAACCACGCTTCTGGGCCAGCATGATGGAGGCAGCCAATT GCCCCCCAGACTATGTGCCCCCTGAGATCTTCCACTTCCACACCCGTGCAGACGTGCAGCTCTACGGCAT GATCTACAAGCCACACACCCTGCAACCTGGGAGGAAGCACCCCACTGTGCTCTTTGTCTATGGGGGCCCA CAGGTGCAGTTGGTGAACAACTCCTTTAAGGGCATCAAATACCTGCGGCTAAATACACTGGCATCCTTGG GCTATGCTGTGGTGGTGATCGATGGTCGGGGCTCCTGTCAGCGGGGCCTGCACTTCGAGGGGGCCCTGAA AAATCAAATGGGCCAGGTGGAGATTGAGGACCAGGTGGAAGGCTTGCAGTACGTGGCTGAGAAGTATGGC TTCATTGACTTGAGCCGAGTCGCCATCCATGGCTGGTCCTACGGCGGCTTCCTCTCACTCATGGGGCTCA TCCACAAGCCACAAGTGTTCAAGGTAGCCATTGCGGGCGCTCCTGTCACTGTGTGGATGGCCTATGACAC AGGGTACACGGAACGATACATGGATGTCCCCGAAAATAACCAGCAAGGCTATGAGGCAGGGTCTGTAGCC CTGCATGTGGAGAAGCTGCCCAATGAGCCTAACCGCCTGCTTATCCTCCACGGCTTCCTGGACGAGAACG TTCACTTCTTCCACACAAATTTCCTGGTGTCCCAGCTGATCCGAGCAGGAAAGCCATACCAGCTTCAGAT CTACCCAAACGAGAGACATAGCATCCGCTGCCGCGAGTCCGGAGAGCATTACGAGGTGACGCTGCTGCAC TTTCTGCAGGAACACCTGTGACCTCAGTCCCGACTCCTGACGCCACCGCTGCTCTTCTTGCGTTTTTGTA ATCTTTTCATTTTTGAAGCTTCCAATTTGCTTGCTGCTGCTGCTGCCTGGGGGCCAGGACAGAGGTAGTG GCGGCCCCCATGCCGCCCTCCTTGAGCTGGTGAGGAGAAGTCGCCATTGAGCACACAACCTCCACCAGAC TGCCATGGCCCCGAACCTGCAATTCCATCCTAGCGCAGAAGCATGTGCCTGCCACCTGCTGCCCCTGCAG AGTCATGTGTGTTTGTGGTGGGCATTTTAAATAATTATTTAAAAGACAGGAAGTAAGCGGTACCGAGCAA TGAAACTGAAGGTACAGCACTGGGCGTCTGGGGACCCCACGCTCTCCCAACGCCCAGACTATGTGGAGCT GCCAAGCCCCTGTCTGGGCACCTCTGCCCTGCCTGTCTGCTGCCCGGATCCTCCTCACTTAGCACCTAGG GGTGTCAGGGTCGGGAGTAGGACCTGTCCTGACCTCAGGGTTATATATAGCCCTTCCCCACTCCCTCCTA CGAGAGTTCTGGCATAAAGAAGTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4191886 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4191886 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11513 same embryo
	EMAGE:11516 same embryo
	EMAGE:11517 same embryo
	EMAGE:11515 same embryo
	EMAGE:11512 same embryo
	EurExpress:euxassay_006816 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source